LOCUS       pOME0180      2171 bp    DNA     linear   UNA 30-Jan-2014
DEFINITION  KSHV ORF50 hyp TAGGED; vector pDEST47
SOURCE      UNC166-10 (KSHV ORF50 hyp TAGGED)
COMMENT     Plasmid synthesized by BioBasic
            Sequence verified via Sanger sequencing through Genewiz
            pOME= plasmid of the Orfeome project
            hyp= hypthetical gene
              
FEATURES             Location/Qualifiers
     Site            join(1..6,1^2)
                     /site_type="restriction site"
                     /note="Name: SalI"
                     /note="Pattern: gtcgac"
                     /note="cut_0_on_positive_strand: 1^2"
                     /note="cut_0_on_negative_strand: 5^6"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=SalI
     Site            1..6
                     /site_type="mutagenized site"
                     /label="5' RE tag"
     Site            7..12
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             13..2154
                     /cds_type=ORF
                     /note="Length: 2142"
                     /note="Found at strand: positive"
                     /note="Start codon: atg"
     Site            2086..2151
                     /site_type="mutagenized site"
                     /label="3X FLAG tag"
     Site            2152..2157
                     /site_type="mutagenized site"
                     /label="double stop"
     Site            join(2158..2163,2158^2159)
                     /site_type="restriction site"
                     /note="Name: XhoI"
                     /note="Pattern: ctcgag"
                     /note="cut_0_on_positive_strand: 2158^2159"
                     /note="cut_0_on_negative_strand: 2162^2163"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=XhoI
     Site            2158..2171
                     /site_type="mutagenized site"
                     /label="3' RE tag"
     Site            join(2164..2171,2165^2166)
                     /site_type="restriction site"
                     /note="Name: NotI"
                     /note="Pattern: gcggccgc"
                     /note="cut_0_on_positive_strand: 2165^2166"
                     /note="cut_0_on_negative_strand: 2169^2170"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=NotI
ORIGIN
        1 gtcgacgcca ccatggcgca agatgacaag ggtaagaagc ttcggcggtc ctgtgtggaa
       61 agcttcgtcg gcctctcgga cgaactgaag gcccaactct accagtgtgt gctccttata
      121 aatgacgcat acgaaacaat ctacgatccc agtgacctaa atagagtggt ggaagatgtg
      181 tgcattcgga ttatgaaaga atgttccaag cttggtgcgc tatgtggtct gtttacagac
      241 attaacatgt ttaacctttt ctgctttttt cgtgcctctc gaatgaggac caaaggcgcg
      301 gccgggtaca acgtgccatg cgcagaggca tcccaaggca ttattcggat cctcacggag
      361 aggatcttat tctgcacaga aaaggcattt ctgacagccg catgcagcgg ggtgagcctg
      421 cctccagcca tatgtaagct actacacgaa atatacactg aaatgaaggc caaatgcctg
      481 ggggcctgga ggcgactcgt ctgcaatcgg aggcccatta tgatattaac ctcttcccta
      541 ctgaagctct acaacacgta cgataccgcc gggctgctct ctgagcagtc cagggccctc
      601 tgccttttgg ttttccaacc ggtctacctt ccgaggatta tggcgccgct ggagatcatg
      661 accaagggtc agctcgcccc tgaaaacttt tacagcatca ccggttctgc tgagaaacgc
      721 cggccaatta ccaccggcaa ggtcactgga ctgtcctatc caggaagcgg tctcatgcca
      781 gaatctttaa ttttgccaat cctggagcca ggactgttgc cggcttccat ggtagacctc
      841 agcgatgtgc tggcaaaacc cgccgttatt ctgagcgccc ctgccctgag ccagtttgtc
      901 attagcaaac cccatcccaa catgccgcac accgtcagca tcatcccctt taacccatcg
      961 ggtacagacc cggcgtttat tagtacgtgg caggccgcgt cacagaatat ggtgtacaac
     1021 acatccaccg cgcccttaaa accggccacc ggtagttcac agacggtgtc agtcaaggcg
     1081 gttgctcaag gggccgtgat tactgcgaca acggtgccgc aggcaatgcc agcgcggggt
     1141 accggagggg agttgcctgt aatgtcagcg tccactcctg caagagatca ggtcgctgca
     1201 tgttttgtcg cagagaacac cggagattct cccgacaacc cgagctcttt cctgacgtca
     1261 tgtcaccctt gcgatccgaa cacggttata gtggcccagc aatttcaacc accgcaatgc
     1321 gttacgttgt tgcaggttac ctgtgccccc tcttcgacac caccccccga ttcaacagtc
     1381 cgggccccgg tggtgcagtt gccaacagta gtccctctgc cggccagcgc gttcctcccg
     1441 gcgctcgccc aaccagaagc ctcgggcgaa gagcttccgg gcggtcatga cggagaccaa
     1501 ggtgtgccgt gtagagattc aacggcggcg gctacggcgg cagaggcgac aacacccaaa
     1561 cgaaagcaga gaagcaaaga gaggagctca aagaagcgta aggctttgac cgtgccagaa
     1621 gccgacacca cgccatcgac cacgacacct ggtacctctt tgggatcaat taccaccccc
     1681 caggatgtgc acgccacgga tgtcgccacg tctgagggac catcggaggc acaacccccg
     1741 ctactgtcgt tacccccgcc actggacgta gatcagagtc tattcgccct gttagacgaa
     1801 gcgggccctg aaacatggga tgtcgggtcg cctctctccc ccactgacga cgcgctgttg
     1861 tccagtattc tgcaaggact gtaccagctg gacacgccac cgcctctgcg gtcaccctcc
     1921 cccgcttcct tcggcccgga gtctccggcg gatataccgt caccttctgg tggagagtat
     1981 acgcaactgc aaccggtcag ggcgacctcg gcgacgcccg ctaacgaggt acaggagtcc
     2041 ggcacactgt accagctgca ccaatggcgt aattacttcc gagacgacta caaagaccat
     2101 gacggtgatt ataaagatca tgatatcgat tacaaggatg acgatgacaa gtagtaactc
     2161 gaggcggccg c
//
