LOCUS pOME0008 1064 bp DNA linear UNA 15-Oct-2013 DEFINITION KSHV K3; UNC154-8; vector pDEST47 SOURCE KSHV ORGANISM KSHV KSHV K3; UNC154-8; Synthetic Construct. REFERENCE 1 (bases 1 to 887) AUTHORS Baric,R.S., Corcoran,K., Damania,B., Dittmer,D.P., Graham,R.L., Halfmann,P., Heise,M.T., Kawaoka,Y., Long,K.M., Moorman,N.J., Neumann,G., Sherrod,C.J., and Texier,J.R. TITLE Unpublished COMMENT Plasmid synthesized by BioBasic Sequence verified via Sanger Sequencing through Genewiz pOME= plasmid of the Orfeome project only NotI site 3' FEATURES Location/Qualifiers Site join(1..6,1^2) /site_type="restriction site" /label=SalI Site 1..12 /site_type="mutagenized site" /label="5' RE tag" Site join(7..12,11^12) /site_type="restriction site" /label=KpnI Site 13..18 /site_type="mutagenized site" /label="Kozak sequence" CDS 19..1053 /cds_type=ORF /note="Length: 1035" /note="Found at strand: positive" /note="Start codon: atg" Site 985..1050 /site_type="mutagenized site" /label="3X FLAG tag" Site 1051..1056 /site_type="mutagenized site" /label="Double stop" Site join(1057..1064,1058^1059) /site_type="restriction site" /label=NotI Site 1057..1064 /site_type="mutagenized site" /label="3' RE site" ORIGIN 1 gtcgacggta ccgccaccat ggaagatgag gatgttcctg tctgctggat ttgcaacgag 61 gagctcggaa atgagagatt tagagcctgt ggatgcacag gagagctcga gaacgtccat 121 agaagttgtt taagcacctg gctcactatc tctagaaaca cggcctgtca gatatgtggc 181 gtcgtataca acacgcgcgt ggtctggcga cccttgcgcg aaatgacgct attgcctcgg 241 ctgacttacc aggagggtct ggaactgatt gtttttattt tcattatgac attgggggcc 301 gctggccttg ccgctgcgac gtgggtttgg ctatatatag tgggcggtca tgacccagag 361 atagatcacg tcgctgcagc cgcgtactac gtttttttcg tgttttacca attgtttgtc 421 gtttttgggt tgggtgcgtt tttccacatg atgcgccacg tggggcgggc atacgctgct 481 gtaaacacgc gggttgaagt gtttccatat agacctcggc cgacatcacc agagtgtgca 541 gtagaggaga tcgagcttca ggaaattctt ccccgtgggg ataaccagga cgaggagggg 601 cccgcggggg cagctccagg cgaccaagat ggccccgcgg atggcgctcc tgtgcatcgc 661 gactcagaag aatccgtgga tgaagctgca gggtacaagg aagcgggaga accaacacat 721 aatgatggac gtgatgacaa tgtagagcca accgcggttg ggtgtgactg taacaacttg 781 ggcgctgagc ggtatagggc cacttactgt ggcggttatg ttggtgccca gtcgggcgat 841 ggggcttata gtgtctcctg ccataataag gctggaccct cctctctagt tgatatcctt 901 ccacagggtt tgcctggggg tggctatggt tccatgggcg tgattaggaa acgttcggct 961 gtttcgtctg cccttatgtt tcatgactac aaagaccatg acggtgatta taaagatcat 1021 gatatcgatt acaaggatga cgatgacaag tagtaagcgg ccgc //