LOCUS pOME0206 992 bp DNA linear UNA 04-Sep-2014 DEFINITION ZEBOV small secreted GP TAGGED; vector pDEST47 COMMENT Plasmid synthesized by BioBasic Sequence verified via Sanger sequencing through Genewiz pOME= plasmid of the Orfeome project GP= glycoprotein ZEBOV= Zaire Ebola Virus FEATURES Location/Qualifiers Site join(1..6,5^6) /site_type="restriction site" /note="Name: KpnI" /note="Pattern: ggtacc" /note="cut_0_on_positive_strand: 5^6" /note="cut_0_on_negative_strand: 1^2" /note="inhibited_by: 5': N6-methyladenosine" /note="site_type: other" /note="restriction site" /label=KpnI Site 1..6 /site_type="mutagenized site" /label="5' RE tag" Site 7..12 /site_type="mutagenized site" /label="Kozak sequence" CDS 13..975 /cds_type=ORF /note="Length: 963" /note="Found at strand: positive" /note="Start codon: ATG" Site 907..972 /site_type="mutagenized site" /label="3X FLAG tag" Site 973..978 /site_type="mutagenized site" /label="double stop" Site join(979..984,979^980) /site_type="restriction site" /note="Name: XhoI" /note="Pattern: ctcgag" /note="cut_0_on_positive_strand: 979^980" /note="cut_0_on_negative_strand: 983^984" /note="inhibited_by: 5': N6-methyladenosine" /note="site_type: other" /note="restriction site" /label=XhoI Site 979..992 /site_type="mutagenized site" /label="3' RE tag" Site join(985..992,986^987) /site_type="restriction site" /note="Name: NotI" /note="Pattern: gcggccgc" /note="cut_0_on_positive_strand: 986^987" /note="cut_0_on_negative_strand: 990^991" /note="inhibited_by: 5': N4-methylcytosine" /note="site_type: other" /note="restriction site" /label=NotI ORIGIN 1 ggtaccgcca ccATGGGCGT TACAGGAATA TTGCAGTTAC CTCGTGATCG ATTCAAGAGG 61 ACATCATTCT TTCTTTGGGT AATTATCCTT TTCCAAAGAA CATTTTCCAT CCCACTTGGA 121 GTCATCCACA ATAGCACATT ACAGGTTAGT GATGTCGACA AACTAGTTTG TCGTGACAAA 181 CTGTCATCCA CAAATCAATT GAGATCAGTT GGACTGAATC TCGAAGGGAA TGGAGTGGCA 241 ACTGACGTGC CATCTGCAAC TAAAAGATGG GGCTTCAGGT CCGGTGTCCC ACCAAAGGTG 301 GTCAATTATG AAGCTGGTGA ATGGGCTGAA AACTGCTACA ATCTTGAAAT CAAAAAACCT 361 GACGGGAGTG AGTGTCTACC AGCAGCGCCA GACGGGATTC GGGGCTTCCC CCGGTGCCGG 421 TATGTGCACA AAGTATCAGG AACGGGACCG TGTGCCGGAG ACTTTGCCTT CCATAAAGAG 481 GGTGCTTTCT TCCTGTATGA TCGACTTGCT TCCACAGTTA TCTACCGAGG AACGACTTTC 541 GCTGAAGGTG TCGTTGCATT TCTGATACTG CCCCAAGCTA AGAAGGACTT CTTCAGCTCA 601 CACCCCTTGA GAGAGCCGGT CAATGCAACG GAGGACCCGT CTAGTGGCTA CTATTCTACC 661 ACAATTAGAT ATCAGGCTAC CGGTTTTGGA ACCAATGAGA CAGAGTACTT GTTCGAGGTT 721 GACAATTTGA CCTACGTCCA ACTTGAATCA AGATTCACAC CACAGTTTCT GCTCCAGCTG 781 AATGAGACAA TATATACAAG TGGGAAAAGG AGCAATACCA CGGGAAAACT AATTTGGAAG 841 GTCAACCCCG AAATTGATAC AACAATCGGG GAGTGGGCCT TCTGGGAAAC TAAAAAAAAA 901 CCTCACgact acaaagacca tgacggtgat tataaagatc atgatatcga ttacaaggat 961 gacgatgaca agtagtaact cgaggcggcc gc //