LOCUS       pOME0236P      1273 bp    DNA     linear   VRL 27-AUG-2012
DEFINITION  anti-PAN Human herpesvirus 8 strain JSC-1 clone BAC16, complete genome.
ACCESSION   GQ994935
VERSION     GQ994935.1  GI:261853473
KEYWORDS    .
SOURCE      Human herpesvirus 8 (Kaposi's sarcoma-associated herpesvirus)
  ORGANISM  Human herpesvirus 8
            Viruses; dsDNA viruses, no RNA stage; Herpesvirales; Herpesviridae;
            Gammaherpesvirinae; Rhadinovirus.
REFERENCE   1  (bases 1 to 138146)
  AUTHORS   Brulois,K.F., Chang,H., Lee,A.S., Ensser,A., Wong,L.Y., Toth,Z.,
            Lee,S.H., Lee,H.R., Myoung,J., Ganem,D., Oh,T.K., Kim,J.F.,
            Gao,S.J. and Jung,J.U.
  TITLE     Construction and manipulation of a new Kaposi's sarcoma-associated
            herpesvirus Bacterial artificial chromosome clone
  JOURNAL   J. Virol. 86 (18), 9708-9720 (2012)
   PUBMED   22740391
REFERENCE   2  (bases 1 to 138146)
  AUTHORS   Brulois,K., Lee,A. and Chang,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2009) Molecular Microbiology and Immunology,
            University of Southern California, 1450 Biggy Street, Los Angeles,
            CA 90033, USA
FEATURES             Location/Qualifiers
     source          complement(<1..>1273)
                     /organism="Human herpesvirus 8"
                     /mol_type="genomic DNA"
                     /strain="JSC-1"
                     /db_xref="taxon:37296"
                     /clone="BAC16"
                     /note="infectious BAC clone harboring full-length KSHV
                     genome; derived from rKSHV.219, KSHV-infected JSC1
                     primary effusion lymphoma cell line"
     Site            1..6
                     /site_type="mutagenized site"
                     /label="5' RE site"
     polyA_signal    complement(<7..12)
                     /note="corresp. pos."
                     /label="PAS K4.5-PAN-K7 - Gao lab 2014"
     misc_RNA        complement(<7..1063)
                     /note="corresp. pos."
                     /label="PAN RNA - NC_009333 genome annotation"
     misc_RNA        complement(<7..1064)
                     /note="from 2014 Arias et al. (Ganem lab) KSHV 2.0"
                     /label="PAN RNA - Ganem lab 2014"
     misc_RNA        complement(<7..1069)
                     /note="corresp. pos."
                     /label="PAN RNA - Zheng lab 2013"
     misc_RNA        complement(<7..>1267)
                     /note="5' end not determine, est. based on Fig. 2"
                     /label="K4.5 (T6.1, as-K5-K6) - Ganem lab 2014"
     misc_RNA        7..1267
                     /note="5' end approx."
                     /note="corresp. pos."
                     /label="K7.3 (anti-PAN lncRNA) - Chang/Moore lab 2011"
     misc_RNA        complement(<7..>1267)
                     /note="corresp. to 5' end ID'd by 2005 Taylor et al.
                     (Chang/Moore lab)"
                     /note="corresp. to PAN RNA 3' end ID'd by KSHV 2.0"
                     /note="corresp. pos."
                     /label="K4.5 (T6.1) - Chang/Moore lab 2005/2011, Ganem
                     lab 2014"
     polyA_site      complement(453)
                     /note="corresp. pos."
                     /label="TTS K4.5(T6.1)-PAN-K7 - Zheng lab 2013"
     CDS             complement(728..1108)
                     /codon_start=1
                     /product="K7"
                     /protein_id="ACY00412.1"
                     /db_xref="GI:261853490"
                     /translation="MGTLEIKGASLSQFSTGTAQSPWLPLHLWILCSLLAFLPLLVFI
                     GAADCGLIASLLAIYPSWLSARFSVLLFPHWPESCSTKNTARSGALHKPAEQKLRFAQ
                     KPCHGNYTVTPCGLLHWIQSPGQL"
     polyA_site      complement(805)
                     /note="corresp. pos."
                     /label="TTS K4.5(T6.1)-PAN - Zheng lab 2013"
     polyA_signal    1263..>1267
                     /label="PAS K7.3(anti-PAN lncRNA) - Schifano guess"
     Site            1268..1273
                     /site_type="mutagenized site"
                     /label="3' RE site"
ORIGIN
        1 ggatccTTAT TTATGTTGTA AGTTGCATTA CGTTATGGTA TTAGTTTAAT TTGAGCTCTA
       61 GGCACGTTAA ATTGTCAAAA GTATAACATG TTTTTCCAAT ATACACTGGG ATAAAAACCT
      121 TGCCGTCTGG TCACTGGAGG TGTCCGGTGC GAACAAGGAA AAACCCAGCC AAAACACCGT
      181 TATCATTGTT ACACAACGCT TTCACCTACA AGAAAACATC GTTAGTCAAC CTAGCAAAAC
      241 ATCAAATATT AGCCTGGAAC TTCCAACACA ACATACACAT TAAAACATTA ACATTGAAAG
      301 AGCGCTCCCA GCTGCCGCAC ACCACTTTAG TCCAATGTTC TTACACGACT TTGAAACTTC
      361 TGACAAATGC CACCTCACTT TGTCGCCTAT GTCATTCAAA TCGACTTGCT TACACTGGAA
      421 AAATAAACAC ACCATTACAG CACTAGCCTG ATACAATCTA AAACGCATTT TAAAATGCTT
      481 CACAACGCAC CAATAAGATA CACATCCAGA TTGTCACATT TAGGGCAAAG TGGCCCGATT
      541 TACACTCAAT CCGCTTTCTA GAATTACCTC AACACTATCT AAGAATCAGA CAAACACAGA
      601 ACCGAAACAA CGAATGAGCA GATAGGTAGT GCACCACTGT TCTGATACAC CAGTGGGCGC
      661 TGCTTTCCTT TCACATTATA TTCCACATTC AGACACGTTA AGTATCCTCG CATATCATAA
      721 AAGGGGGCTA CAACTGGCCT GGAGATTGAA TCCAATGCAA TAACCCGCAA GGGGTGACTG
      781 TATAGTTGCC ATGGCAAGGT TTTTGGGCAA ATCGCAGCTT TTGTTCTGCG GGCTTATGGA
      841 GAGCTCCAGA CCGCGCGGTG TTTTTTGTAC TACAGCTCTC AGGCCAATGT GGGAAAAGTA
      901 GGACGGAAAA CCTAGCCGAA AGCCAGGATG GGTATATTGC CAAAAGCGAC GCAATCAACC
      961 CACAATCGGC GGCACCAATG AAAACCAGAA GCGGCAAGAA GGCAAGCAGC GAGCACAAAA
     1021 TCCATAGGTG AAGCGGCAGC CAAGGTGACT GGGCAGTCCC AGTGCTAAAC TGACTCAAGC
     1081 TGGCCCCTTT TATCTCCAGT GTTCCCATAT TTTGGACAGG TTAGCCACCC ATTTTTGGAA
     1141 GCCACGCCCC TTTATGTCAT TAACATCATC CTTCAAGCTG ACCCTTAATA ATCCATAAGA
     1201 AAACTCCATA CTCCTACCAA ATATGGAAAC CAAATTACAT ATACACAATA CGGATGAACA
     1261 TTAATAAgaa ttc
//