LOCUS       pOME0242P      1045 bp    DNA     linear   VRL 27-AUG-2012
DEFINITION  antiRTA1kb Human herpesvirus 8 strain JSC-1 clone BAC16, complete genome.
ACCESSION   GQ994935
VERSION     GQ994935.1  GI:261853473
KEYWORDS    .
SOURCE      Human herpesvirus 8 (Kaposi's sarcoma-associated herpesvirus)
  ORGANISM  Human herpesvirus 8
            Viruses; dsDNA viruses, no RNA stage; Herpesvirales; Herpesviridae;
            Gammaherpesvirinae; Rhadinovirus.
REFERENCE   1  (bases 1 to 138146)
  AUTHORS   Brulois,K.F., Chang,H., Lee,A.S., Ensser,A., Wong,L.Y., Toth,Z.,
            Lee,S.H., Lee,H.R., Myoung,J., Ganem,D., Oh,T.K., Kim,J.F.,
            Gao,S.J. and Jung,J.U.
  TITLE     Construction and manipulation of a new Kaposi's sarcoma-associated
            herpesvirus Bacterial artificial chromosome clone
  JOURNAL   J. Virol. 86 (18), 9708-9720 (2012)
   PUBMED   22740391
REFERENCE   2  (bases 1 to 138146)
  AUTHORS   Brulois,K., Lee,A. and Chang,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2009) Molecular Microbiology and Immunology,
            University of Southern California, 1450 Biggy Street, Los Angeles,
            CA 90033, USA
FEATURES             Location/Qualifiers
     misc_RNA        <1..>1045
                     /note="corresp. pos."
                     /label="K7.7 (T3.0 lncRNA) - Ganem lab 2010 (Xu et al.)"
     misc_RNA        1..>1045
                     /note="corresp. pos."
                     /label="K7.7 (T3.0 lncRNA) - Yuan lab 2002"
     misc_RNA        1..1045
                     /note="corresp. pos."
                     /label="T1.2 lncRNA - Yuan lab 2002"
     misc_RNA        <1..>1045
                     /note="corresp. pos."
                     /label="T1.2 lncRNA - Ganem lab 2010 (Xu et al.)"
     Site            1..6
                     /site_type="restriction site"
                     /label=BamHI
     source          complement(<1..>1045)
                     /organism="Human herpesvirus 8"
                     /mol_type="genomic DNA"
                     /strain="JSC-1"
                     /db_xref="taxon:37296"
                     /clone="BAC16"
                     /note="infectious BAC clone harboring full-length KSHV
                     genome; derived from rKSHV.219, KSHV-infected JSC1
                     primary effusion lymphoma cell line"
     CDS             complement(<7..>1039)
                     /cds_type=ORF
                     /codon_start=1
                     /product="ORF50"
                     /protein_id="ACY00448.1"
                     /db_xref="GI:261853526"
                     /translation="MAQDDKGKKLRRSCVESFVGLSDELKAQLYQCVLLINDAYETIY
                     DPSDLNRVVEDVCIRIMKECSKLGALCGLFTDINMFNLFCFFRASRMRTKGAAGYNVP
                     CAEASQGIIRILTERILFCTEKAFLTAACSGVSLPPAICKLLHEIYTEMKAKCLGAWR
                     RLVCNRRPIMILTSSLLKLYNTYDTAGLLSEQSRALCLLVFQPVYLPRIMAPLEIMTK
                     GQLAPENFYSITGSAEKRRPITTGKVTGLSYPGSGLMPESLILPILEPGLLPASMVDL
                     SDVLAKPAVILSAPALSQFVISKPHPNMPHTVSIIPFNPSGTDPAFISTWQAASQNMV
                     YNTSTAPLKPATGSSQTVSVKAVAQGAVITATTVPQAMPARGTGGELPVMSASTPARD
                     QVAACFVAENTGDSPDNPSSFLTSCHPCDPNTVIVAQQFQPPQCVTLLQVTCAPSSTP
                     PPDSTVRAPVVQLPTVVPLPASAFLPALAQPEASGEELPGGHDGDQGVPCRDSTAAAT
                     AAEATTPKRKQRSKERSSKKRKALTVPEADTTPSTTTPGTSLGSITTPQDVHATDVAT
                     SEGPSEAQPPLLSLPPPLDVDQSLFALLDEAGPETWDVGSPLSPTDDALLSSILQGLY
                     QLDTPPPLRSPSPASFGPESPADIPSPSGGEYTQLQPVRATSATPANEVQESGTLYQL
                     HQWRNYFRD"
                     /label="ORF50 (RTA)"
                     /label="AntiRTA 1kb"
     polyA_signal    1003..1008
                     /label="PAS T1.2 lncRNA - Schifano guess"
     Site            1040..1045
                     /site_type="restriction site"
                     /label=EcoRI
ORIGIN
        1 ggatccCTCT CCACCAGAAG GTGACGGTAT ATCCGCCGGA GACTCCGGGC CGAAGGAAGC
       61 GGGGGAGGGT GACCGCAGAG GCGGTGGCGT GTCCAGCTGG TACAGTCCTT GCAGAATACT
      121 GGACAACAGC GCGTCGTCAG TGGGGGAGAG AGGCGACCCG ACATCCCATG TTTCAGGGCC
      181 CGCTTCGTCT AACAGGGCGA ATAGACTCTG ATCTACGTCC AGTGGCGGGG GTAACGACAG
      241 TAGCGGGGGT TGTGCCTCCG ATGGTCCCTC AGACGTGGCG ACATCCGTGG CGTGCACATC
      301 CTGGGGGGTG GTAATTGATC CCAAAGAGGT ACCAGGTGTC GTGGTCGATG GCGTGGTGTC
      361 GGCTTCTGGC ACGGTCAAAG CCTTACGCTT CTTTGAGCTC CTCTCTTTGC TTCTCTGCTT
      421 TCGTTTGGGT GTTGTCGCCT CTGCCGCCGT AGCCGCCGCC GTTGAATCTC TACACGGCAC
      481 ACCTTGGTCT CCGTCATGAC CGCCCGGAAG CTCTTCGCCC GAGGCTTCTG GTTGGGCGAG
      541 CGCCGGGAGG AACGCGCTGG CCGGCAGAGG GACTACTGTT GGCAACTGCA CCACCGGGGC
      601 CCGGACTGTT GAATCGGGGG GTGGTGTCGA AGAGGGGGCA CAGGTAACCT GCAACAACGT
      661 AACGCATTGC GGTGGTTGAA ATTGCTGGGC CACTATAACC GTGTTCGGAT CGCAAGGGTG
      721 ACATGACGTC AGGAAAGAGC TCGGGTTGTC GGGAGAATCT CCGGTGTTCT CTGCGACAAA
      781 ACATGCAGCG ACCTGATCTC TTGCAGGAGT GGACGCTGAC ATTACAGGCA ACTCCCCTCC
      841 GGTACCCCGC GCTGGCATTG CCTGCGGCAC CGTTGTCGCA GTAATCACGG CCCCTTGAGC
      901 AACCGCCTTG ACTGACACCG TCTGTGAACT ACCGGTGGCC GGTTTTAAGG GCGCGGTGGA
      961 TGTGTTGTAC ACCATATTCT GTGACGCGGC CTGCCACGTA CTAATAAACG CCGGGTCTGT
     1021 ACCCGATGGG TTAAAGGGGg aattc
//