LOCUS       pOME0223       404 bp    DNA     linear   UNA 11-Dec-2014
DEFINITION  Ca04 PB2 Hypo #1 TAGGED; vector pDEST47
COMMENT     Plasmid synthesized by BioBasic
            Sequence verified via Sanger sequencing through Genewiz
            pOME= plasmid of the Orfeome project
            Ca04 PB2= Influenze type A/ California/ 04/ 2009 H1N1, PB2 gene
            hypo= hypothetical 
FEATURES             Location/Qualifiers
     Site            join(1..6,1^2)
                     /site_type="restriction site"
                     /note="Name: SalI"
                     /note="Pattern: gtcgac"
                     /note="cut_0_on_positive_strand: 1^2"
                     /note="cut_0_on_negative_strand: 5^6"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=SalI
     Site            1..12
                     /site_type="mutagenized site"
                     /label="5' RE tag"
     Site            join(7..12,11^12)
                     /site_type="restriction site"
                     /note="Name: KpnI"
                     /note="Pattern: ggtacc"
                     /note="cut_0_on_positive_strand: 11^12"
                     /note="cut_0_on_negative_strand: 7^8"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=KpnI
     Site            13..18
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             19..390
                     /cds_type=ORF
                     /note="Length: 303"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     Site            319..384
                     /site_type="mutagenized site"
                     /label="3x FLAG tg"
     Site            385..390
                     /site_type="mutagenized site"
                     /label="double stop"
     Site            join(391..396,391^392)
                     /site_type="restriction site"
                     /note="Name: XhoI"
                     /note="Pattern: ctcgag"
                     /note="cut_0_on_positive_strand: 391^392"
                     /note="cut_0_on_negative_strand: 395^396"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=XhoI
     Site            391..404
                     /site_type="mutagenized site"
                     /label="3' RE tag"
     Site            join(397..404,398^399)
                     /site_type="restriction site"
                     /note="Name: NotI"
                     /note="Pattern: gcggccgc"
                     /note="cut_0_on_positive_strand: 398^399"
                     /note="cut_0_on_negative_strand: 402^403"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=NotI
ORIGIN
        1 gtcgacggta ccgccaccAT GGCCATCCGA ATTCTTTTGG TCGCTGTCTG GCTGTCAGTA
       61 AGTATGCTAG AGTCCCGTTT TCGTTTCATT ACCAACACTA CGTCCCCTTG CCCAATTAGC
      121 ACATTAGCCT TCTCTCCTTT TGCAAGATTG CTCAGTTCAT TGATGCTTAA TGCTGGGCCA
      181 TATCTCTTGT CTTCTTTGCC CAAAATGAGA AATCCTCTCA GGACAGCAGA CTCCACCCCA
      241 GATGTGCCTT CATCTGGATC TTCAGTCAAT GCACCTGCAT CCTTTCCAAG AACTGTAAGT
      301 CGTTTGGTTG CCTTGTTGga ctacaaagac catgacggtg attataaaga tcatgatatc
      361 gattacaagg atgacgatga caagtagtaa ctcgaggcgg ccgc
//