LOCUS       pOME0184      1463 bp    DNA     linear   UNA 30-Jan-2014
DEFINITION  KSHV ORF57 hyp TAGGED;  vector pDEST47
SOURCE      UNC166-16 (KSHV ORF57 TAGGED)
COMMENT     5' end cut only with SalI to avoid internal KpnI site  
            Plasmid synthesized by BioBasic
            Sequence verified via Sanger sequencing through Genewiz
            pOME= plasmid of the Orfeome project
            hyp= hypothetical 
FEATURES             Location/Qualifiers
     Site            join(1..6,1^2)
                     /site_type="restriction site"
                     /note="Name: SalI"
                     /note="Pattern: gtcgac"
                     /note="cut_0_on_positive_strand: 1^2"
                     /note="cut_0_on_negative_strand: 5^6"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=SalI
     Site            1..6
                     /site_type="mutagenized site"
                     /label="5' RE tag"
     Site            7..12
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             13..1446
                     /cds_type=ORF
                     /note="Length: 1434"
                     /note="Found at strand: positive"
                     /note="Start codon: atg"
     Site            1378..1443
                     /site_type="mutagenized site"
                     /label="3X FLAG tag"
     Site            1444..1449
                     /site_type="mutagenized site"
                     /label="double stop"
     Site            join(1450..1455,1450^1451)
                     /site_type="restriction site"
                     /note="Name: XhoI"
                     /note="Pattern: ctcgag"
                     /note="cut_0_on_positive_strand: 1450^1451"
                     /note="cut_0_on_negative_strand: 1454^1455"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=XhoI
     Site            1450..1463
                     /site_type="mutagenized site"
                     /label="3' RE tag"
     Site            join(1456..1463,1457^1458)
                     /site_type="restriction site"
                     /note="Name: NotI"
                     /note="Pattern: gcggccgc"
                     /note="cut_0_on_positive_strand: 1457^1458"
                     /note="cut_0_on_negative_strand: 1461^1462"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=NotI
ORIGIN
        1 gtcgacgcca ccatggtaca agcaatgata gacatggaca ttatgaaggg catcctagag
       61 gactctgtgt cctcctctga gtttgacgaa tcgagggacg acgagacgga cgcaccgaca
      121 ctggaagacg agcaattgtc cgaacccgcc gagcctccgg cagacgagcg catccgtggt
      181 acccagtcgg cccagggaat cccacccccc ctgggccgca tcccaaaaaa atctcaaggt
      241 cgttctcaac tgcgcagtga gatccagttt tgctccccac tgtctcgacc caggtccccc
      301 tcaccagtaa acaggtacgg taaaaaaatc aagtttggaa ccgccggtca aaacacacgt
      361 cctccccctg aaaagcgtcc tcggcgcaga ccacgcgacc gcctacaata cggcagaaca
      421 acacggggcg gacagtgtcg cgctgcaccg aagcgagcga cccgccgtcc gcaggtcaat
      481 tgccagcggc aggatgacga cgtcagacag ggtgtgtctg acgccgtaaa gaaactcaga
      541 ctccctgcga gcatgataat tgacggtgag agcccccgct tcgacgactc gatcatcccc
      601 cgccaccatg gcgcatgttt caatgtcttc attcccgccc caccatccca cgtcccggag
      661 gtgtttacgg acagggatat caccgctctc ataagagcag ggggcaaaga cgacgaactc
      721 ataaacaaaa aaatcagcgc aaaaaagatt gaccacctcc acagacagat gctgtctttt
      781 gtgaccagcc gccataatca agcgtactgg gtgagttgcc gtcgagaaac cgcagccgcc
      841 ggaggcctgc aaacgcttgg ggctttcgtg gaggaacaaa tgacgtgggc ccagacggtt
      901 gtgcgccacg gggggtggtt tgatgagaag gacatagata taattttgga caccgcaata
      961 tttgtctgca atgcgtttgt taccagattt agattacttc atctttcctg cgtttttgac
     1021 aagcagagcg agctagcact gatcaaacag gtggcatatt tggtagcgat gggaaaccgc
     1081 ttagtagagg catgtaacct tcttggcgag gtcaagctta acttcagggg agggctgctc
     1141 ttggcctttg tcctaactat cccaggcatg cagagtcgca gaagtatttc tgcgcgcgga
     1201 caggagctgt ttagaacact tctggaatac tacaggccag gggatgtgat ggggctacta
     1261 aacgtgatag taatggaaca tcacagcttg tgcagaaaca gtgaatgtgc agcggcaacc
     1321 cgggccgcaa tggggtcggc caaatttaac aagggtttat tcttttatcc actttctgac
     1381 tacaaagacc atgacggtga ttataaagat catgatatcg attacaagga tgacgatgac
     1441 aagtagtaac tcgaggcggc cgc
//