LOCUS pOME0188R 350 bp DNA linear UNA 04-Mar-2014 DEFINITION MERS EMC JX8 E - TAGGED SOURCE UNC178-4 (MERS EMC JX8 E- TAGGED) FEATURES Location/Qualifiers Site 1..6 /site_type="restriction site" /label=SalI Site 1..12 /site_type="mutagenized site" /label="5' RE tag" Site 7..12 /site_type="restriction site" /label=KpnI Site 13..18 /site_type="mutagenized site" /label="Kozak sequence" CDS 19..333 /cds_type=ORF /note="Length: 315" /note="Found at strand: positive" /note="Start codon: ATG" Site 265..330 /site_type="mutagenized site" /label="3X FLAG tag" Site 331..336 /site_type="mutagenized site" /label="double stop" Site 337..342 /site_type="restriction site" /label=XhoI Site 337..350 /site_type="mutagenized site" /label="3' RE tag" Site 343..350 /site_type="restriction site" /label=NotI ORIGIN 1 gtcgacggta ccgccaccAT GTTACCCTTT GTCCAAGAAC GAATAGGGTT GTTCATAGTA 61 AACTTTTTCA TTTTTACCGT AGTATGTGCT ATAACACTCT TGGTGTGTAT GGCTTTCCTT 121 ACGGCTACTA GATTATGTGT GCAATGTATG ACAGGCTTCA ATACCCTGTT AGTTCAGCCC 181 GCATTATACT TGTATAATAC TGGACGTTCA GTCTATGTAA AATTCCAGGA TAGTAAACCC 241 CCTCTACCAC CTGACGAGTG GGTTgactac aaagaccatg acggtgatta taaagatcat 301 gatatcgatt acaaggatga cgatgacaag tagtaactcg aggcggccgc //