LOCUS pOME0215 440 bp DNA linear UNA 23-Oct-2013 DEFINITION MERS CoV England ORF8B WT tagged nt 287129-29067; received from RG; vector pDEST47 KEYWORDS SOURCE UNC163-18 (MERS CoV England ORF8B WT tagged) COMMENT Plasmid synthesized by BioBasic Sequence verified via Sanger sequencing through Genewiz pOME= plasmid of the Orfeome project WT= wild type FEATURES Location/Qualifiers Site join(1..6,1^2) /site_type="restriction site" /note="Name: SalI" /note="Pattern: gtcgac" /note="cut_0_on_positive_strand: 1^2" /note="cut_0_on_negative_strand: 5^6" /note="inhibited_by: 5': N6-methyladenosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label=SalI Site 1..12 /site_type="mutagenized site" /label="5' RE tag" Site join(7..12,11^12) /site_type="restriction site" /note="Name: KpnI" /note="Pattern: ggtacc" /note="cut_0_on_positive_strand: 11^12" /note="cut_0_on_negative_strand: 7^8" /note="inhibited_by: 5': N6-methyladenosine" /note="site_type: other" /note="restriction site" /label=KpnI Site 13..18 /site_type="mutagenized site" /label="Kozak sequence" CDS 19..423 /cds_type=ORF /note="Length: 405" /note="Found at strand: positive" /note="Start codon: ATG" Site 355..420 /site_type="mutagenized site" /label="3X FLAG tag" Site 421..426 /site_type="mutagenized site" /label="double stop" Site join(427..432,427^428) /site_type="restriction site" /note="Name: XhoI" /note="Pattern: ctcgag" /note="cut_0_on_positive_strand: 427^428" /note="cut_0_on_negative_strand: 431^432" /note="inhibited_by: 5': N6-methyladenosine" /note="site_type: other" /note="restriction site" /label=XhoI Site 427..440 /site_type="mutagenized site" /label="3' RE tag" Site join(433..440,434^435) /site_type="restriction site" /note="Name: NotI" /note="Pattern: gcggccgc" /note="cut_0_on_positive_strand: 434^435" /note="cut_0_on_negative_strand: 438^439" /note="inhibited_by: 5': N4-methylcytosine" /note="site_type: other" /note="restriction site" /label=NotI ORIGIN 1 gtcgacggta ccgccaccAT GCCAATTCCA CCCCTGCGCA AAATGCTGGG TATTGGCGGA 61 GACAGGACAG AAAAATTAAT ACCGGGAATG GAATTAAGCA ACTGGCTCCC AGGTGGTATT 121 TCTACTACAC TGGAACTGGA CCCGAAGCAG CACTCCCATT CCGGGCTGTT AAGGATGGCA 181 TCGTTTGGGT CCATGAAGAT GGCGCCACTG ATGCTCCTTC AACTTTTGGG ACGCGGAACC 241 CTAACAATGA TTCAGCTATT GTTACACAAT TCGCGCCCGG TACTAAGCTT CCTAAAAACT 301 TCCACATTGA GGGGACTGGA GGCAATAGTC AATCATCTTC AAGAGCCTCT AGCGgactac 361 aaagaccatg acggtgatta taaagatcat gatatcgatt acaaggatga cgatgacaag 421 tagtaactcg aggcggccgc //