LOCUS       pOME0026R       953 bp    DNA     linear   UNA 18-Oct-2013
DEFINITION  HKU4 NS3C ORF4b tagged
SOURCE      UNC163-4 (HKU4 NS3c ORF4b tagged)
COMMENT     Only NotI site at 3' end due to internal XhoI
FEATURES             Location/Qualifiers
     Site            1..6
                     /site_type="restriction site"
                     /label=SalI
     Site            1..12
                     /site_type="mutagenized site"
                     /label="5' RE tag"
     Site            7..12
                     /site_type="restriction site"
                     /label=KpnI
     Site            13..18
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             19..942
                     /cds_type=ORF
                     /note="Length: 924"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     CDS             19..873
                     /cds_type=ORF
                     /label=ORF4b
     Site            874..939
                     /site_type="mutagenized site"
                     /label="3x FLAG tag"
     Site            940..945
                     /site_type="mutagenized site"
                     /label="Double stop"
     Site            946..953
                     /site_type="restriction site"
                     /label=NotI
     Site            946..953
                     /site_type="mutagenized site"
                     /label="3' RE tag"
ORIGIN
        1 gtcgacggta ccgccaccAT GAGTATAGTG CTCAGGCTAC TGAGTGTACT AAAGCATCAG
       61 CAAAACAAGA TGCAGCTAGA CTTATCTGTG AACAGTTACA AGCTGCTGGA CTACTCAATG
      121 GAATGGAGCT CCGATTCCGT AGTTCTGCCT CCGACATCTT CGGACAAAAC CGTTATGATG
      181 CCAGCAAAAG CTACTTCTTC TCGAAAACGG CATAGAAAAC CTAAATTGCA ATATGCCAAG
      241 AGAAGGTTTT CCCCTGTTAA TCCAAATGAC TTAGTGCTAG TTCAGCAAGA ACCTACTCAC
      301 TGTGTTCGTT TGGTTTTTCC TTTAAGTAAG CGCTGGATAC ATTTTGATGG ACTCGTCTAT
      361 TCTTTAGCGC GTCTTAATGT GTCTATGACT GTTGATTATC ATGTTACCCT AGCTCTCATC
      421 TATGCTCCTG AGGCTGGTGA ATGCTTTGGT AACTTTTTAC ATCTTTGTCC ATTGCTTAAA
      481 GATTGCTTGT TGGAGTTTAA AAAGCTCTGT GTTCTTGGCA AAACACTTAC CATTTTGGCA
      541 AGTGAATGGC CATTTTTTAC AGATGTTAAA AAGAATAAAG ACAATTTAAC TGTTCCTAAA
      601 GCTGTAGAGT GGCTAAAGGA ACACGGCTAT GAGATATATA ATTCTCAATT GCCTTTACAT
      661 ATGTCTTTAG CAAAATTACA TGACTTACCA CAAGCACAGT TTGCTGAAGC GGCTGGTTTA
      721 TGTCATTATT TTGATCCTCG AGAGTTTGCT TTACCTTGCG CTCTTGAAGT AGTTAAGATA
      781 GGTGGTGGTA AAGTTAATGG TAGATCCATT CCACTTGCCC GCTTCCCAAT TAATAATGAG
      841 TTTAAATTCA TTCCTTATCT TTATCAATGT GTTgactaca aagaccatga cggtgattat
      901 aaagatcatg atatcgatta caaggatgac gatgacaagt agtaagcggc cgc
//