LOCUS       pOME0090       776 bp    DNA     linear   UNA 23-Oct-2013
DEFINITION  MERS CoV England ORF4b tagged nt 26839-27513; received from RG
SOURCE      UNC163-16 (MERS CoV England tagged ORF4b)
FEATURES             Location/Qualifiers
     Site            1..6
                     /site_type="restriction site"
                     /label=SalI
     Site            1..12
                     /site_type="mutagenized site"
                     /label="5' RE tag"
     Site            7..12
                     /site_type="restriction site"
                     /label=KpnI
     Site            13..18
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             19..759
                     /cds_type=ORF
                     /note="Length: 741"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     Site            691..756
                     /site_type="mutagenized site"
                     /label="3X FLAG tag"
     Site            757..762
                     /site_type="mutagenized site"
                     /label="double stop"
     Site            763..768
                     /site_type="restriction site"
                     /label=XhoI
     Site            763..776
                     /site_type="mutagenized site"
                     /label="3' RE tag"
     Site            769..776
                     /site_type="restriction site"
                     /label=NotI
ORIGIN
        1 gtcgacggta ccgccaccAT GGCTTTCTCG GCGTCTTTAT TTAAACCCGT CCAGCTAGTC
       61 CCAGTTTCTC CTGCATTTCA TCGCATTGAG TCTACTGACT CTATTGTTTT CACATACATT
      121 CCTGCTAGCG GCTATGTAGC TGCTTTAGCT GTCAATGTGT GTCTCATTCC CCTATTATTA
      181 CTGCTACGTC AAGATACTTG TCGTCGCAGC ATTATCAGAA CTATGGTTCT CTATTTCCTT
      241 GTTCTGTATA ACTTTTTATT AGCCATTGTA CTAGTCAATG GTGTACATTA TCCAACTGGA
      301 AGTTGCCTGA TAGCCTTCTT AGTTATCCTC ATAATACTTT GGTTTGTAGA TAGAATTCGT
      361 TTCTGTCTCA TGCTGAATTC CTACATTCCA CTGTTTGACA TGCGTTCTCA CTTTATTCGT
      421 GTTAGTACAG TTTCTTCTCA TGGTATGGTC CCTGTCATAC ACACCAAACC ATTATTTATT
      481 AGAAACTTCG ATCAGCGTTG CAGCTGTTCT CGTTGTTTTT ATTTGCACTC TTCCACTTAT
      541 ATAGAGTGCA CTTATATTAG CCGTTTTAGT AAGATTAGCC TAGTTTCTGT AACTGACTTC
      601 TCCTTAAACG GCAATGTTTC CACTGTTTTC GTGCCTGCAA CGCGCGATTC AGTTCCTCTT
      661 CACATAATCG CCCCGAGCTC GCTTATCGTT gactacaaag accatgacgg tgattataaa
      721 gatcatgata tcgattacaa ggatgacgat gacaagtagt aactcgaggc ggccgc
//