LOCUS       pOME0078       431 bp    DNA     linear   UNA 23-Oct-2013
DEFINITION  MERS CoV EMC ORF4a tagged nt 25852-26181; received from RG
FEATURES             Location/Qualifiers
     Site            1..6
                     /site_type="restriction site"
                     /label=SalI
     Site            1..12
                     /site_type="mutagenized site"
                     /label="5' RE tag"
     Site            7..12
                     /site_type="restriction site"
                     /label=KpnI
     Site            13..18
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             19..414
                     /cds_type=ORF
                     /note="Length: 396"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     Site            346..411
                     /site_type="mutagenized site"
                     /label="3X FLAG tag"
     Site            412..417
                     /site_type="mutagenized site"
                     /label="double stop"
     Site            418..423
                     /site_type="restriction site"
                     /label=XhoI
     Site            418..431
                     /site_type="mutagenized site"
                     /label="3' RE tag"
     Site            424..431
                     /site_type="restriction site"
                     /label=NotI
ORIGIN
        1 gtcgacggta ccgccaccAT GGATTACGTG TCTCTGCTTA ATCAAATTTG GCAGAAGTAC
       61 CTTAACTCAC CGTATACTAC TTGTTTGTAC ATCCCTAAAC CCACAGCTAA GTATACACCT
      121 TTAGTTGGCA CTTCATTGCA CCCTGTGCTG TGGAACTGTC AGCTATCCTT TGCTGGTTAT
      181 ACTGAATCTG CTGTTAATTC TACAAAAGCT TTGGCCAAAC AGGACGCAGC TCAGCGAATC
      241 GCTTGGTTGC TACATAAGGA TGGAGGAATC CCTGATGGAT GTTCCCTCTA CCTCCGGCAC
      301 TCAAGTTTAT TCGCGCAAAG CGAGGAAGAG GAGCCATTCT CCAACgacta caaagaccat
      361 gacggtgatt ataaagatca tgatatcgat tacaaggatg acgatgacaa gtagtaactc
      421 gaggcggccg c
//