LOCUS       pOME0060L       470 bp    DNA     linear   UNA 23-Oct-2013
DEFINITION  HKU3 ORF 7a nt 27217-27585 received from RG tagged
SOURCE      UNC160-16 (HKU3 ORF 7a tagged)
FEATURES             Location/Qualifiers
     Site            1..6
                     /site_type="restriction site"
                     /label=SalI
     Site            1..12
                     /site_type="mutagenized site"
                     /label="5' RE tag"
     Site            7..12
                     /site_type="restriction site"
                     /label=KpnI
     Site            13..18
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             19..453
                     /cds_type=ORF
                     /note="Length: 435"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     Site            386..450
                     /site_type="mutagenized site"
                     /label="3X FLAG tag"
     Site            451..456
                     /site_type="mutagenized site"
                     /label="double stop"
     Site            457..462
                     /site_type="restriction site"
                     /label=XhoI
     Site            457..470
                     /site_type="mutagenized site"
                     /label="3' RE tag"
     Site            463..470
                     /site_type="restriction site"
                     /label=NotI
ORIGIN
        1 gtcgacggta ccgccaccAT GAAAATTATT CTCTTCTTGA CATTGATAGC GCTTGCAACT
       61 TGTGAGTTAT ATCATTATCA GGAGTGTGTT AGGGGTACGA CTGTACTACT AAAAGAACCT
      121 TGCCCATCAG GAACATATGA GGGCAATTCA CCATTTCACC CTCTTGCTGA TAATAAATTT
      181 GCACTAACTT GCTCTAGCAC ACATTTTGCT TTTGCTTGTG CTGACGGTAC TAGACACACC
      241 TATCAGCTTC GTGCAAGATC AGTTTCACCA AAACTTTTCA TCAGACAAGA GGAAGTTTAT
      301 CAAGAGCTCT ACTCACCACT TTTCCTCATT GTTGCTGCAT TAGTATTTAT AATACTTTGC
      361 TTCACCATTA AGAGAAAGAC AGAAgactac aaagaccatg acggtgatta taaagatcat
      421 gatatcgatt acaaggatga cgatgacaag tagtaactcg aggcggccgc
//