LOCUS pOME0192 785 bp DNA linear UNA 23-Jun-2014 DEFINITION KSHV ORF55 tagged FEATURES Location/Qualifiers Site 1..6 /site_type="restriction site" /label=SalI Site 1..12 /site_type="mutagenized site" /label="5' RE tag" Site 7..12 /site_type="restriction site" /label=KpnI Site 13..18 /site_type="mutagenized site" /label="Kozak sequence" CDS 19..768 /cds_type=ORF /note="Length: 750" /note="Found at strand: positive" /note="Start codon: Atg" Site 696..765 /site_type="mutagenized site" /label="3X FLAG tag" Site 766..771 /site_type="mutagenized site" /label="double stop" Site 772..777 /site_type="restriction site" /label=XhoI Site 772..785 /site_type="mutagenized site" /label="3' RE tag" Site 778..785 /site_type="restriction site" /label=NotI ORIGIN 1 gtcgacggta ccgccaccAt gtcgtctcca tggtacacct ggacgtgttg cggtataaac 61 ctttttggac gcggcaacca cgcttacaag cgcctcggtg accctctgga aggctgtcca 121 gagcgctggc ggcaagagat agatttgggc ctcccccccg gagtgtgtct gggtgacgtt 181 gtccagtcga accttggcac gacggcgctg caccagacct accttctggc tgtacagtca 241 aacaagatca cagactacct gaagcgtttt gacgtggcta aaattccggc gggctgccag 301 gagaccgtga aaacccaggt aaaaaaactg cagtctattc agaacgtcgt gtggaacaca 361 atgctggcct tggcggtcgg cgagattacc gtggacgaca gcgcgctcca gtccctctta 421 aacaaaaggg ccggggaatg cgtttcgctc atggagatgg aaaaactggc gactgctatg 481 gcgtcagacg actcggtaat atgggcctct gagatcagcc actccttgtc ggaacccact 541 tctgtattgc ctctcacgcc tgccgttacg cgccagccgg aggcaacttt acccaagcca 601 cccaccgaag acccgtccgt ttccgcgatg cattcgtcaa tccctccccg gccttcgtca 661 accctggaag agacgacaga aagcgcgata ggttcgacag actacaaaga ccatgacggt 721 gattataaag atcatgatat cgattacaag gatgacgatg acaagtagta actcgaggcg 781 gccgc //