LOCUS       pOME0231       455 bp    DNA     linear   UNA 11-Dec-2014
DEFINITION  Mal/NY NP Hypo #1 TAGGED; vector pDEST47
COMMENT     Plasmid synthesized by BioBasic
            Sequence verified via Sanger sequencing through Genewiz
            pOME= plasmid of the Orfeome project
            Mal/NY= Influenza type A/Mallard/NY/6750/78 H2N2
            NP= nuclear protein
            Hypo= hypothetical
FEATURES             Location/Qualifiers
     Site            join(1..6,1^2)
                     /site_type="restriction site"
                     /note="Name: SalI"
                     /note="Pattern: gtcgac"
                     /note="cut_0_on_positive_strand: 1^2"
                     /note="cut_0_on_negative_strand: 5^6"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=SalI
     Site            1..12
                     /site_type="mutagenized site"
                     /label="5' RE tag"
     Site            join(7..12,11^12)
                     /site_type="restriction site"
                     /note="Name: KpnI"
                     /note="Pattern: ggtacc"
                     /note="cut_0_on_positive_strand: 11^12"
                     /note="cut_0_on_negative_strand: 7^8"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=KpnI
     Site            13..18
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             19..441
                     /cds_type=ORF
                     /note="Length: 354"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     Site            370..435
                     /site_type="mutagenized site"
                     /label="3x FLAG tag"
     Site            436..441
                     /site_type="mutagenized site"
                     /label="double stop"
     Site            join(442..447,442^443)
                     /site_type="restriction site"
                     /note="Name: XhoI"
                     /note="Pattern: ctcgag"
                     /note="cut_0_on_positive_strand: 442^443"
                     /note="cut_0_on_negative_strand: 446^447"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=XhoI
     Site            442..455
                     /site_type="mutagenized site"
                     /label="3' RE tag"
     Site            join(448..455,449^450)
                     /site_type="restriction site"
                     /note="Name: NotI"
                     /note="Pattern: gcggccgc"
                     /note="cut_0_on_positive_strand: 449^450"
                     /note="cut_0_on_negative_strand: 453^454"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=NotI
ORIGIN
        1 gtcgacggta ccgccaccAT GTCAAAGGAA GGCACGATCG GGTTCGTCGC CTTTTCGTCC
       61 GAGAGCTCGA AGACTCCCCG CCCCTGGAAA GACACATCTT CAGGTCTGGC ATTTTCCATC
      121 ATCCTTATGA TTTCAGTTCT CATGTCTGAA GTTCTGCCTT CAGTGTTTCC TGTAAATGCT
      181 GCCATAATGG TTGCTCTCTC GAATGGGAGG TTTCTCTGCA CAGAGAATGT GGGCTGTACG
      241 CTGATTTGTC CTGCAGATGC TCTCTGCTGG TTGGTGTTCC CTCCGCTTCT GGTTCTTATA
      301 GCCCAGTATC TGCTTCTCAA TTCAAGAGTA CTGGAGTCCA TTGTCTCCAT GTTCTCATTT
      361 GAAGCAATCg actacaaaga ccatgacggt gattataaag atcatgatat cgattacaag
      421 gatgacgatg acaagtagta actcgaggcg gccgc
//