LOCUS       pOME0102R       239 bp    DNA     linear   UNA 14-Nov-2013
DEFINITION  A/Brevig Mission 1/1918 M3; vector pVR21
SOURCE      UNC161-9 (A/Brevig Mission 1/1918 M3)
COMMENT     Plasmid synthesized by BioBasic
            Sequence verified via Sanger sequencing through Genewiz
            pOME= plasmid of the Orfeome project
            A/Brevig Mission/1/1918 M3= Influenza type A/Brevig Mission/isolated in 1918/M3 gene
            R in plasmid name= in replicon vector pVR21
FEATURES             Location/Qualifiers
     Site            join(1..6,1^2)
                     /site_type="restriction site"
                     /note="Name: SalI"
                     /note="Pattern: gtcgac"
                     /note="cut_0_on_positive_strand: 1^2"
                     /note="cut_0_on_negative_strand: 5^6"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=SalI
     Site            1..12
                     /site_type="mutagenized site"
                     /label="5' RE tag"
     Site            join(7..12,11^12)
                     /site_type="restriction site"
                     /note="Name: KpnI"
                     /note="Pattern: ggtacc"
                     /note="cut_0_on_positive_strand: 11^12"
                     /note="cut_0_on_negative_strand: 7^8"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=KpnI
     Site            13..18
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             19..225
                     /cds_type=ORF
                     /note="Length: 207"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     Site            join(226..231,226^227)
                     /site_type="restriction site"
                     /note="Name: XhoI"
                     /note="Pattern: ctcgag"
                     /note="cut_0_on_positive_strand: 226^227"
                     /note="cut_0_on_negative_strand: 230^231"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=XhoI
     Site            226..239
                     /site_type="mutagenized site"
                     /label="3' RE tag"
     Site            join(232..239,233^234)
                     /site_type="restriction site"
                     /note="Name: NotI"
                     /note="Pattern: gcggccgc"
                     /note="cut_0_on_positive_strand: 233^234"
                     /note="cut_0_on_negative_strand: 237^238"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=NotI
ORIGIN
        1 gtcgacggta ccgccaccAT GACCATCGTC AACATCCACA GCACTCTGCT GTTCCTTTCG
       61 ATATTCTTCC CTCATAGACT CCGGCACTCC TTCCGTAGAA GGCCCTCTTT TCAAACCGTA
      121 TTTAAGGCGA CGATAAATGC ATTTGAAAAA AAGACGATCA AGAATCCACA ATATCAAGTG
      181 CAAGATCCCA ATGATACTTG CGGCAATAAC GAGAGGATCA CTTGActcga ggcggccgc
//