LOCUS       pOME0250       339 bp    DNA     linear   UNA 22-May-2015
DEFINITION  Ebola VP35-IGR-VP40; vector pSIREN
COMMENT     Plasmid synthesized by BioBasic
            Sequence verified via Sanger sequencing through Genewiz
            pOME= plasmid of the Orfeome project
            VP= viral protein
            IGR= intergenic region 
            the description of VP35-IGR-VP40 means the sequence is of the intergenic region between the viral proteins 35 and 40
FEATURES             Location/Qualifiers
     Site            join(1..6,1^2)
                     /site_type="restriction site"
                     /note="Name: BamHI"
                     /note="Pattern: ggatcc"
                     /note="cut_0_on_positive_strand: 1^2"
                     /note="cut_0_on_negative_strand: 5^6"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=BamHI
     Site            join(334..339,334^335)
                     /site_type="restriction site"
                     /note="Name: EcoRI"
                     /note="Pattern: gaattc"
                     /note="cut_0_on_positive_strand: 334^335"
                     /note="cut_0_on_negative_strand: 338^339"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=EcoRI
ORIGIN
        1 ggatccGCCA ATCTCCCTTC CCTCCGAAAG AGGCGAATAA TAGCAGAGGC TTCAACTGCT
       61 GAACTATAGG GTACGTTACA TTAATGATAC ACTTGTGAGT ATCAGCCCTG GATAATATAA
      121 GTCAATTAAA CGACCAAGAT AAAATTGTTC ATATCTCGCT AGCAGCTTAA AATATAAATG
      181 TAATAGGAGC TATATCTCTG ACAGTATTAT AATCAATTGT TATTAAGTAA CCCAAACCAA
      241 AAGTGATGAA GATTAAGAAA AACCTACCTC GGCTGAGAGA GTGTTTTTTC ATTAACCTTC
      301 ATCTTGTAAA CGTTGAGCAA AATTGTTAAA AATgaattc
//