LOCUS       pOME0016L      1319 bp    DNA     linear   UNA 17-Oct-2013
DEFINITION  KSHV ORF45 tagged; UNC154-16; BamHI at 5' end for cloning
SOURCE      UNC154-16 (KSHV ORF45 tagged)
FEATURES             Location/Qualifiers
     Site            1..6
                     /site_type="restriction site"
                     /label=BamHI
     Site            1..6
                     /site_type="mutagenized site"
                     /label="5' RE tag"
     Site            7..12
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             13..1302
                     /cds_type=ORF
                     /note="Length: 1290"
                     /note="Found at strand: positive"
                     /note="Start codon: atg"
     Site            1234..1299
                     /site_type="mutagenized site"
                     /label="3X FLAG tag"
     Site            1300..1305
                     /site_type="mutagenized site"
                     /label="double stop"
     Site            1306..1311
                     /site_type="restriction site"
                     /label=XhoI
     Site            1306..1319
                     /site_type="mutagenized site"
                     /label="3' RE tag"
     Site            1312..1319
                     /site_type="restriction site"
                     /label=NotI
ORIGIN
        1 ggatccgcca ccatggcgat gtttgtgagg acctcgtcta gcacacacga tgaagagaga
       61 atgcttccaa ttgaaggagc gcctcgcaga cgaccccccg tgaagttcat attcccacct
      121 ccacctcttt catcacttcc aggatttggc aggccgcgcg gctatgctgg acccacggtg
      181 atagatatgt ctgccccaga cgacgtcttc gccgaggaca cgccatcgcc gccagcaacc
      241 cctctggatc tacagatatc cccggatcag tcgagcggcg aatctgaata tgacgaggat
      301 gaggaagatg aagatgaaga agaaaatgac gatgttcagg aggaagacga gccagagggg
      361 taccctgcag acttttttca acctttatct cacttgcgcc cgaggcctct ggccagacgg
      421 gcccatacgc ccaaaccggt agcagtggta gcgggccgcg tgcgcagttc aacggacacg
      481 gcggagtccg aggcgtccat gggatgggtt agtcaggatg acggattttc ccctgctggg
      541 ctctcacctt cagacgacga gggggttgct atcctggaac cgatggcggc atacactggg
      601 accggggcat acggactttc acctgcttcc agaaatagtg tacctggaac acaaagttca
      661 ccatacagcg accctgatga agggccctcg tggcgccccc tgcgcgccgc acccaccgcg
      721 atcgtcgacc tgacatcgga ctctgatagc gatgacagtt ccaactctcc ggacgtgaac
      781 aatgaggccg cgtttaccga cgcgcgccat ttttcccacc agccaccctc gtccgaggag
      841 gacggagaag accaagggga agtattgagt cagagaatcg ggctcatgga cgtgggccag
      901 aagcgcaaaa ggcagtctac cgcctcctct ggtagcgagg atgtggtgcg ctgccagaga
      961 caaccaaact taagccgcaa agcagtggcg tccgtgataa ttatatcctc ggggagtgac
     1021 acagacgagg agccctcgtc cgccgtgagc gtgatcgtgt ctccgtcgag cacaaagggt
     1081 cacctcccaa cccaatctcc cagtacttcc gcccactcga tttcatcagg aagcacaact
     1141 accgcggggt ccaggtgcag cgacccaacc cgcatcctgg cctccacgcc acccctgtgt
     1201 ggaaacggtg catataactg gccgtggctg gacgactaca aagaccatga cggtgattat
     1261 aaagatcatg atatcgatta caaggatgac gatgacaagt agtaactcga ggcggccgc
//