LOCUS pOME0002L 956 bp DNA linear UNA 14-Oct-2013 DEFINITION KSHV K1 tagged double stop; UNC 154-2 SOURCE UNC154-2 (KSHV K1 tagged) FEATURES Location/Qualifiers Site join(1..6,1^2) /site_type="restriction site" /note="Name: SalI" /note="Pattern: gtcgac" /note="Number_of_matches: 1" /note="cut_0_on_positive_strand: 1^2" /note="cut_0_on_negative_strand: 5^6" /note="inhibited_by: 5': N6-methyladenosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label=SalI Site 1..12 /site_type="mutagenized site" /label="5' RE tag" Site join(7..12,11^12) /site_type="restriction site" /note="Name: KpnI" /note="Pattern: ggtacc" /note="Number_of_matches: 1" /note="cut_0_on_positive_strand: 11^12" /note="cut_0_on_negative_strand: 7^8" /note="inhibited_by: 5': N6-methyladenosine" /note="site_type: other" /note="restriction site" /label=KpnI Site 13..18 /site_type="mutagenized site" /label="Kozak sequence" CDS 19..939 /cds_type=ORF /note="Length: 921" /note="Found at strand: positive" /note="Start codon: atg" Site join(555..560,555^556) /site_type="restriction site" /note="Name: XbaI" /note="Pattern: tctaga" /note="Number_of_matches: 1" /note="cut_0_on_positive_strand: 555^556" /note="cut_0_on_negative_strand: 559^560" /note="inhibited_by: 5': N6-methyladenosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label=XbaI Site 871..943 /site_type="mutagenized site" /label="3x FLAG tag" Site join(907..912,909^910) /site_type="restriction site" /note="Name: EcoRV" /note="Pattern: gatatc" /note="Number_of_matches: 1" /note="cut_0_on_positive_strand: 909^910" /note="cut_0_on_negative_strand: 909^910" /note="inhibited_by: 5': N6-methyladenosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label=EcoRV Site 937..942 /site_type="mutagenized site" /label="Double stop" Site join(943..948,943^944) /site_type="restriction site" /note="Name: XhoI" /note="Pattern: ctcgag" /note="Number_of_matches: 1" /note="cut_0_on_positive_strand: 943^944" /note="cut_0_on_negative_strand: 947^948" /note="inhibited_by: 5': N6-methyladenosine" /note="site_type: other" /note="restriction site" /label=XhoI Site 943..956 /site_type="mutagenized site" /label="3' RE tag" Site join(949..956,950^951) /site_type="restriction site" /note="Name: NotI" /note="Pattern: gcggccgc" /note="Number_of_matches: 1" /note="cut_0_on_positive_strand: 950^951" /note="cut_0_on_negative_strand: 954^955" /note="inhibited_by: 5': N4-methylcytosine" /note="site_type: other" /note="restriction site" /label=NotI ORIGIN 1 gtcgacggta ccgccaccat gttcctgtat gttgtttgca gtctggcggt ttgctttcga 61 ggactattaa gcctttctct gcaatcgtct ccaaatttgt gccctggagt gatttcaacg 121 ccttacacgt tgacctgtcc gtctaataca tccttgccaa catcctggta ttgcaacgat 181 actcggcttc tccgactgac gcagcaaaca atcactgttg tcacccttat ctgcaatttt 241 agttgtgtgg gacaatctgg gcatcgacac agcctttgga ttacatggta tccacaacct 301 gtcttacaaa ccttgtgtgg acaaccatca aacacagtca cttgtggtca gcatgttact 361 ttgtattgtt ctacctctgg aaataatgtt accgtttggc atctaccaaa cggacgaaat 421 gaaaccgtgt cacaaactaa atactataat tttacgctga tggaccaaac ggaggggtgt 481 tatgcttgtt ctgacgggct gtcgtctcgc ctgtcaaatc gtctatgttt ttcggcgcgt 541 tgtgccaata taactctaga aactaatact gtatctgtca gcagtactac aggctttaga 601 acattcagta ctaatagcga tgcaaccaca catgatatac ttgtaatgaa agaagccaaa 661 tctacaaatc tacatattca agtgcatttt cttgtattta tgacactcgt agctctgata 721 ggaaccatgt gtggtatctt aggaactatt atctttgccc attgtcaaaa acaaagtgac 781 tcaaacaaaa cagtgccaca acaattgcgg gattattatt ccctacacga tttgtgcacg 841 gaagactata cgcaaccagt ggattggtac gactacaaag accatgacgg tgattataaa 901 gatcatgata tcgattacaa ggatgacgat gacaagtagt aactcgaggc ggccgc //