LOCUS       pOME0192       785 bp    DNA     linear   UNA 23-Jun-2014
DEFINITION  KSHV ORF55 tagged
FEATURES             Location/Qualifiers
     Site            1..6
                     /site_type="restriction site"
                     /label=SalI
     Site            1..12
                     /site_type="mutagenized site"
                     /label="5' RE tag"
     Site            7..12
                     /site_type="restriction site"
                     /label=KpnI
     Site            13..18
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             19..768
                     /cds_type=ORF
                     /note="Length: 750"
                     /note="Found at strand: positive"
                     /note="Start codon: Atg"
     Site            696..765
                     /site_type="mutagenized site"
                     /label="3X FLAG tag"
     Site            766..771
                     /site_type="mutagenized site"
                     /label="double stop"
     Site            772..777
                     /site_type="restriction site"
                     /label=XhoI
     Site            772..785
                     /site_type="mutagenized site"
                     /label="3' RE tag"
     Site            778..785
                     /site_type="restriction site"
                     /label=NotI
ORIGIN
        1 gtcgacggta ccgccaccAt gtcgtctcca tggtacacct ggacgtgttg cggtataaac
       61 ctttttggac gcggcaacca cgcttacaag cgcctcggtg accctctgga aggctgtcca
      121 gagcgctggc ggcaagagat agatttgggc ctcccccccg gagtgtgtct gggtgacgtt
      181 gtccagtcga accttggcac gacggcgctg caccagacct accttctggc tgtacagtca
      241 aacaagatca cagactacct gaagcgtttt gacgtggcta aaattccggc gggctgccag
      301 gagaccgtga aaacccaggt aaaaaaactg cagtctattc agaacgtcgt gtggaacaca
      361 atgctggcct tggcggtcgg cgagattacc gtggacgaca gcgcgctcca gtccctctta
      421 aacaaaaggg ccggggaatg cgtttcgctc atggagatgg aaaaactggc gactgctatg
      481 gcgtcagacg actcggtaat atgggcctct gagatcagcc actccttgtc ggaacccact
      541 tctgtattgc ctctcacgcc tgccgttacg cgccagccgg aggcaacttt acccaagcca
      601 cccaccgaag acccgtccgt ttccgcgatg cattcgtcaa tccctccccg gccttcgtca
      661 accctggaag agacgacaga aagcgcgata ggttcgacag actacaaaga ccatgacggt
      721 gattataaag atcatgatat cgattacaag gatgacgatg acaagtagta actcgaggcg
      781 gccgc
//