LOCUS       pOME0032       461 bp    DNA     linear   UNA 22-Oct-2013
DEFINITION  HKU5 ORF4A
FEATURES             Location/Qualifiers
     Site            1..6
                     /site_type="restriction site"
                     /label=SalI
     Site            1..12
                     /site_type="mutagenized site"
                     /label="5' RE tag"
     Site            7..12
                     /site_type="restriction site"
                     /label=KpnI
     Site            13..18
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             19..444
                     /cds_type=ORF
                     /note="Length: 426"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     Site            376..441
                     /site_type="mutagenized site"
                     /label="3X FLAG tag"
     Site            442..447
                     /site_type="mutagenized site"
                     /label="double stop"
     Site            448..453
                     /site_type="restriction site"
                     /label=XhoI
     Site            448..461
                     /site_type="mutagenized site"
                     /label="3' RE tag"
     Site            454..461
                     /site_type="restriction site"
                     /label=NotI
ORIGIN
        1 gtcgacggta ccgccaccAT GGATTACGTG TCGCTGCTCA ACCAGGTTTG GCAGAAACAG
       61 GTTAATTCCT CTCAAGAAGG AACTTTGGCT CCTGTTCGCC CTACATACGC CTATCGGCCA
      121 GTGCAGGGAA ATCTTCAGTG TCCTATCAAG TGGCGTTGCA TTTACACTTT TGCAGGCTAC
      181 ACAGGTACTG CAACTGAGCC AACTAAAGTA TTAGCAAAAC AAGAAGCCGC TAGGAAGGTT
      241 TGCCTTAGGC TTCAAGAGTA TGGACGACTC GATGGATTTG GACTTAGACT GCGTTATAGC
      301 ACAGCCTTCG AGCACAATCG TTATGATGCC TCTAAGTCCT ATTTCTACAC GCAAACGTCG
      361 TCGTCATCCC ATGAAgacta caaagaccat gacggtgatt ataaagatca tgatatcgat
      421 tacaaggatg acgatgacaa gtagtaactc gaggcggccg c
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