LOCUS       pOME0024L       461 bp    DNA     linear   UNA 18-Oct-2013
DEFINITION  HKU4 NS3b ORF4a tagged
SOURCE      UNC163-20 (HKU4 NS3b ORF4a tagged)
FEATURES             Location/Qualifiers
     Site            1..6
                     /site_type="restriction site"
                     /label=SalI
     Site            1..12
                     /site_type="mutagenized site"
                     /label="5' RE site"
     Site            7..12
                     /site_type="restriction site"
                     /label=KpnI
     Site            13..18
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             19..444
                     /cds_type=ORF
                     /note="Length: 426"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     Site            376..441
                     /site_type="mutagenized site"
                     /label="3x FLAG tag"
     Site            442..447
                     /site_type="mutagenized site"
                     /label="Double stop"
     Site            448..453
                     /site_type="restriction site"
                     /label=XhoI
     Site            448..461
                     /site_type="mutagenized site"
                     /label="3' RE site"
     Site            454..461
                     /site_type="restriction site"
                     /label=NotI
ORIGIN
        1 gtcgacggta ccgccaccAT GGACTACGTC TCTTTGCTTA ACCAATTTTG GCAGAAGCAA
       61 ATTAAGTCTT ATAAAGAGAC TCCTAGTCAG TATCATTACC TGTATCCACC CAGGTTTTTC
      121 TATAAACCTG TTTTGGGTAA TTTACAGCAC CCTACCAAGT GGTGTTGTAC TATTAAATTT
      181 TATGAGTATA GTGCTCAGGC TACTGAGTGT ACTAAAGCAT CAGCAAAACA AGATGCAGCT
      241 AGACTTATCT GTGAACAGTT ACAAGCTGCT GGACTACTCA ATGGAATGGA GCTCCGATTC
      301 CGTAGTTCTG CCTCCGACAT CTTCGGACAA AACCGTTATG ATGCCAGCAA AAGCTACTTC
      361 TTCTCGAAAA CGGCAgacta caaagaccat gacggtgatt ataaagatca tgatatcgat
      421 tacaaggatg acgatgacaa gtagtaactc gaggcggccg c
//