LOCUS       pOME0182R       788 bp    DNA     linear   UNA 30-Jan-2014
DEFINITION  KSHV K8.1 glycoprotein hyp TAGGED; vector pDEST47
SOURCE      UNC166-12 (KSHV K8.1 glycoprotein hyp TAGGED)
COMMENT     Plasmid synthesized by BioBasic
            Sequence verified via Sanger sequencing through Genewiz
            pOME= plasmid of the Orfeome project
            hyp= hypothetical 
FEATURES             Location/Qualifiers
     Site            join(1..6,1^2)
                     /site_type="restriction site"
                     /note="Name: SalI"
                     /note="Pattern: gtcgac"
                     /note="cut_0_on_positive_strand: 1^2"
                     /note="cut_0_on_negative_strand: 5^6"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=SalI
     Site            1..12
                     /site_type="mutagenized site"
                     /label="5' RE tag"
     Site            join(7..12,11^12)
                     /site_type="restriction site"
                     /note="Name: KpnI"
                     /note="Pattern: ggtacc"
                     /note="cut_0_on_positive_strand: 11^12"
                     /note="cut_0_on_negative_strand: 7^8"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=KpnI
     Site            13..18
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     Site            703..768
                     /site_type="mutagenized site"
                     /label="3x FLAG tag"
     Site            769..774
                     /site_type="mutagenized site"
                     /label="double stop"
     Site            join(775..780,775^776)
                     /site_type="restriction site"
                     /note="Name: XhoI"
                     /note="Pattern: ctcgag"
                     /note="cut_0_on_positive_strand: 775^776"
                     /note="cut_0_on_negative_strand: 779^780"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=XhoI
     Site            775..788
                     /site_type="mutagenized site"
                     /label="3' RE tag"
     Site            join(781..788,782^783)
                     /site_type="restriction site"
                     /note="Name: NotI"
                     /note="Pattern: gcggccgc"
                     /note="cut_0_on_positive_strand: 782^783"
                     /note="cut_0_on_negative_strand: 786^787"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=NotI
ORIGIN
        1 gtcgacggta ccgccaccat gagttccaca cagattcgca cagaaatccc tgtggcgctc
       61 ctaatcctat gcctttgtct ggtggcgtgc catgccaatt gtcccacgta tcgttcgcat
      121 ttgggattct ggcaagaggg ttggagtgga caggtttatc aggactggct aggcaggatg
      181 aactgttcct acgagaatat gacggcccta gaggccgtct ccctaaacgg gaccagacta
      241 gcagctggat ctccgtcgag tgagtatcca aatgtctccg tatctgttga agatacgtct
      301 gcctctgggt ctggagaaga tgcaatagat gaatcggggt cgggggagga agagcgtccc
      361 gtgacctccc acgtgacttt tatgacacaa agcgtccagg ccaccacaga actgaccgat
      421 gccttaatat cagccttttc aggatcatat tcatctgggg aaccatccag gaccacgcga
      481 attcgcgtat caccggtcgc agaaaacggc agaaatagtg gtgctagtaa ccgtgtgcca
      541 ttttctgcca ccactacaac gactagagga agagacgcgc actacaatgc agaaatacgg
      601 acccatcttt acatactatg ggctgtgggt ttattgctgg gacttgtcct tatactttac
      661 ctgtgcgttc cacgatgccg gcgtaagaaa ccctacatag tggactacaa agaccatgac
      721 ggtgattata aagatcatga tatcgattac aaggatgacg atgacaagta gtaactcgag
      781 gcggccgc
//