LOCUS pOME0188 350 bp DNA linear UNA 04-Mar-2014 DEFINITION MERS EMC JX8 E - TAGGED; vector pDEST47 SOURCE UNC178-4 (MERS EMC JX8 E- TAGGED) COMMENT Plasmid synthesized by BioBasic Sequence verified via Sanger sequencing through Genewiz pOME= plasmid of the Orfeome project EMC= isolated in Netherlands FEATURES Location/Qualifiers Site join(1..6,1^2) /site_type="restriction site" /note="Name: SalI" /note="Pattern: gtcgac" /note="cut_0_on_positive_strand: 1^2" /note="cut_0_on_negative_strand: 5^6" /note="inhibited_by: 5': N6-methyladenosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label=SalI Site 1..12 /site_type="mutagenized site" /label="5' RE tag" Site join(7..12,11^12) /site_type="restriction site" /note="Name: KpnI" /note="Pattern: ggtacc" /note="cut_0_on_positive_strand: 11^12" /note="cut_0_on_negative_strand: 7^8" /note="inhibited_by: 5': N6-methyladenosine" /note="site_type: other" /note="restriction site" /label=KpnI Site 13..18 /site_type="mutagenized site" /label="Kozak sequence" CDS 19..333 /cds_type=ORF /note="Length: 315" /note="Found at strand: positive" /note="Start codon: ATG" Site 265..330 /site_type="mutagenized site" /label="3X FLAG tag" Site 331..336 /site_type="mutagenized site" /label="double stop" Site join(337..342,337^338) /site_type="restriction site" /note="Name: XhoI" /note="Pattern: ctcgag" /note="cut_0_on_positive_strand: 337^338" /note="cut_0_on_negative_strand: 341^342" /note="inhibited_by: 5': N6-methyladenosine" /note="site_type: other" /note="restriction site" /label=XhoI Site 337..350 /site_type="mutagenized site" /label="3' RE tag" Site join(343..350,344^345) /site_type="restriction site" /note="Name: NotI" /note="Pattern: gcggccgc" /note="cut_0_on_positive_strand: 344^345" /note="cut_0_on_negative_strand: 348^349" /note="inhibited_by: 5': N4-methylcytosine" /note="site_type: other" /note="restriction site" /label=NotI ORIGIN 1 gtcgacggta ccgccaccAT GTTACCCTTT GTCCAAGAAC GAATAGGGTT GTTCATAGTA 61 AACTTTTTCA TTTTTACCGT AGTATGTGCT ATAACACTCT TGGTGTGTAT GGCTTTCCTT 121 ACGGCTACTA GATTATGTGT GCAATGTATG ACAGGCTTCA ATACCCTGTT AGTTCAGCCC 181 GCATTATACT TGTATAATAC TGGACGTTCA GTCTATGTAA AATTCCAGGA TAGTAAACCC 241 CCTCTACCAC CTGACGAGTG GGTTgactac aaagaccatg acggtgatta taaagatcat 301 gatatcgatt acaaggatga cgatgacaag tagtaactcg aggcggccgc //