LOCUS       pOME0188       350 bp    DNA     linear   UNA 04-Mar-2014
DEFINITION  MERS EMC JX8 E - TAGGED; vector pDEST47
SOURCE      UNC178-4 (MERS EMC JX8 E- TAGGED)
COMMENT     Plasmid synthesized by BioBasic
            Sequence verified via Sanger sequencing through Genewiz
            pOME= plasmid of the Orfeome project
            EMC= isolated in Netherlands
FEATURES             Location/Qualifiers
     Site            join(1..6,1^2)
                     /site_type="restriction site"
                     /note="Name: SalI"
                     /note="Pattern: gtcgac"
                     /note="cut_0_on_positive_strand: 1^2"
                     /note="cut_0_on_negative_strand: 5^6"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=SalI
     Site            1..12
                     /site_type="mutagenized site"
                     /label="5' RE tag"
     Site            join(7..12,11^12)
                     /site_type="restriction site"
                     /note="Name: KpnI"
                     /note="Pattern: ggtacc"
                     /note="cut_0_on_positive_strand: 11^12"
                     /note="cut_0_on_negative_strand: 7^8"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=KpnI
     Site            13..18
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             19..333
                     /cds_type=ORF
                     /note="Length: 315"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     Site            265..330
                     /site_type="mutagenized site"
                     /label="3X FLAG tag"
     Site            331..336
                     /site_type="mutagenized site"
                     /label="double stop"
     Site            join(337..342,337^338)
                     /site_type="restriction site"
                     /note="Name: XhoI"
                     /note="Pattern: ctcgag"
                     /note="cut_0_on_positive_strand: 337^338"
                     /note="cut_0_on_negative_strand: 341^342"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=XhoI
     Site            337..350
                     /site_type="mutagenized site"
                     /label="3' RE tag"
     Site            join(343..350,344^345)
                     /site_type="restriction site"
                     /note="Name: NotI"
                     /note="Pattern: gcggccgc"
                     /note="cut_0_on_positive_strand: 344^345"
                     /note="cut_0_on_negative_strand: 348^349"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=NotI
ORIGIN
        1 gtcgacggta ccgccaccAT GTTACCCTTT GTCCAAGAAC GAATAGGGTT GTTCATAGTA
       61 AACTTTTTCA TTTTTACCGT AGTATGTGCT ATAACACTCT TGGTGTGTAT GGCTTTCCTT
      121 ACGGCTACTA GATTATGTGT GCAATGTATG ACAGGCTTCA ATACCCTGTT AGTTCAGCCC
      181 GCATTATACT TGTATAATAC TGGACGTTCA GTCTATGTAA AATTCCAGGA TAGTAAACCC
      241 CCTCTACCAC CTGACGAGTG GGTTgactac aaagaccatg acggtgatta taaagatcat
      301 gatatcgatt acaaggatga cgatgacaag tagtaactcg aggcggccgc
//