LOCUS       pOME0163R      1871 bp    DNA     circular UNA 17-Jan-2014
DEFINITION  CHIKV nsP4 Sri Lankan isolate
FEATURES             Location/Qualifiers
     Site            1..12
                     /site_type="mutagenized site"
                     /label="5' RE tag"
     Site            13..18
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             19..1857
                     /cds_type=ORF
                     /note="Length: 1839"
                     /note="Found at strand: positive"
                     /note="Start codon: atg"
     CDS             19..1857
                     /cds_type=ORF
                     /label="ORF -nsP4"
     Site            19..21
                     /site_type="mutagenized site"
                     /label=start
     Region          187..196
                     /note="Match: gcatccatgg"
                     /note="Length of match: 10"
                     /note="Accuracy (%): 80"
                     /note=Description:
                     /note="Found at strand: Positive"
                     /label=Kozak
     Region          187..196
                     /note="Match: gcatccatgg"
                     /note="Length of match: 10"
                     /note="Accuracy (%): 80"
                     /note=Description:
                     /note="Found at strand: Positive"
                     /label="Kozak 2"
     Region          1084..1093
                     /note="Match: gccatcatag"
                     /note="Length of match: 10"
                     /note="Accuracy (%): 80"
                     /note=Description:
                     /note="Found at strand: Positive"
                     /label=Kozak
     Region          1294..1303
                     /note="Match: ggcgccatga"
                     /note="Length of match: 10"
                     /note="Accuracy (%): 80"
                     /note=Description:
                     /note="Found at strand: Positive"
                     /label="Kozak 2"
     Region          1780..1789
                     /note="Match: gccacctttg"
                     /note="Length of match: 10"
                     /note="Accuracy (%): 80"
                     /note=Description:
                     /note="Found at strand: Positive"
                     /label=Kozak
     Site            1858..1871
                     /site_type="mutagenized site"
                     /label="3' RE tag"
ORIGIN
        1 gtcgacggta ccgccaccat gtatatattc tcgtcggaca ccggtccagg tcatttacaa
       61 cagaagtcag tacgccagtc agtgctgccg gtgaacaccc tggaggaagt ccacgaggag
      121 aagtgttacc cacctaagct ggatgaagca aaggagcaac tattacttaa gaaactccag
      181 gagagtgcat ccatggccaa cagaagcagg tatcagtcgc gcaaagtaga aaacatgaaa
      241 gcagcaatca tccagagact aaagagaggc tgtagactat acttaatgtc agagacccca
      301 aaagtcccta cttaccggac tacatatccg gcgcctgtgt actcgcctcc gatcaacgtt
      361 cgattgtcca atcccgagtc cgcagtggca gcatgcaatg agttcttagc tagaaactat
      421 ccaactgtct catcatacca aattaccgac gagtatgatg catatctaga catggtggac
      481 gggtcggaga gttgcctgga ccgagcgaca ttcaatccgt caaaactcag gagctacccg
      541 aaacagcacg cttaccacgc gccctccatc agaagcgctg taccgtcccc attccagaac
      601 acactacaga atgtactggc agcagccacg aaaagaaact gcaacgtcac acagatgagg
      661 gaattaccca ctttggactc agcagtattc aacgtggagt gtttcaaaaa attcgcatgc
      721 aaccaagaat actgggaaga atttgctgcc agccctatta ggataacaac tgagaattta
      781 gcaacctatg ttactaaact aaaagggcca aaagcagcag cgctattcgc aaaaacccat
      841 aatctactgc cactacagga agtaccaatg gataggttca cagtagatat gaaaagggac
      901 gtgaaggtga ctcctggtac aaagcataca gaggaaagac ctaaggtgca ggttatacag
      961 gcggctgaac ccttggcgac agcataccta tgtgggattc acagagagct ggttaggagg
     1021 ctgaacgccg tcctcctacc caatgtacat acactatttg acatgtctgc cgaggatttc
     1081 gatgccatca tagccgcaca ctttaagcca ggagacactg ttttggaaac ggacatagcc
     1141 tcctttgata agagccaaga tgattcactt gcgcttactg ctttgatgct gttagaggat
     1201 ttaggggtgg atcactccct gctggacttg atagaggctg ctttcggaga gatttccagc
     1261 tgtcacctac cgacaggtac gcgcttcaag ttcggcgcca tgatgaaatc aggtatgttc
     1321 ctaactctgt tcgtcaacac attgttaaac atcaccatcg ccagccgagt gctggaagat
     1381 cgtctgacaa aatccgcgtg cgcggccttc atcggcgacg acaacataat acatggagtc
     1441 gtctccgatg aattgatggc agccagatgt gccacttgga tgaacatgga agtgaagatc
     1501 atagatgcag ttgtatcctt gaaagcccct tacttttgtg gagggtttat actgcacgat
     1561 actgtgacag gaacagcttg cagagtggca gacccgctaa aaaggctttt taaactgggc
     1621 aaaccgctag cggcaggtga cgaacaagat gaagatagaa gacgagcgct ggctgacgaa
     1681 gtgatcagat ggcaacgaac agggctaatt gatgagctgg agaaagcggt atactctagg
     1741 tacgaagtgc agggtatatc agttgtggta atgtccatgg ccacctttgc aagctccaga
     1801 tccaatttcg agaagctcag aggacccgtc ataactttgt acggcggtcc taaatagctc
     1861 gaggcggccg c
//