LOCUS       pOME0028       779 bp    DNA     linear   UNA 18-Oct-2013
DEFINITION  HKU4 NS3D ORF5 tagged
SOURCE      UNC163-6
             (HKU4 NS3D ORF 5 tagged)
COMMENT     5' end has no SalI site due to internal site created with addition of FLAG tag
FEATURES             Location/Qualifiers
     Site            1..6
                     /site_type="restriction site"
                     /label=KpnI
     Site            1..6
                     /site_type="mutagenized site"
                     /label="5' RE tag"
     Site            7..12
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             13..693
                     /cds_type=ORF
                     /label="ORF 5"
     CDS             13..762
                     /cds_type=ORF
                     /note="Length: 750"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     Site            694..759
                     /site_type="mutagenized site"
                     /label="3X FLAG tag"
     Site            760..765
                     /site_type="mutagenized site"
                     /label="double stop"
     Site            766..771
                     /site_type="restriction site"
                     /label=XhoI
     Site            772..779
                     /site_type="restriction site"
                     /label=NotI
ORIGIN
        1 ggtaccgcca ccATGGCTTT TTCAGCTTCT TTATTTCGGA CTAAAACAGT CCATACTGAA
       61 GATGCATTTT GCCCTCGTTC GGCTATTCAG GCTGAACAGC CTCCTAATAT CATAGACTGC
      121 ATACCAGTTG CAGGCTATGA AGCTGCATTA ATCACTAATG CACTTTTCCT ATTAGTATTA
      181 TTTGTCTTTA ACCCACTTAC CTGTAAGGGC AATTGGATTA AAGCTATTTT GTTTTACAGC
      241 TTATTGCTGT ATAATATGAT ATTAGCCATA TTTTTGGTTG TTGATACACA ACATTTTGTT
      301 AGTGCTTTGT TGCTAGCTTA TGTTGTTACT TTTCTAGTCT TGTGGACTGC TGACCGAATA
      361 CGTCTCAGTT GTGCAGTAGG AAGTGTATTA CCCTTTGTCG ATATGCGGTC TTCCTATATC
      421 CGTGTTGACA ACGGTAATTC CAGTGTTGTA GTTCCAATGA ACCACACAAA GCACTGGTTT
      481 ATAAGAAATT TTGAGCAATC TTGTCATTGT GAAAATTGTT TTTATATTCA CTCTTCATCT
      541 TATGTTGAGT GTACTTTTAT TAGTCGCCTA AAGAAATCCA TACTTGTTTC AGTTTGTGAT
      601 TTTTCTTTAG GTGGTAATGT TTCTACTGTT TTTGTCCCTT CTAGTGATAA AACAGTACCC
      661 CTTCACATAA TCGCACCTAG CAAGCTTTAT GTCgactaca aagaccatga cggtgattat
      721 aaagatcatg atatcgatta caaggatgac gatgacaagt agtaactcga ggcggccgc
//