LOCUS       pOME0044L       470 bp    DNA     linear   UNA 23-Oct-2013
DEFINITION  SARS ORF7a nt 27273-27641 tagged
SOURCE      UNC160-4 (SARS Orf7a tagged)
FEATURES             Location/Qualifiers
     Site            1..6
                     /site_type="restriction site"
                     /label=SalI
     Site            1..12
                     /site_type="mutagenized site"
                     /label="5' RE tag"
     Site            7..12
                     /site_type="restriction site"
                     /label=KpnI
     Site            13..18
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             19..453
                     /cds_type=ORF
                     /note="Length: 435"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     Site            385..450
                     /site_type="mutagenized site"
                     /label="3X FLAG tag"
     Site            451..456
                     /site_type="mutagenized site"
                     /label="double stop"
     Site            457..462
                     /site_type="restriction site"
                     /label=XhoI
     Site            457..470
                     /site_type="mutagenized site"
                     /label="3' RE tag"
     Site            463..470
                     /site_type="restriction site"
                     /label=NotI
ORIGIN
        1 gtcgacggta ccgccaccAT GAAAATTATT CTCTTCCTGA CATTGATTGT ATTTACATCT
       61 TGCGAGCTAT ATCACTATCA GGAGTGTGTT AGAGGTACGA CTGTACTACT AAAAGAACCT
      121 TGCCCATCAG GAACATACGA GGGCAATTCA CCATTTCACC CTCTTGCTGA CAATAAATTT
      181 GCACTAACTT GCACTAGCAC ACACTTTGCT TTTGCTTGTG CTGACGGTAC TCGACATACC
      241 TATCAGCTGC GTGCAAGATC AGTTTCACCA AAACTTTTCA TCAGACAAGA GGAGGTTCAA
      301 CAAGAGCTCT ACTCGCCACT TTTTCTCATT GTTGCTGCTC TAGTATTTTT AATACTTTGC
      361 TTCACCATTA AGAGAAAGAC AGAAgactac aaagaccatg acggtgatta taaagatcat
      421 gatatcgatt acaaggatga cgatgacaag tagtaactcg aggcggccgc
//