LOCUS       pOME0186       683 bp    DNA     linear   UNA 04-Mar-2014
DEFINITION  MERS EMC JX8 nsp1; stop added; TAGGED; vector pDEST47
SOURCE      UNC178-2 (MERS EMC JX8 nsp1 TAGGED)
COMMENT     Plasmid synthesized by BioBasic
            Sequence verified via Sanger sequencing through Genewiz
            pOME= plasmid of the Orfeome project
            nsP= non-structural protein
            EMC= isolated in Netherlands
FEATURES             Location/Qualifiers
     Site            join(1..6,1^2)
                     /site_type="restriction site"
                     /note="Name: SalI"
                     /note="Pattern: gtcgac"
                     /note="cut_0_on_positive_strand: 1^2"
                     /note="cut_0_on_negative_strand: 5^6"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=SalI
     Site            1..12
                     /site_type="mutagenized site"
                     /label="5' RE tag"
     Site            join(7..12,11^12)
                     /site_type="restriction site"
                     /note="Name: KpnI"
                     /note="Pattern: ggtacc"
                     /note="cut_0_on_positive_strand: 11^12"
                     /note="cut_0_on_negative_strand: 7^8"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=KpnI
     Site            13..18
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             19..666
                     /cds_type=ORF
                     /note="Length: 648"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     CDS             230..589
                     /cds_type=ORF
                     /note="Length: 360"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     Site            598..663
                     /site_type="mutagenized site"
                     /label="3X FLAG tag"
     Site            664..669
                     /site_type="mutagenized site"
                     /label="double stop"
     Site            join(670..675,670^671)
                     /site_type="restriction site"
                     /note="Name: XhoI"
                     /note="Pattern: ctcgag"
                     /note="cut_0_on_positive_strand: 670^671"
                     /note="cut_0_on_negative_strand: 674^675"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=XhoI
     Site            670..683
                     /site_type="mutagenized site"
                     /label="3' RE tag"
     Site            join(676..683,677^678)
                     /site_type="restriction site"
                     /note="Name: NotI"
                     /note="Pattern: gcggccgc"
                     /note="cut_0_on_positive_strand: 677^678"
                     /note="cut_0_on_negative_strand: 681^682"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=NotI
ORIGIN
        1 gtcgacggta ccgccaccAT GTCTTTCGTG GCTGGTGTGA CCGCGCAAGG TGCGCGCGGT
       61 ACGTATCGAG CAGCGCTCAA CTCTGAAAAA CATCAAGACC ATGTGTCTCT AACTGTGCCA
      121 CTCTGTGGTT CAGGAAACCT GGTTGAAAAA CTTTCACCAT GGTTCATGGA TGGCGAAAAT
      181 GCCTATGAAG TGGTGAAGGC CATGTTACTT AAAAAGGAGC CACTTCTCTA TGTGCCCATC
      241 CGGCTGGCTG GACACACTAG ACACCTCCCA GGTCCTCGTG TGTACCTGGT TGAGAGGCTC
      301 ATTGCTTGTG AAAATCCATT CATGGTTAAC CAATTGGCTT ATAGCTCTAG TGCAAATGGC
      361 AGCCTGGTTG GCACAACTTT GCAGGGCAAG CCTATTGGTA TGTTCTTCCC TTATGACATC
      421 GAACTTGTCA CAGGAAAGCA AAATATTCTC CTGCGCAAGT ATGGCCGTGG TGGTTATCAC
      481 TACACCCCAT TCCACTATGA GCGAGACAAC ACCTCTTGCC CTGAGTGGAT GGACGATTTT
      541 GAGGCGGATC CTAAAGGCAA ATATGCCCAG AATCTGCTTA AGAAGTTGAT TGGCGGTgac
      601 tacaaagacc atgacggtga ttataaagat catgatatcg attacaagga tgacgatgac
      661 aagtagtaac tcgaggcggc cgc
//