LOCUS       pOME0245M      1099 bp    DNA     linear   VRL 27-AUG-2012
DEFINITION  Reverse compl PAN-RNA pOME0244 Human herpesvirus 8 strain JSC-1 clone BAC16, complete genome.
ACCESSION   GQ994935
VERSION     GQ994935.1  GI:261853473
KEYWORDS    .
SOURCE      Human herpesvirus 8 (Kaposi's sarcoma-associated herpesvirus)
  ORGANISM  Human herpesvirus 8
            Viruses; dsDNA viruses, no RNA stage; Herpesvirales; Herpesviridae;
            Gammaherpesvirinae; Rhadinovirus.
REFERENCE   1  (bases 1 to 138146)
  AUTHORS   Brulois,K.F., Chang,H., Lee,A.S., Ensser,A., Wong,L.Y., Toth,Z.,
            Lee,S.H., Lee,H.R., Myoung,J., Ganem,D., Oh,T.K., Kim,J.F.,
            Gao,S.J. and Jung,J.U.
  TITLE     Construction and manipulation of a new Kaposi's sarcoma-associated
            herpesvirus Bacterial artificial chromosome clone
  JOURNAL   J. Virol. 86 (18), 9708-9720 (2012)
   PUBMED   22740391
REFERENCE   2  (bases 1 to 138146)
  AUTHORS   Brulois,K., Lee,A. and Chang,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2009) Molecular Microbiology and Immunology,
            University of Southern California, 1450 Biggy Street, Los Angeles,
            CA 90033, USA
COMMENT     Reverse of pOME0244 to serve as negative control
FEATURES             Location/Qualifiers
     misc_RNA        1..>1099
                     /note="5' end not determine, est. based on Fig. 2"
                     /label="K4.5 (T6.1, as-K5-K6) - Ganem lab 2014"
     misc_RNA        complement(1..>1099)
                     /note="5' end approx."
                     /note="corresp. pos."
                     /label="K7.3 (anti-PAN lncRNA) - Chang/Moore lab 2011"
     misc_RNA        1..>1099
                     /note="corresp. to 5' end ID'd by 2005 Taylor et al.
                     (Chang/Moore lab)"
                     /note="corresp. to PAN RNA 3' end ID'd by KSHV 2.0"
                     /note="corresp. pos."
                     /label="K4.5 (T6.1) - Chang/Moore lab 2005/2011, Ganem
                     lab 2014"
     misc_RNA        1..1099
                     /note="corresp. pos."
                     /label="PAN RNA - Zheng lab 2013"
     Site            1..6
                     /site_type="restriction site"
                     /label=EcoRI
     source          <1..>1099
                     /organism="Human herpesvirus 8"
                     /mol_type="genomic DNA"
                     /strain="JSC-1"
                     /db_xref="taxon:37296"
                     /clone="BAC16"
                     /note="infectious BAC clone harboring full-length KSHV
                     genome; derived from rKSHV.219, KSHV-infected JSC1
                     primary effusion lymphoma cell line"
     Site            1..6
                     /site_type="mutagenized site"
                     /label="3' RE site"
     CDS             7..>1087
                     /cds_type=ORF
                     /codon_start=1
                     /product="K7"
                     /protein_id="ACY00412.1"
                     /db_xref="GI:261853490"
                     /translation="MGTLEIKGASLSQFSTGTAQSPWLPLHLWILCSLLAFLPLLVFI
                     GAADCGLIASLLAIYPSWLSARFSVLLFPHWPESCSTKNTARSGALHKPAEQKLRFAQ
                     KPCHGNYTVTPCGLLHWIQSPGQL"
     Site            1088..1093
                     /site_type="restriction site"
                     /label=BamHI
     Site            1088..1093
                     /site_type="mutagenized site"
                     /label="5' RE site"
ORIGIN
        1 gaattcTGGA TTAAACATTG ACCTTTATTT ATGTTGTAAG TTGCATTACG TTATGGTATT
       61 AGTTTAATTT GAGCTCTAGG CACGTTAAAT TGTCAAAAGT ATAACATGTT TTTCCAATAT
      121 ACACTGGGAT AAAAACCTTG CCGTCTGGTC ACTGGAGGTG TCCGGTGCGA ACAAGGAAAA
      181 ACCCAGCCAA AACACCGTTA TCATTGTTAC ACAACGCTTT CACCTACAAG AAAACATCGT
      241 TAGTCAACCT AGCAAAACAT CAAATATTAG CCTGGAACTT CCAACACAAC ATACACATTA
      301 AAACATTAAC ATTGAAAGAG CGCTCCCAGC TGCCGCACAC CACTTTAGTC CAATGTTCTT
      361 ACACGACTTT GAAACTTCTG ACAAATGCCA CCTCACTTTG TCGCCTATGT CATTCAAATC
      421 GACTTGCTTA CACTGGAAAA ATAAACACAC CATTACAGCA CTAGCCTGAT ACAATCTAAA
      481 ACGCATTTTA AAATGCTTCA CAACGCACCA ATAAGATACA CATCCAGATT GTCACATTTA
      541 GGGCAAAGTG GCCCGATTTA CACTCAATCC GCTTTCTAGA ATTACCTCAA CACTATCTAA
      601 GAATCAGACA AACACAGAAC CGAAACAACG AATGAGCAGA TAGGTAGTGC ACCACTGTTC
      661 TGATACACCA GTGGGCGCTG CTTTCCTTTC ACATTATATT CCACATTCAG ACACGTTAAG
      721 TATCCTCGCA TATCATAAAA GGGGGCTACA ACTGGCCTGG AGATTGAATC CAATGCAATA
      781 ACCCGCAAGG GGTGACTGTA TAGTTGCCAT GGCAAGGTTT TTGGGCAAAT CGCAGCTTTT
      841 GTTCTGCGGG CTTATGGAGA GCTCCAGACC GCGCGGTGTT TTTTGTACTA CAGCTCTCAG
      901 GCCAATGTGG GAAAAGTAGG ACGGAAAACC TAGCCGAAAG CCAGGATGGG TATATTGCCA
      961 AAAGCGACGC AATCAACCCA CAATCGGCGG CACCAATGAA AACCAGAAGC GGCAAGAAGG
     1021 CAAGCAGCGA GCACAAAATC CATAGGTGAA GCGGCAGCCA AGGTGACTGG GCAGTCCCAG
     1081 TGCTAAAgga tccgagctc
//