LOCUS pOME0099L 437 bp DNA linear UNA 14-Nov-2013 DEFINITION ZEBOV GP Hypo #1 tagged SOURCE UNC 161-6 (ZEBOV GP Hypo #1 tagged) FEATURES Location/Qualifiers Site 1..6 /site_type="restriction site" /label=SalI Site 1..12 /site_type="mutagenized site" /label="5' RE tag" Site 7..12 /site_type="restriction site" /label=KpnI Site 13..18 /site_type="mutagenized site" /label="Kozak sequence" CDS 19..420 /cds_type=ORF /note="Length: 402" /note="Found at strand: positive" /note="Start codon: ATG" Site 352..417 /site_type="mutagenized site" /label="3X FLAG tag" Site 418..423 /site_type="mutagenized site" /label="double stop" Site 424..429 /site_type="restriction site" /label=XhoI Site 424..437 /site_type="mutagenized site" /label="3' RE tag" Site 430..437 /site_type="restriction site" /label=NotI ORIGIN 1 gtcgacggta ccgccaccAT GGAGTGGCAA CTGACGTGCC ATCTGCAACT AAAAGATGGG 61 GCTTCAGGTC CGGTGTCCCA CCAAAGGTGG TCAATTATGA AGCTGGTGAA TGGGCTGAAA 121 ACTGCTACAA TCTTGAAATC AAAAAACCTG ACGGGAGTGA GTGTCTACCA GCAGCGCCAG 181 ACGGGATTCG GGGCTTCCCC CGGTGCCGGT ATGTGCACAA AGTATCAGGA ACGGGACCGT 241 GTGCCGGAGA CTTTGCCTTC CATAAAGAGG GTGCTTTCTT CCTGTATGAT CGACTTGCTT 301 CCACAGTTAT CTACCGAGGA ACGACTTTCG CTGAAGGTGT CGTTGCATTT Cgactacaaa 361 gaccatgacg gtgattataa agatcatgat atcgattaca aggatgacga tgacaagtag 421 taactcgagg cggccgc //