LOCUS pOME0182R 788 bp DNA linear UNA 30-Jan-2014 DEFINITION KSHV K8.1 glycoprotein hyp TAGGED; vector pDEST47 SOURCE UNC166-12 (KSHV K8.1 glycoprotein hyp TAGGED) COMMENT Plasmid synthesized by BioBasic Sequence verified via Sanger sequencing through Genewiz pOME= plasmid of the Orfeome project hyp= hypothetical FEATURES Location/Qualifiers Site join(1..6,1^2) /site_type="restriction site" /note="Name: SalI" /note="Pattern: gtcgac" /note="cut_0_on_positive_strand: 1^2" /note="cut_0_on_negative_strand: 5^6" /note="inhibited_by: 5': N6-methyladenosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label=SalI Site 1..12 /site_type="mutagenized site" /label="5' RE tag" Site join(7..12,11^12) /site_type="restriction site" /note="Name: KpnI" /note="Pattern: ggtacc" /note="cut_0_on_positive_strand: 11^12" /note="cut_0_on_negative_strand: 7^8" /note="inhibited_by: 5': N6-methyladenosine" /note="site_type: other" /note="restriction site" /label=KpnI Site 13..18 /site_type="mutagenized site" /label="Kozak sequence" Site 703..768 /site_type="mutagenized site" /label="3x FLAG tag" Site 769..774 /site_type="mutagenized site" /label="double stop" Site join(775..780,775^776) /site_type="restriction site" /note="Name: XhoI" /note="Pattern: ctcgag" /note="cut_0_on_positive_strand: 775^776" /note="cut_0_on_negative_strand: 779^780" /note="inhibited_by: 5': N6-methyladenosine" /note="site_type: other" /note="restriction site" /label=XhoI Site 775..788 /site_type="mutagenized site" /label="3' RE tag" Site join(781..788,782^783) /site_type="restriction site" /note="Name: NotI" /note="Pattern: gcggccgc" /note="cut_0_on_positive_strand: 782^783" /note="cut_0_on_negative_strand: 786^787" /note="inhibited_by: 5': N4-methylcytosine" /note="site_type: other" /note="restriction site" /label=NotI ORIGIN 1 gtcgacggta ccgccaccat gagttccaca cagattcgca cagaaatccc tgtggcgctc 61 ctaatcctat gcctttgtct ggtggcgtgc catgccaatt gtcccacgta tcgttcgcat 121 ttgggattct ggcaagaggg ttggagtgga caggtttatc aggactggct aggcaggatg 181 aactgttcct acgagaatat gacggcccta gaggccgtct ccctaaacgg gaccagacta 241 gcagctggat ctccgtcgag tgagtatcca aatgtctccg tatctgttga agatacgtct 301 gcctctgggt ctggagaaga tgcaatagat gaatcggggt cgggggagga agagcgtccc 361 gtgacctccc acgtgacttt tatgacacaa agcgtccagg ccaccacaga actgaccgat 421 gccttaatat cagccttttc aggatcatat tcatctgggg aaccatccag gaccacgcga 481 attcgcgtat caccggtcgc agaaaacggc agaaatagtg gtgctagtaa ccgtgtgcca 541 ttttctgcca ccactacaac gactagagga agagacgcgc actacaatgc agaaatacgg 601 acccatcttt acatactatg ggctgtgggt ttattgctgg gacttgtcct tatactttac 661 ctgtgcgttc cacgatgccg gcgtaagaaa ccctacatag tggactacaa agaccatgac 721 ggtgattata aagatcatga tatcgattac aaggatgacg atgacaagta gtaactcgag 781 gcggccgc //