LOCUS pOME0238P 2925 bp DNA linear VRL 27-AUG-2012 DEFINITION antiRTA3kb Human herpesvirus 8 strain JSC-1 clone BAC16, complete genome. ACCESSION GQ994935 VERSION GQ994935.1 GI:261853473 KEYWORDS . SOURCE Human herpesvirus 8 (Kaposi's sarcoma-associated herpesvirus) ORGANISM Human herpesvirus 8 Viruses; dsDNA viruses, no RNA stage; Herpesvirales; Herpesviridae; Gammaherpesvirinae; Rhadinovirus. REFERENCE 1 (bases 1 to 138146) AUTHORS Brulois,K.F., Chang,H., Lee,A.S., Ensser,A., Wong,L.Y., Toth,Z., Lee,S.H., Lee,H.R., Myoung,J., Ganem,D., Oh,T.K., Kim,J.F., Gao,S.J. and Jung,J.U. TITLE Construction and manipulation of a new Kaposi's sarcoma-associated herpesvirus Bacterial artificial chromosome clone JOURNAL J. Virol. 86 (18), 9708-9720 (2012) PUBMED 22740391 REFERENCE 2 (bases 1 to 138146) AUTHORS Brulois,K., Lee,A. and Chang,H. TITLE Direct Submission JOURNAL Submitted (21-SEP-2009) Molecular Microbiology and Immunology, University of Southern California, 1450 Biggy Street, Los Angeles, CA 90033, USA FEATURES Location/Qualifiers source complement(<1..>2925) /organism="Human herpesvirus 8" /mol_type="genomic DNA" /strain="JSC-1" /db_xref="taxon:37296" /clone="BAC16" /note="infectious BAC clone harboring full-length KSHV genome; derived from rKSHV.219, KSHV-infected JSC1 primary effusion lymphoma cell line" misc_RNA 7..1038 /note="corresp. pos." /label="T1.2 lncRNA - Yuan lab 2002" misc_RNA 7..1138 /note="corresp. pos." /label="T1.2 lncRNA - Ganem lab 2010 (Xu et al.)" misc_RNA 7..2917 /note="corresp. pos." /label="K7.7 (T3.0 lncRNA) - Yuan lab 2002" misc_RNA 7..2917 /note="corresp. pos." /label="K7.7 (T3.0 lncRNA) - Ganem lab 2010 (Xu et al.)" CDS complement(7..2917) /cds_type=ORF /label="3kb antiRTA" polyA_signal 1002..1007 /label="PAS T1.2 lncRNA - Schifano guess" polyA_site 1039 /note="corresp. pos." /label="TTS K7.7 (T3.0 lncRNA) - Zheng lab 2013" CDS 1986..2894 /codon_start=1 /product="ORF49" /protein_id="ACY00447.1" /db_xref="GI:261853525" /translation="MTSRRPLKDHLFNHLFRYHYPSWDQILQELDTLSVATLNPDCHV PALNVEKTLYLAKTIQILVQHRQSEPYLVPAARANLAYSLQQLYKLGNDKIRGVINGM LPLVDAGCIGFERELIKGLPRVLTLQYPHTAPLESEPPTADCTEWCLSHFVGASGRLR SEVRDILTTHNGTCAPSFQWMASVVKKFFLVETVIYEDFQDTDFNVQLNLCFFWTAVV QMYQRCIYEQKLVHIISTSLTLLKSTARSFFAWYDLYRPNLGSAALVKYTEHLIRALT PDCSDVELGELCSHLHHCKHALFSIQ" polyA_signal 2890..2895 /note="corresp. pos." /label="PAS ORF49 - Gao lab 2014" polyA_site 2906 /note="corresp. pos." /label="TTS ORF49 - Damania lab 2006" polyA_site 2909 /note="corresp. pos." /label="TTS ORF49 - Gao lab 2014" Site 2918..2925 /site_type="mutagenized site" /label="3' RE site" ORIGIN 1 gaattcTCTC CACCAGAAGG TGACGGTATA TCCGCCGGAG ACTCCGGGCC GAAGGAAGCG 61 GGGGAGGGTG ACCGCAGAGG CGGTGGCGTG TCCAGCTGGT ACAGTCCTTG CAGAATACTG 121 GACAACAGCG CGTCGTCAGT GGGGGAGAGA GGCGACCCGA CATCCCATGT TTCAGGGCCC 181 GCTTCGTCTA ACAGGGCGAA TAGACTCTGA TCTACGTCCA GTGGCGGGGG TAACGACAGT 241 AGCGGGGGTT GTGCCTCCGA TGGTCCCTCA GACGTGGCGA CATCCGTGGC GTGCACATCC 301 TGGGGGGTGG TAATTGATCC CAAAGAGGTA CCAGGTGTCG TGGTCGATGG CGTGGTGTCG 361 GCTTCTGGCA CGGTCAAAGC CTTACGCTTC TTTGAGCTCC TCTCTTTGCT TCTCTGCTTT 421 CGTTTGGGTG TTGTCGCCTC TGCCGCCGTA GCCGCCGCCG TTGAATCTCT ACACGGCACA 481 CCTTGGTCTC CGTCATGACC GCCCGGAAGC TCTTCGCCCG AGGCTTCTGG TTGGGCGAGC 541 GCCGGGAGGA ACGCGCTGGC CGGCAGAGGG ACTACTGTTG GCAACTGCAC CACCGGGGCC 601 CGGACTGTTG AATCGGGGGG TGGTGTCGAA GAGGGGGCAC AGGTAACCTG CAACAACGTA 661 ACGCATTGCG GTGGTTGAAA TTGCTGGGCC ACTATAACCG TGTTCGGATC GCAAGGGTGA 721 CATGACGTCA GGAAAGAGCT CGGGTTGTCG GGAGAATCTC CGGTGTTCTC TGCGACAAAA 781 CATGCAGCGA CCTGATCTCT TGCAGGAGTG GACGCTGACA TTACAGGCAA CTCCCCTCCG 841 GTACCCCGCG CTGGCATTGC CTGCGGCACC GTTGTCGCAG TAATCACGGC CCCTTGAGCA 901 ACCGCCTTGA CTGACACCGT CTGTGAACTA CCGGTGGCCG GTTTTAAGGG CGCGGTGGAT 961 GTGTTGTACA CCATATTCTG TGACGCGGCC TGCCACGTAC TAATAAACGC CGGGTCTGTA 1021 CCCGATGGGT TAAAGGGGAT GATGCTGACG GTGTGCGGCA TGTTGGGATG GGGTTTGCTA 1081 ATGACAAACT GGCTCAGGGC AGGGGCGCTC AGAATAACGG CGGGTTTTGC CAGCACATCG 1141 CTGAGGTCTA CCATGGAAGC CGGCAACAGT CCTGGCTCCA GGATTGGCAA AATTAAAGAT 1201 TCTGGCATGA GACCGCTTCC TGGATAGGAC AGTCCAGTGA CCTTGCCGGT GGTAATTGGC 1261 CGGCGTTTCT CAGCAGAACC GGTGATGCTG TAAAAGTTTT CAGGGGCGAG CTGACCCTTG 1321 GTCATGATCT CCAGCGGCGC CATAATCCTC GGAAGGTAGA CCGGTTGGAA AACCAAAAGG 1381 CAGAGGGCCC TGGACTGCTC AGAGAGCAGC CCGGCGGTAT CGTACGTGTT GTAGAGCTTC 1441 AGTAGGGAAG AGGTTAATAT CATAATGGGC CTCCGATTGC AGACGAGTCG CCTCCAGGCC 1501 CCCAGGCATT TGGCCTTCAT TTCAGTGTAT ATTTCGTGTA GTAGCTTACA TATGGCTGGA 1561 GGCAGGCTCA CCCCGCTGCA TGCGGCTGTC AGAAATGCCT TTTCTGTGCA GAATAAGATC 1621 CTCTCCGTGA GGATCCGAAT AATGCCTTGG GATGCCTCTG CGCATGGCAC GTTGTACCCG 1681 GCCGCGCCTT TGGTCCTCAT TCGAGAGGCA CGAAAAAAGC AGAAAAGGTT AAACATGTTA 1741 ATGTCTGTAA ACAGACCACA TAGCGCACCA AGCTTGGAAC ATTCTTTCAT AATCCGAATG 1801 CACACATCTT CCACCACTCT ATTTAGGTCA CTGGGATCGT AGATTGTTTC GTATGCGTCA 1861 TTTATAAGGA GCACACACTG GTAGAGTTGG GCCTTCAGTT CGTCCGAGAG GCCGACGAAG 1921 CTTTCCACAC AGGACCGCCG AAGCTTCTTA CCCTAAGGAG AGAGTGGCGT GTCATAGTTT 1981 TTACAATGAC ATCGAGAAGG CCCCTTAAAG ATCACCTGTT CAACCATCTG TTTAGGTATC 2041 ATTACCCGTC CTGGGATCAG ATACTTCAGG AGTTAGATAC CCTGTCCGTC GCCACCCTCA 2101 ATCCAGACTG CCACGTACCC GCTCTAAACG TTGAGAAGAC CCTATACCTT GCAAAGACCA 2161 TTCAGATATT GGTCCAGCAC CGACAAAGCG AGCCGTACCT GGTTCCCGCG GCTAGGGCGA 2221 ATTTGGCTTA TTCCCTGCAG CAGTTGTACA AACTTGGCAA CGACAAGATA AGGGGTGTTA 2281 TTAACGGCAT GCTGCCACTA GTGGACGCTG GCTGCATAGG TTTTGAGAGA GAGCTCATTA 2341 AGGGACTGCC CAGAGTTTTG ACTCTCCAAT ACCCCCACAC GGCGCCCCTG GAATCAGAAC 2401 CCCCGACCGC GGACTGCACA GAGTGGTGCC TCTCCCATTT TGTGGGAGCG TCCGGCCGTC 2461 TCCGCTCCGA AGTTAGGGAT ATCCTAACAA CGCACAACGG GACGTGTGCT CCCTCCTTTC 2521 AGTGGATGGC CAGCGTGGTA AAAAAGTTTT TCTTGGTAGA AACTGTCATT TACGAGGACT 2581 TTCAGGATAC AGATTTTAAC GTGCAGCTGA ACCTTTGTTT TTTTTGGACA GCTGTCGTTC 2641 AGATGTACCA GCGGTGCATT TACGAGCAGA AGCTTGTCCA CATAATCAGC ACGTCCCTAA 2701 CTCTCCTGAA AAGCACCGCC CGATCCTTTT TTGCCTGGTA CGACCTGTAC AGACCCAACC 2761 TAGGTTCCGC GGCTTTGGTC AAGTACACCG AACACTTAAT AAGAGCCCTG ACACCCGACT 2821 GTTCGGACGT AGAGCTTGGC GAACTCTGCT CCCACCTACA CCATTGTAAA CACGCATTGT 2881 TCAGTATACA ATAAAAAGGT CGATCTTTAC CTTGTCAgcg gccgc //