LOCUS pOME0044L 470 bp DNA linear UNA 23-Oct-2013 DEFINITION SARS ORF7a nt 27273-27641 tagged SOURCE UNC160-4 (SARS Orf7a tagged) FEATURES Location/Qualifiers Site 1..6 /site_type="restriction site" /label=SalI Site 1..12 /site_type="mutagenized site" /label="5' RE tag" Site 7..12 /site_type="restriction site" /label=KpnI Site 13..18 /site_type="mutagenized site" /label="Kozak sequence" CDS 19..453 /cds_type=ORF /note="Length: 435" /note="Found at strand: positive" /note="Start codon: ATG" Site 385..450 /site_type="mutagenized site" /label="3X FLAG tag" Site 451..456 /site_type="mutagenized site" /label="double stop" Site 457..462 /site_type="restriction site" /label=XhoI Site 457..470 /site_type="mutagenized site" /label="3' RE tag" Site 463..470 /site_type="restriction site" /label=NotI ORIGIN 1 gtcgacggta ccgccaccAT GAAAATTATT CTCTTCCTGA CATTGATTGT ATTTACATCT 61 TGCGAGCTAT ATCACTATCA GGAGTGTGTT AGAGGTACGA CTGTACTACT AAAAGAACCT 121 TGCCCATCAG GAACATACGA GGGCAATTCA CCATTTCACC CTCTTGCTGA CAATAAATTT 181 GCACTAACTT GCACTAGCAC ACACTTTGCT TTTGCTTGTG CTGACGGTAC TCGACATACC 241 TATCAGCTGC GTGCAAGATC AGTTTCACCA AAACTTTTCA TCAGACAAGA GGAGGTTCAA 301 CAAGAGCTCT ACTCGCCACT TTTTCTCATT GTTGCTGCTC TAGTATTTTT AATACTTTGC 361 TTCACCATTA AGAGAAAGAC AGAAgactac aaagaccatg acggtgatta taaagatcat 421 gatatcgatt acaaggatga cgatgacaag tagtaactcg aggcggccgc //