LOCUS pOME0180 2171 bp DNA linear UNA 30-Jan-2014 DEFINITION KSHV ORF50 hyp TAGGED; vector pDEST47 SOURCE UNC166-10 (KSHV ORF50 hyp TAGGED) COMMENT Plasmid synthesized by BioBasic Sequence verified via Sanger sequencing through Genewiz pOME= plasmid of the Orfeome project hyp= hypthetical gene FEATURES Location/Qualifiers Site join(1..6,1^2) /site_type="restriction site" /note="Name: SalI" /note="Pattern: gtcgac" /note="cut_0_on_positive_strand: 1^2" /note="cut_0_on_negative_strand: 5^6" /note="inhibited_by: 5': N6-methyladenosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label=SalI Site 1..6 /site_type="mutagenized site" /label="5' RE tag" Site 7..12 /site_type="mutagenized site" /label="Kozak sequence" CDS 13..2154 /cds_type=ORF /note="Length: 2142" /note="Found at strand: positive" /note="Start codon: atg" Site 2086..2151 /site_type="mutagenized site" /label="3X FLAG tag" Site 2152..2157 /site_type="mutagenized site" /label="double stop" Site join(2158..2163,2158^2159) /site_type="restriction site" /note="Name: XhoI" /note="Pattern: ctcgag" /note="cut_0_on_positive_strand: 2158^2159" /note="cut_0_on_negative_strand: 2162^2163" /note="inhibited_by: 5': N6-methyladenosine" /note="site_type: other" /note="restriction site" /label=XhoI Site 2158..2171 /site_type="mutagenized site" /label="3' RE tag" Site join(2164..2171,2165^2166) /site_type="restriction site" /note="Name: NotI" /note="Pattern: gcggccgc" /note="cut_0_on_positive_strand: 2165^2166" /note="cut_0_on_negative_strand: 2169^2170" /note="inhibited_by: 5': N4-methylcytosine" /note="site_type: other" /note="restriction site" /label=NotI ORIGIN 1 gtcgacgcca ccatggcgca agatgacaag ggtaagaagc ttcggcggtc ctgtgtggaa 61 agcttcgtcg gcctctcgga cgaactgaag gcccaactct accagtgtgt gctccttata 121 aatgacgcat acgaaacaat ctacgatccc agtgacctaa atagagtggt ggaagatgtg 181 tgcattcgga ttatgaaaga atgttccaag cttggtgcgc tatgtggtct gtttacagac 241 attaacatgt ttaacctttt ctgctttttt cgtgcctctc gaatgaggac caaaggcgcg 301 gccgggtaca acgtgccatg cgcagaggca tcccaaggca ttattcggat cctcacggag 361 aggatcttat tctgcacaga aaaggcattt ctgacagccg catgcagcgg ggtgagcctg 421 cctccagcca tatgtaagct actacacgaa atatacactg aaatgaaggc caaatgcctg 481 ggggcctgga ggcgactcgt ctgcaatcgg aggcccatta tgatattaac ctcttcccta 541 ctgaagctct acaacacgta cgataccgcc gggctgctct ctgagcagtc cagggccctc 601 tgccttttgg ttttccaacc ggtctacctt ccgaggatta tggcgccgct ggagatcatg 661 accaagggtc agctcgcccc tgaaaacttt tacagcatca ccggttctgc tgagaaacgc 721 cggccaatta ccaccggcaa ggtcactgga ctgtcctatc caggaagcgg tctcatgcca 781 gaatctttaa ttttgccaat cctggagcca ggactgttgc cggcttccat ggtagacctc 841 agcgatgtgc tggcaaaacc cgccgttatt ctgagcgccc ctgccctgag ccagtttgtc 901 attagcaaac cccatcccaa catgccgcac accgtcagca tcatcccctt taacccatcg 961 ggtacagacc cggcgtttat tagtacgtgg caggccgcgt cacagaatat ggtgtacaac 1021 acatccaccg cgcccttaaa accggccacc ggtagttcac agacggtgtc agtcaaggcg 1081 gttgctcaag gggccgtgat tactgcgaca acggtgccgc aggcaatgcc agcgcggggt 1141 accggagggg agttgcctgt aatgtcagcg tccactcctg caagagatca ggtcgctgca 1201 tgttttgtcg cagagaacac cggagattct cccgacaacc cgagctcttt cctgacgtca 1261 tgtcaccctt gcgatccgaa cacggttata gtggcccagc aatttcaacc accgcaatgc 1321 gttacgttgt tgcaggttac ctgtgccccc tcttcgacac caccccccga ttcaacagtc 1381 cgggccccgg tggtgcagtt gccaacagta gtccctctgc cggccagcgc gttcctcccg 1441 gcgctcgccc aaccagaagc ctcgggcgaa gagcttccgg gcggtcatga cggagaccaa 1501 ggtgtgccgt gtagagattc aacggcggcg gctacggcgg cagaggcgac aacacccaaa 1561 cgaaagcaga gaagcaaaga gaggagctca aagaagcgta aggctttgac cgtgccagaa 1621 gccgacacca cgccatcgac cacgacacct ggtacctctt tgggatcaat taccaccccc 1681 caggatgtgc acgccacgga tgtcgccacg tctgagggac catcggaggc acaacccccg 1741 ctactgtcgt tacccccgcc actggacgta gatcagagtc tattcgccct gttagacgaa 1801 gcgggccctg aaacatggga tgtcgggtcg cctctctccc ccactgacga cgcgctgttg 1861 tccagtattc tgcaaggact gtaccagctg gacacgccac cgcctctgcg gtcaccctcc 1921 cccgcttcct tcggcccgga gtctccggcg gatataccgt caccttctgg tggagagtat 1981 acgcaactgc aaccggtcag ggcgacctcg gcgacgcccg ctaacgaggt acaggagtcc 2041 ggcacactgt accagctgca ccaatggcgt aattacttcc gagacgacta caaagaccat 2101 gacggtgatt ataaagatca tgatatcgat tacaaggatg acgatgacaa gtagtaactc 2161 gaggcggccg c //