LOCUS pOME0244P 1093 bp DNA linear VRL 27-AUG-2012 DEFINITION PAN-RNA Human herpesvirus 8 strain JSC-1 clone BAC16, complete genome. ACCESSION GQ994935 VERSION GQ994935.1 GI:261853473 KEYWORDS . SOURCE Human herpesvirus 8 (Kaposi's sarcoma-associated herpesvirus) ORGANISM Human herpesvirus 8 Viruses; dsDNA viruses, no RNA stage; Herpesvirales; Herpesviridae; Gammaherpesvirinae; Rhadinovirus. REFERENCE 1 (bases 1 to 138146) AUTHORS Brulois,K.F., Chang,H., Lee,A.S., Ensser,A., Wong,L.Y., Toth,Z., Lee,S.H., Lee,H.R., Myoung,J., Ganem,D., Oh,T.K., Kim,J.F., Gao,S.J. and Jung,J.U. TITLE Construction and manipulation of a new Kaposi's sarcoma-associated herpesvirus Bacterial artificial chromosome clone JOURNAL J. Virol. 86 (18), 9708-9720 (2012) PUBMED 22740391 REFERENCE 2 (bases 1 to 138146) AUTHORS Brulois,K., Lee,A. and Chang,H. TITLE Direct Submission JOURNAL Submitted (21-SEP-2009) Molecular Microbiology and Immunology, University of Southern California, 1450 Biggy Street, Los Angeles, CA 90033, USA FEATURES Location/Qualifiers Site 1..6 /site_type="restriction site" /label=BamHI source <1..>1093 /organism="Human herpesvirus 8" /mol_type="genomic DNA" /strain="JSC-1" /db_xref="taxon:37296" /clone="BAC16" /note="infectious BAC clone harboring full-length KSHV genome; derived from rKSHV.219, KSHV-infected JSC1 primary effusion lymphoma cell line" Site 1..6 /site_type="mutagenized site" /label="5' RE site" misc_RNA complement(<7..1069) /note="5' end approx." /note="corresp. pos." /label="K7.3 (anti-PAN lncRNA) - Chang/Moore lab 2011" misc_RNA <7..1086 /note="5' end not determine, est. based on Fig. 2" /label="K4.5 (T6.1, as-K5-K6) - Ganem lab 2014" misc_RNA 7..1087 /note="corresp. pos." /label="PAN RNA - Zheng lab 2013" misc_RNA <7..1087 /note="corresp. to 5' end ID'd by 2005 Taylor et al. (Chang/Moore lab)" /note="corresp. to PAN RNA 3' end ID'd by KSHV 2.0" /note="corresp. pos." /label="K4.5 (T6.1) - Chang/Moore lab 2005/2011, Ganem lab 2014" CDS <7..348 /codon_start=1 /product="K7" /protein_id="ACY00412.1" /db_xref="GI:261853490" /translation="MGTLEIKGASLSQFSTGTAQSPWLPLHLWILCSLLAFLPLLVFI GAADCGLIASLLAIYPSWLSARFSVLLFPHWPESCSTKNTARSGALHKPAEQKLRFAQ KPCHGNYTVTPCGLLHWIQSPGQL" misc_RNA 12..1086 /note="from 2014 Arias et al. (Ganem lab) KSHV 2.0" /label="PAN RNA - Ganem lab 2014" misc_RNA 13..>1087 /note="corresp. pos." /label="PAN RNA - NC_009333 genome annotation" polyA_site 271 /note="corresp. pos." /label="TTS K4.5(T6.1)-PAN - Zheng lab 2013" polyA_site 623 /note="corresp. pos." /label="TTS K4.5(T6.1)-PAN-K7 - Zheng lab 2013" polyA_signal 1065..1070 /note="corresp. pos." /label="PAS K4.5-PAN-K7 - Gao lab 2014" polyA_site 1085 /note="corresp. pos." /label="TTS K4.5(T6.1)-PAN-K7 - Zheng lab 2013" Site 1088..1093 /site_type="restriction site" /label=EcoRI Site 1088..1093 /site_type="mutagenized site" /label="3' RE site" ORIGIN 1 ggatccTTTA GCACTGGGAC TGCCCAGTCA CCTTGGCTGC CGCTTCACCT ATGGATTTTG 61 TGCTCGCTGC TTGCCTTCTT GCCGCTTCTG GTTTTCATTG GTGCCGCCGA TTGTGGGTTG 121 ATTGCGTCGC TTTTGGCAAT ATACCCATCC TGGCTTTCGG CTAGGTTTTC CGTCCTACTT 181 TTCCCACATT GGCCTGAGAG CTGTAGTACA AAAAACACCG CGCGGTCTGG AGCTCTCCAT 241 AAGCCCGCAG AACAAAAGCT GCGATTTGCC CAAAAACCTT GCCATGGCAA CTATACAGTC 301 ACCCCTTGCG GGTTATTGCA TTGGATTCAA TCTCCAGGCC AGTTGTAGCC CCCTTTTATG 361 ATATGCGAGG ATACTTAACG TGTCTGAATG TGGAATATAA TGTGAAAGGA AAGCAGCGCC 421 CACTGGTGTA TCAGAACAGT GGTGCACTAC CTATCTGCTC ATTCGTTGTT TCGGTTCTGT 481 GTTTGTCTGA TTCTTAGATA GTGTTGAGGT AATTCTAGAA AGCGGATTGA GTGTAAATCG 541 GGCCACTTTG CCCTAAATGT GACAATCTGG ATGTGTATCT TATTGGTGCG TTGTGAAGCA 601 TTTTAAAATG CGTTTTAGAT TGTATCAGGC TAGTGCTGTA ATGGTGTGTT TATTTTTCCA 661 GTGTAAGCAA GTCGATTTGA ATGACATAGG CGACAAAGTG AGGTGGCATT TGTCAGAAGT 721 TTCAAAGTCG TGTAAGAACA TTGGACTAAA GTGGTGTGCG GCAGCTGGGA GCGCTCTTTC 781 AATGTTAATG TTTTAATGTG TATGTTGTGT TGGAAGTTCC AGGCTAATAT TTGATGTTTT 841 GCTAGGTTGA CTAACGATGT TTTCTTGTAG GTGAAAGCGT TGTGTAACAA TGATAACGGT 901 GTTTTGGCTG GGTTTTTCCT TGTTCGCACC GGACACCTCC AGTGACCAGA CGGCAAGGTT 961 TTTATCCCAG TGTATATTGG AAAAACATGT TATACTTTTG ACAATTTAAC GTGCCTAGAG 1021 CTCAAATTAA ACTAATACCA TAACGTAATG CAACTTACAA CATAAATAAA GGTCAATGTT 1081 TAATCCAgaa ttc //