LOCUS       pOME0008      1064 bp    DNA     linear   UNA 15-Oct-2013
DEFINITION  KSHV K3; UNC154-8; vector pDEST47
SOURCE      KSHV
  ORGANISM  KSHV
            KSHV K3; UNC154-8; Synthetic Construct.
REFERENCE   1 (bases 1 to 887)
  AUTHORS   Baric,R.S., Corcoran,K., Damania,B., Dittmer,D.P.,
            Graham,R.L., Halfmann,P., Heise,M.T., Kawaoka,Y.,
            Long,K.M., Moorman,N.J., Neumann,G., Sherrod,C.J., and
            Texier,J.R.
  TITLE     Unpublished
COMMENT     Plasmid synthesized by BioBasic
            Sequence verified via Sanger Sequencing through Genewiz
            pOME= plasmid of the Orfeome project 
            only NotI site 3'
FEATURES             Location/Qualifiers
     Site            join(1..6,1^2)
                     /site_type="restriction site"
                     /label=SalI
     Site            1..12
                     /site_type="mutagenized site"
                     /label="5' RE tag"
     Site            join(7..12,11^12)
                     /site_type="restriction site"
                     /label=KpnI
     Site            13..18
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             19..1053
                     /cds_type=ORF
                     /note="Length: 1035"
                     /note="Found at strand: positive"
                     /note="Start codon: atg"
     Site            985..1050
                     /site_type="mutagenized site"
                     /label="3X FLAG tag"
     Site            1051..1056
                     /site_type="mutagenized site"
                     /label="Double stop"
     Site            join(1057..1064,1058^1059)
                     /site_type="restriction site"
                     /label=NotI
     Site            1057..1064
                     /site_type="mutagenized site"
                     /label="3' RE site"
ORIGIN
        1 gtcgacggta ccgccaccat ggaagatgag gatgttcctg tctgctggat ttgcaacgag
       61 gagctcggaa atgagagatt tagagcctgt ggatgcacag gagagctcga gaacgtccat
      121 agaagttgtt taagcacctg gctcactatc tctagaaaca cggcctgtca gatatgtggc
      181 gtcgtataca acacgcgcgt ggtctggcga cccttgcgcg aaatgacgct attgcctcgg
      241 ctgacttacc aggagggtct ggaactgatt gtttttattt tcattatgac attgggggcc
      301 gctggccttg ccgctgcgac gtgggtttgg ctatatatag tgggcggtca tgacccagag
      361 atagatcacg tcgctgcagc cgcgtactac gtttttttcg tgttttacca attgtttgtc
      421 gtttttgggt tgggtgcgtt tttccacatg atgcgccacg tggggcgggc atacgctgct
      481 gtaaacacgc gggttgaagt gtttccatat agacctcggc cgacatcacc agagtgtgca
      541 gtagaggaga tcgagcttca ggaaattctt ccccgtgggg ataaccagga cgaggagggg
      601 cccgcggggg cagctccagg cgaccaagat ggccccgcgg atggcgctcc tgtgcatcgc
      661 gactcagaag aatccgtgga tgaagctgca gggtacaagg aagcgggaga accaacacat
      721 aatgatggac gtgatgacaa tgtagagcca accgcggttg ggtgtgactg taacaacttg
      781 ggcgctgagc ggtatagggc cacttactgt ggcggttatg ttggtgccca gtcgggcgat
      841 ggggcttata gtgtctcctg ccataataag gctggaccct cctctctagt tgatatcctt
      901 ccacagggtt tgcctggggg tggctatggt tccatgggcg tgattaggaa acgttcggct
      961 gtttcgtctg cccttatgtt tcatgactac aaagaccatg acggtgatta taaagatcat
     1021 gatatcgatt acaaggatga cgatgacaag tagtaagcgg ccgc
//