LOCUS       pOME0206       992 bp    DNA     linear   UNA 04-Sep-2014
DEFINITION  ZEBOV small secreted GP TAGGED; vector pDEST47
COMMENT     Plasmid synthesized by BioBasic
            Sequence verified via Sanger sequencing through Genewiz
            pOME= plasmid of the Orfeome project
            GP= glycoprotein
            ZEBOV= Zaire Ebola Virus
FEATURES             Location/Qualifiers
     Site            join(1..6,5^6)
                     /site_type="restriction site"
                     /note="Name: KpnI"
                     /note="Pattern: ggtacc"
                     /note="cut_0_on_positive_strand: 5^6"
                     /note="cut_0_on_negative_strand: 1^2"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=KpnI
     Site            1..6
                     /site_type="mutagenized site"
                     /label="5' RE tag"
     Site            7..12
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             13..975
                     /cds_type=ORF
                     /note="Length: 963"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     Site            907..972
                     /site_type="mutagenized site"
                     /label="3X FLAG tag"
     Site            973..978
                     /site_type="mutagenized site"
                     /label="double stop"
     Site            join(979..984,979^980)
                     /site_type="restriction site"
                     /note="Name: XhoI"
                     /note="Pattern: ctcgag"
                     /note="cut_0_on_positive_strand: 979^980"
                     /note="cut_0_on_negative_strand: 983^984"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=XhoI
     Site            979..992
                     /site_type="mutagenized site"
                     /label="3' RE tag"
     Site            join(985..992,986^987)
                     /site_type="restriction site"
                     /note="Name: NotI"
                     /note="Pattern: gcggccgc"
                     /note="cut_0_on_positive_strand: 986^987"
                     /note="cut_0_on_negative_strand: 990^991"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=NotI
ORIGIN
        1 ggtaccgcca ccATGGGCGT TACAGGAATA TTGCAGTTAC CTCGTGATCG ATTCAAGAGG
       61 ACATCATTCT TTCTTTGGGT AATTATCCTT TTCCAAAGAA CATTTTCCAT CCCACTTGGA
      121 GTCATCCACA ATAGCACATT ACAGGTTAGT GATGTCGACA AACTAGTTTG TCGTGACAAA
      181 CTGTCATCCA CAAATCAATT GAGATCAGTT GGACTGAATC TCGAAGGGAA TGGAGTGGCA
      241 ACTGACGTGC CATCTGCAAC TAAAAGATGG GGCTTCAGGT CCGGTGTCCC ACCAAAGGTG
      301 GTCAATTATG AAGCTGGTGA ATGGGCTGAA AACTGCTACA ATCTTGAAAT CAAAAAACCT
      361 GACGGGAGTG AGTGTCTACC AGCAGCGCCA GACGGGATTC GGGGCTTCCC CCGGTGCCGG
      421 TATGTGCACA AAGTATCAGG AACGGGACCG TGTGCCGGAG ACTTTGCCTT CCATAAAGAG
      481 GGTGCTTTCT TCCTGTATGA TCGACTTGCT TCCACAGTTA TCTACCGAGG AACGACTTTC
      541 GCTGAAGGTG TCGTTGCATT TCTGATACTG CCCCAAGCTA AGAAGGACTT CTTCAGCTCA
      601 CACCCCTTGA GAGAGCCGGT CAATGCAACG GAGGACCCGT CTAGTGGCTA CTATTCTACC
      661 ACAATTAGAT ATCAGGCTAC CGGTTTTGGA ACCAATGAGA CAGAGTACTT GTTCGAGGTT
      721 GACAATTTGA CCTACGTCCA ACTTGAATCA AGATTCACAC CACAGTTTCT GCTCCAGCTG
      781 AATGAGACAA TATATACAAG TGGGAAAAGG AGCAATACCA CGGGAAAACT AATTTGGAAG
      841 GTCAACCCCG AAATTGATAC AACAATCGGG GAGTGGGCCT TCTGGGAAAC TAAAAAAAAA
      901 CCTCACgact acaaagacca tgacggtgat tataaagatc atgatatcga ttacaaggat
      961 gacgatgaca agtagtaact cgaggcggcc gc
//