LOCUS       pOME0243M      1050 bp    DNA     linear   VRL 27-AUG-2012
DEFINITION  Reverse compl antiRTA1kb pOME0242 Human herpesvirus 8 strain JSC-1 clone BAC16, complete genome.
ACCESSION   GQ994935
VERSION     GQ994935.1  GI:261853473
KEYWORDS    .
SOURCE      Human herpesvirus 8 (Kaposi's sarcoma-associated herpesvirus)
  ORGANISM  Human herpesvirus 8
            Viruses; dsDNA viruses, no RNA stage; Herpesvirales; Herpesviridae;
            Gammaherpesvirinae; Rhadinovirus.
REFERENCE   1  (bases 1 to 138146)
  AUTHORS   Brulois,K.F., Chang,H., Lee,A.S., Ensser,A., Wong,L.Y., Toth,Z.,
            Lee,S.H., Lee,H.R., Myoung,J., Ganem,D., Oh,T.K., Kim,J.F.,
            Gao,S.J. and Jung,J.U.
  TITLE     Construction and manipulation of a new Kaposi's sarcoma-associated
            herpesvirus Bacterial artificial chromosome clone
  JOURNAL   J. Virol. 86 (18), 9708-9720 (2012)
   PUBMED   22740391
REFERENCE   2  (bases 1 to 138146)
  AUTHORS   Brulois,K., Lee,A. and Chang,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2009) Molecular Microbiology and Immunology,
            University of Southern California, 1450 Biggy Street, Los Angeles,
            CA 90033, USA
COMMENT     
FEATURES             Location/Qualifiers
     misc_RNA        <1..>1050
                     /note="corresp. pos."
                     /label="K7.7 (T3.0 lncRNA) - Ganem lab 2010 (Xu et al.)"
     misc_RNA        <1..1050
                     /note="corresp. pos."
                     /label="K7.7 (T3.0 lncRNA) - Yuan lab 2002"
     misc_RNA        1..1050
                     /note="corresp. pos."
                     /label="T1.2 lncRNA - Yuan lab 2002"
     misc_RNA        <1..>1050
                     /note="corresp. pos."
                     /label="T1.2 lncRNA - Ganem lab 2010 (Xu et al.)"
     Site            1..6
                     /site_type="restriction site"
                     /label=EcoRI
     source          complement(<1..>1050)
                     /organism="Human herpesvirus 8"
                     /mol_type="genomic DNA"
                     /strain="JSC-1"
                     /db_xref="taxon:37296"
                     /clone="BAC16"
                     /note="infectious BAC clone harboring full-length KSHV
                     genome; derived from rKSHV.219, KSHV-infected JSC1
                     primary effusion lymphoma cell line"
     Site            1..1050
                     /site_type="mutagenized site"
                     /label="3' RE site"
     CDS             complement(<7..>1039)
                     /cds_type=ORF
                     /codon_start=1
                     /product="ORF50"
                     /protein_id="ACY00448.1"
                     /db_xref="GI:261853526"
                     /translation="MAQDDKGKKLRRSCVESFVGLSDELKAQLYQCVLLINDAYETIY
                     DPSDLNRVVEDVCIRIMKECSKLGALCGLFTDINMFNLFCFFRASRMRTKGAAGYNVP
                     CAEASQGIIRILTERILFCTEKAFLTAACSGVSLPPAICKLLHEIYTEMKAKCLGAWR
                     RLVCNRRPIMILTSSLLKLYNTYDTAGLLSEQSRALCLLVFQPVYLPRIMAPLEIMTK
                     GQLAPENFYSITGSAEKRRPITTGKVTGLSYPGSGLMPESLILPILEPGLLPASMVDL
                     SDVLAKPAVILSAPALSQFVISKPHPNMPHTVSIIPFNPSGTDPAFISTWQAASQNMV
                     YNTSTAPLKPATGSSQTVSVKAVAQGAVITATTVPQAMPARGTGGELPVMSASTPARD
                     QVAACFVAENTGDSPDNPSSFLTSCHPCDPNTVIVAQQFQPPQCVTLLQVTCAPSSTP
                     PPDSTVRAPVVQLPTVVPLPASAFLPALAQPEASGEELPGGHDGDQGVPCRDSTAAAT
                     AAEATTPKRKQRSKERSSKKRKALTVPEADTTPSTTTPGTSLGSITTPQDVHATDVAT
                     SEGPSEAQPPLLSLPPPLDVDQSLFALLDEAGPETWDVGSPLSPTDDALLSSILQGLY
                     QLDTPPPLRSPSPASFGPESPADIPSPSGGEYTQLQPVRATSATPANEVQESGTLYQL
                     HQWRNYFRD"
                     /label="ORF50 (RTA)"
                     /label="reverse compliment AntiRTA 1kb"
     Site            1040..1045
                     /site_type="restriction site"
                     /label=BamHI
ORIGIN
        1 gaattcCCCC TTTAACCCAT CGGGTACAGA CCCGGCGTTT ATTAGTACGT GGCAGGCCGC
       61 GTCACAGAAT ATGGTGTACA ACACATCCAC CGCGCCCTTA AAACCGGCCA CCGGTAGTTC
      121 ACAGACGGTG TCAGTCAAGG CGGTTGCTCA AGGGGCCGTG ATTACTGCGA CAACGGTGCC
      181 GCAGGCAATG CCAGCGCGGG GTACCGGAGG GGAGTTGCCT GTAATGTCAG CGTCCACTCC
      241 TGCAAGAGAT CAGGTCGCTG CATGTTTTGT CGCAGAGAAC ACCGGAGATT CTCCCGACAA
      301 CCCGAGCTCT TTCCTGACGT CATGTCACCC TTGCGATCCG AACACGGTTA TAGTGGCCCA
      361 GCAATTTCAA CCACCGCAAT GCGTTACGTT GTTGCAGGTT ACCTGTGCCC CCTCTTCGAC
      421 ACCACCCCCC GATTCAACAG TCCGGGCCCC GGTGGTGCAG TTGCCAACAG TAGTCCCTCT
      481 GCCGGCCAGC GCGTTCCTCC CGGCGCTCGC CCAACCAGAA GCCTCGGGCG AAGAGCTTCC
      541 GGGCGGTCAT GACGGAGACC AAGGTGTGCC GTGTAGAGAT TCAACGGCGG CGGCTACGGC
      601 GGCAGAGGCG ACAACACCCA AACGAAAGCA GAGAAGCAAA GAGAGGAGCT CAAAGAAGCG
      661 TAAGGCTTTG ACCGTGCCAG AAGCCGACAC CACGCCATCG ACCACGACAC CTGGTACCTC
      721 TTTGGGATCA ATTACCACCC CCCAGGATGT GCACGCCACG GATGTCGCCA CGTCTGAGGG
      781 ACCATCGGAG GCACAACCCC CGCTACTGTC GTTACCCCCG CCACTGGACG TAGATCAGAG
      841 TCTATTCGCC CTGTTAGACG AAGCGGGCCC TGAAACATGG GATGTCGGGT CGCCTCTCTC
      901 CCCCACTGAC GACGCGCTGT TGTCCAGTAT TCTGCAAGGA CTGTACCAGC TGGACACGCC
      961 ACCGCCTCTG CGGTCACCCT CCCCCGCTTC CTTCGGCCCG GAGTCTCCGG CGGATATACC
     1021 GTCACCTTCT GGTGGAGAGg gatccgatcc
//