LOCUS       pOME0088R       842 bp    DNA     linear   UNA 23-Oct-2013
DEFINITION  MERS CoV England ORF4a tagged nt 26092-26832; recieved from RG
SOURCE      UNC163-14 (MERS CoV England ORF4a tagged)
FEATURES             Location/Qualifiers
     Site            1..6
                     /site_type="restriction site"
                     /label=SalI
     Site            1..12
                     /site_type="mutagenized site"
                     /label="5' RE tag"
     Site            7..12
                     /site_type="restriction site"
                     /label=KpnI
     Site            13..18
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             19..825
                     /cds_type=ORF
                     /note="Length: 807"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     CDS             19..756
                     /cds_type=ORF
                     /label=ORF4b
     Site            757..822
                     /site_type="mutagenized site"
                     /label="3X FLAG tag"
     Site            823..828
                     /site_type="mutagenized site"
                     /label="double stop"
     Site            829..834
                     /site_type="restriction site"
                     /label=XhoI
     Site            829..842
                     /site_type="mutagenized site"
                     /label="3' RE tag"
     Site            835..842
                     /site_type="restriction site"
                     /label=NotI
ORIGIN
        1 gtcgacggta ccgccaccAT GGAGGAATCC CTGATGGATG TTCCCTCTAC CTCCGGCACT
       61 CAAGTTTATT CGCGCAAAGC GAGGAAGAGG AGTCATTCTC CAACTAAGAA ACTGCGCTAC
      121 GTTAAGCGTA GATTTTCTCT TCTGCGCCCT GAAGACCTTA GTGTTATTGT CCAACCAACA
      181 CACTATGTCA GGGTTACATT TTCAGACCCC AACATGTGGT ATCTACGTTC GGGTCATCAT
      241 TTACACTCAG TTCACAATTG GCTTAAACCT TATGGCGGCC AACCTGTTTC TGAGTACCAT
      301 ATTACTCTAG CTTTGCTAAA TCTCACTGAT GAAGATTTAG CTAGAGATTT TTCACCCATT
      361 GCGCTCTTTT TGCGCAATGT CAGATTTGAG CTACATGAGT TCGCCTTGCT GCGCAAAACT
      421 CTTGTTCTTA ATGCATCAGA GATCTACTGT GCTAACATAC ATAGATTTAA GCCTGTGTAT
      481 AGAGTTAACA CGGCAATCCC TACTATTAAG GATTGGCTTC TCGTTCAGGG ATTTTCCCTT
      541 TACCATAGTG GCCTCCCTTT ACATATGTCA ATCTCTAAAT TGCATGCACT GGATGATGTT
      601 ACTCGCAATT ACATCATTAC AATGCCATGC TTTAGAACTT ATCCTCAACA AATGTTTGTT
      661 ACTCCTTTGG CCGTAGATGT TGTCTCCATA CGGTCTTCCA ATCAGGGTAA TAAACAAATT
      721 GTTCATTCTT ACCCCATTTT ACATCATCCA GGATTTgact acaaagacca tgacggtgat
      781 tataaagatc atgatatcga ttacaaggat gacgatgaca agtagtaact cgaggcggcc
      841 gc
//