LOCUS       pOME0244P      1093 bp    DNA     linear   VRL 27-AUG-2012
DEFINITION  PAN-RNA Human herpesvirus 8 strain JSC-1 clone BAC16, complete genome.
ACCESSION   GQ994935
VERSION     GQ994935.1  GI:261853473
KEYWORDS    .
SOURCE      Human herpesvirus 8 (Kaposi's sarcoma-associated herpesvirus)
  ORGANISM  Human herpesvirus 8
            Viruses; dsDNA viruses, no RNA stage; Herpesvirales; Herpesviridae;
            Gammaherpesvirinae; Rhadinovirus.
REFERENCE   1  (bases 1 to 138146)
  AUTHORS   Brulois,K.F., Chang,H., Lee,A.S., Ensser,A., Wong,L.Y., Toth,Z.,
            Lee,S.H., Lee,H.R., Myoung,J., Ganem,D., Oh,T.K., Kim,J.F.,
            Gao,S.J. and Jung,J.U.
  TITLE     Construction and manipulation of a new Kaposi's sarcoma-associated
            herpesvirus Bacterial artificial chromosome clone
  JOURNAL   J. Virol. 86 (18), 9708-9720 (2012)
   PUBMED   22740391
REFERENCE   2  (bases 1 to 138146)
  AUTHORS   Brulois,K., Lee,A. and Chang,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2009) Molecular Microbiology and Immunology,
            University of Southern California, 1450 Biggy Street, Los Angeles,
            CA 90033, USA
FEATURES             Location/Qualifiers
     Site            1..6
                     /site_type="restriction site"
                     /label=BamHI
     source          <1..>1093
                     /organism="Human herpesvirus 8"
                     /mol_type="genomic DNA"
                     /strain="JSC-1"
                     /db_xref="taxon:37296"
                     /clone="BAC16"
                     /note="infectious BAC clone harboring full-length KSHV
                     genome; derived from rKSHV.219, KSHV-infected JSC1
                     primary effusion lymphoma cell line"
     Site            1..6
                     /site_type="mutagenized site"
                     /label="5' RE site"
     misc_RNA        complement(<7..1069)
                     /note="5' end approx."
                     /note="corresp. pos."
                     /label="K7.3 (anti-PAN lncRNA) - Chang/Moore lab 2011"
     misc_RNA        <7..1086
                     /note="5' end not determine, est. based on Fig. 2"
                     /label="K4.5 (T6.1, as-K5-K6) - Ganem lab 2014"
     misc_RNA        7..1087
                     /note="corresp. pos."
                     /label="PAN RNA - Zheng lab 2013"
     misc_RNA        <7..1087
                     /note="corresp. to 5' end ID'd by 2005 Taylor et al.
                     (Chang/Moore lab)"
                     /note="corresp. to PAN RNA 3' end ID'd by KSHV 2.0"
                     /note="corresp. pos."
                     /label="K4.5 (T6.1) - Chang/Moore lab 2005/2011, Ganem
                     lab 2014"
     CDS             <7..348
                     /codon_start=1
                     /product="K7"
                     /protein_id="ACY00412.1"
                     /db_xref="GI:261853490"
                     /translation="MGTLEIKGASLSQFSTGTAQSPWLPLHLWILCSLLAFLPLLVFI
                     GAADCGLIASLLAIYPSWLSARFSVLLFPHWPESCSTKNTARSGALHKPAEQKLRFAQ
                     KPCHGNYTVTPCGLLHWIQSPGQL"
     misc_RNA        12..1086
                     /note="from 2014 Arias et al. (Ganem lab) KSHV 2.0"
                     /label="PAN RNA - Ganem lab 2014"
     misc_RNA        13..>1087
                     /note="corresp. pos."
                     /label="PAN RNA - NC_009333 genome annotation"
     polyA_site      271
                     /note="corresp. pos."
                     /label="TTS K4.5(T6.1)-PAN - Zheng lab 2013"
     polyA_site      623
                     /note="corresp. pos."
                     /label="TTS K4.5(T6.1)-PAN-K7 - Zheng lab 2013"
     polyA_signal    1065..1070
                     /note="corresp. pos."
                     /label="PAS K4.5-PAN-K7 - Gao lab 2014"
     polyA_site      1085
                     /note="corresp. pos."
                     /label="TTS K4.5(T6.1)-PAN-K7 - Zheng lab 2013"
     Site            1088..1093
                     /site_type="restriction site"
                     /label=EcoRI
     Site            1088..1093
                     /site_type="mutagenized site"
                     /label="3' RE site"
ORIGIN
        1 ggatccTTTA GCACTGGGAC TGCCCAGTCA CCTTGGCTGC CGCTTCACCT ATGGATTTTG
       61 TGCTCGCTGC TTGCCTTCTT GCCGCTTCTG GTTTTCATTG GTGCCGCCGA TTGTGGGTTG
      121 ATTGCGTCGC TTTTGGCAAT ATACCCATCC TGGCTTTCGG CTAGGTTTTC CGTCCTACTT
      181 TTCCCACATT GGCCTGAGAG CTGTAGTACA AAAAACACCG CGCGGTCTGG AGCTCTCCAT
      241 AAGCCCGCAG AACAAAAGCT GCGATTTGCC CAAAAACCTT GCCATGGCAA CTATACAGTC
      301 ACCCCTTGCG GGTTATTGCA TTGGATTCAA TCTCCAGGCC AGTTGTAGCC CCCTTTTATG
      361 ATATGCGAGG ATACTTAACG TGTCTGAATG TGGAATATAA TGTGAAAGGA AAGCAGCGCC
      421 CACTGGTGTA TCAGAACAGT GGTGCACTAC CTATCTGCTC ATTCGTTGTT TCGGTTCTGT
      481 GTTTGTCTGA TTCTTAGATA GTGTTGAGGT AATTCTAGAA AGCGGATTGA GTGTAAATCG
      541 GGCCACTTTG CCCTAAATGT GACAATCTGG ATGTGTATCT TATTGGTGCG TTGTGAAGCA
      601 TTTTAAAATG CGTTTTAGAT TGTATCAGGC TAGTGCTGTA ATGGTGTGTT TATTTTTCCA
      661 GTGTAAGCAA GTCGATTTGA ATGACATAGG CGACAAAGTG AGGTGGCATT TGTCAGAAGT
      721 TTCAAAGTCG TGTAAGAACA TTGGACTAAA GTGGTGTGCG GCAGCTGGGA GCGCTCTTTC
      781 AATGTTAATG TTTTAATGTG TATGTTGTGT TGGAAGTTCC AGGCTAATAT TTGATGTTTT
      841 GCTAGGTTGA CTAACGATGT TTTCTTGTAG GTGAAAGCGT TGTGTAACAA TGATAACGGT
      901 GTTTTGGCTG GGTTTTTCCT TGTTCGCACC GGACACCTCC AGTGACCAGA CGGCAAGGTT
      961 TTTATCCCAG TGTATATTGG AAAAACATGT TATACTTTTG ACAATTTAAC GTGCCTAGAG
     1021 CTCAAATTAA ACTAATACCA TAACGTAATG CAACTTACAA CATAAATAAA GGTCAATGTT
     1081 TAATCCAgaa ttc
//