LOCUS pOME0247M 6166 bp DNA linear VRL 27-AUG-2012 DEFINITION Reverse T61 Human herpesvirus 8 strain JSC-1 clone BAC16, complete genome. ACCESSION GQ994935 VERSION GQ994935.1 GI:261853473 KEYWORDS . SOURCE Human herpesvirus 8 (Kaposi's sarcoma-associated herpesvirus) ORGANISM Human herpesvirus 8 Viruses; dsDNA viruses, no RNA stage; Herpesvirales; Herpesviridae; Gammaherpesvirinae; Rhadinovirus. REFERENCE 1 (bases 1 to 138146) AUTHORS Brulois,K.F., Chang,H., Lee,A.S., Ensser,A., Wong,L.Y., Toth,Z., Lee,S.H., Lee,H.R., Myoung,J., Ganem,D., Oh,T.K., Kim,J.F., Gao,S.J. and Jung,J.U. TITLE Construction and manipulation of a new Kaposi's sarcoma-associated herpesvirus Bacterial artificial chromosome clone JOURNAL J. Virol. 86 (18), 9708-9720 (2012) PUBMED 22740391 REFERENCE 2 (bases 1 to 138146) AUTHORS Brulois,K., Lee,A. and Chang,H. TITLE Direct Submission JOURNAL Submitted (21-SEP-2009) Molecular Microbiology and Immunology, University of Southern California, 1450 Biggy Street, Los Angeles, CA 90033, USA FEATURES Location/Qualifiers Site 1..6 /site_type="restriction site" /label=EcoRI repeat_region 1..>6166 /rpt_family="LIR1" /rpt_type=inverted source <1..>6166 /organism="Human herpesvirus 8" /mol_type="genomic DNA" /strain="JSC-1" /db_xref="taxon:37296" /clone="BAC16" /note="infectious BAC clone harboring full-length KSHV genome; derived from rKSHV.219, KSHV-infected JSC1 primary effusion lymphoma cell line" Site 1..6 /site_type="mutagenized site" /label="5' RE" misc_RNA complement(7..6155) /note="corresp. to 5' end ID'd by 2005 Taylor et al. (Chang/Moore lab)" /note="corresp. to PAN RNA 3' end ID'd by KSHV 2.0" /note="corresp. pos." /label="K4.5 (T6.1) - Chang/Moore lab 2005/2011, Ganem lab 2014" /label="Reverse complimentK4.5 (T6.1) - Chang/Moore lab 2005/2011, Ganem lab 2014" Site 6156..6161 /site_type="restriction site" /label=KpnI Site 6156..6166 /site_type="mutagenized site" /label="3' RE site" ORIGIN 1 gaattcGGAT TAAACATTGA CCTTTATTTA TGTTGTAAGT TGCATTACGT TATGGTATTA 61 GTTTAATTTG AGCTCTAGGC ACGTTAAATT GTCAAAAGTA TAACATGTTT TTCCAATATA 121 CACTGGGATA AAAACCTTGC CGTCTGGTCA CTGGAGGTGT CCGGTGCGAA CAAGGAAAAA 181 CCCAGCCAAA ACACCGTTAT CATTGTTACA CAACGCTTTC ACCTACAAGA AAACATCGTT 241 AGTCAACCTA GCAAAACATC AAATATTAGC CTGGAACTTC CAACACAACA TACACATTAA 301 AACATTAACA TTGAAAGAGC GCTCCCAGCT GCCGCACACC ACTTTAGTCC AATGTTCTTA 361 CACGACTTTG AAACTTCTGA CAAATGCCAC CTCACTTTGT CGCCTATGTC ATTCAAATCG 421 ACTTGCTTAC ACTGGAAAAA TAAACACACC ATTACAGCAC TAGCCTGATA CAATCTAAAA 481 CGCATTTTAA AATGCTTCAC AACGCACCAA TAAGATACAC ATCCAGATTG TCACATTTAG 541 GGCAAAGTGG CCCGATTTAC ACTCAATCCG CTTTCTAGAA TTACCTCAAC ACTATCTAAG 601 AATCAGACAA ACACAGAACC GAAACAACGA ATGAGCAGAT AGGTAGTGCA CCACTGTTCT 661 GATACACCAG TGGGCGCTGC TTTCCTTTCA CATTATATTC CACATTCAGA CACGTTAAGT 721 ATCCTCGCAT ATCATAAAAG GGGGCTACAA CTGGCCTGGA GATTGAATCC AATGCAATAA 781 CCCGCAAGGG GTGACTGTAT AGTTGCCATG GCAAGGTTTT TGGGCAAATC GCAGCTTTTG 841 TTCTGCGGGC TTATGGAGAG CTCCAGACCG CGCGGTGTTT TTTGTACTAC AGCTCTCAGG 901 CCAATGTGGG AAAAGTAGGA CGGAAAACCT AGCCGAAAGC CAGGATGGGT ATATTGCCAA 961 AAGCGACGCA ATCAACCCAC AATCGGCGGC ACCAATGAAA ACCAGAAGCG GCAAGAAGGC 1021 AAGCAGCGAG CACAAAATCC ATAGGTGAAG CGGCAGCCAA GGTGACTGGG CAGTCCCAGT 1081 GCTAAACTGA CTCAAGCTGG CCCCTTTTAT CTCCAGTGTT CCCATATTTT GGACAGGTTA 1141 GCCACCCATT TTTGGAAGCC ACGCCCCTTT ATGTCATTAA CATCATCCTT CAAGCTGACC 1201 CTTAATAATC CATAAGAAAA CTCCATACTC CTACCAAATA TGGAAACCAA ATTACATATA 1261 CACAATACGG ATGAACATTA ATAAACTTTC ATTAATGTTC ATCCAGTCCC CCAAATCTAC 1321 CGAATCACTA TGTTGGGTCA CCCCCCGCCC AGTTATTCAC GGGGTAATCG AGCAGAGTAG 1381 CCAAGCTGCG AATACTTCCG CAACCAGTCG GGATAAGGAA TATAATATGT AGATAACCCC 1441 AAAACAAACC CAAACACGAC CATTGGATAA TTTGCTTCTA CCAACCCGGC CACGCGATTT 1501 TAGCTGCGCT GCAAATCGCT GGCGGACGTG TGTGATTGTC GGACTCGTCT ACCTATCAGT 1561 AACCGTGGTC AAACCCGCCA ACACGGCATT GGCCGAAGAC GTGTGCTTCT GAGCGGCGAT 1621 GACAGGTGAT TGACTGGTTG GTTGACAAAT AAAAAAAGAC TATGCGTTTA GAGGGTATTA 1681 ACCAACTCAC GATGGCGTTG CTCACAACCG TGCCAAAAAA ATATATAATT ATTTCGGCTC 1741 GCCCACGGTC CCACCTAGCG GCGTGCACTG GAGGTGACCC CTGACTTGGT CGTCGGTATG 1801 TTGTCTCCGC GCAAGCGCAC AATACAGATA TGCACGATAC ATGCAGTCTC CGGTGACACA 1861 TACAGAGACA CGCCTCAAGC AGCGGTGTTG ATGGGTCGTG GGAATATGGT ACACTGATCG 1921 CCAGATGACC CCTGAAACTA CCCGGCTTGT TTTCGCCCGG GTGGCAATGG AAACCTGGCT 1981 TAATGACAGA ACTCAAAGCC CACAAAGGCG CCACAGCGAC GCCGGCGCAG GCCGCACCGA 2041 CTGGAACGCA TGCGGTTTCC GGGCGTTGGC GGGGTTTCTT AGAAACCCTC ACACCTTCTA 2101 AGTAAGCGCC CCAACAATCA CTTTTGTCTG CAACCCGTTT TTGGTGAGTT AGGCCAACTT 2161 ATCCCGCATG TAAGCTGGCG GTTAGCATAC GCCACTATAA ATAAACAGGT GAGACCAGAA 2221 TCAGGTAAGC ATATAAGGAA CTCGGCGTTA CATCTGACAG AACAGAAACC CCAAATAGCC 2281 TTTACGGTTT TCTTTAGACT GTTGCGAACT TGGCACCTCC AGATGGCCCC CGTCCACGTT 2341 TTATGCTGCG TTAGCGTACT GCTTGCCACG TTTTACCTGA CGCCCACAGA AAGCGCGGGG 2401 TCACTCGTGT CGTACACGCC TAATAGCTGC TGCTACGGGT TCCAGCAGCA CCCACCGCCC 2461 GTCCAAATTC TAAAAGAGTG GTATCCCACG TCCCCAGCGT GCCCAAAACC CGGAGTTATT 2521 TTGCTGACCA AGCGAGGGCG TCAGATCTGC GCAGACCCTT CCAAAAACTG GGTTAGGCAG 2581 CTGATGCAGC GGCTGCCTGC CATAGCTTAG ACGGTCGAAA GTTGAAGATA TCACTGCGGC 2641 CCAACTGCTC GAGGCGACAA CGCCATCGTG CGCCTAGGCC ATTCTAGGAG ACGCGCAAAG 2701 GGGGGGGATT TATCTACCTT AGAAGACCTG TCGAGGAAGA AATGGGGGAA AAACGTTTTT 2761 CCCAGAAGCG CACCACAGAA ACGCCTGTCG TACGCGGGGG GTGGGGGATC CACTCAGGTG 2821 TTTTCCAATA AAGCTGTTGT GGTTTTTCTC TCATAACTAG TGATGACGCG TGTCCCTTAT 2881 TTCATAGGTC CGGTGTGGGC GGATCCGGAG GGAAGGAAGG GCGGGGCGCG CCGTGTTTTG 2941 GACAACTTGT CCGGAGCTGC CTAGAACTAA CCACTCATAA AACCTCCAAA CACAGGCGGA 3001 GAGCGCGGGG GTGGACGGGC ACGAAGCGTT GATCCTGTGC GTAAACAAAA ATACAGATGT 3061 AGAGCTAAAG CAGGGTGGGT TGTGACAGTT TTCCAGGGAA AGCCTGGATG TAGGAAAATA 3121 GACACACATG TGGCGTCCGT GAATATGTTA GACATCTGTC AAGGCGGGAT AGCGACAGTA 3181 GGGCGGACGG CGGGTCGGAG ACTAACTGGG CACTCTGCTC ACCTCCCCTT TCCCTTTTTC 3241 AGACTTCCAC CCCAGCTGCA GAGATGGCGT CCAAGGACGT AGAAGAGGGT GTAGAGGGAC 3301 CCATCTGCTG GATATGTAGG GAAGAGGTGG GGAACGAGGG CATACACCCC TGCGCCTGTA 3361 CCGGAGAGCT GGATGTCGTC CACCCGCAGT GTTTAAGCAC TTGGCTAACA GTGTCTCGAA 3421 ACACGGCCTG TCAAATGTGT CGCGTTATAT ACCGCACGCG CACGCAGTGG CGTAGTCGCC 3481 TTAACCTGTG GCCGGAGATG GAGCGCCAAG AAATTTTTGA ACTGTTTTTG CTGATGTCTG 3541 TGGTGGTGGC TGGGCTTGTC GGCGTGGCGT TGTGCACCTG GACGCTCCTG GTCATCCTAA 3601 CTGCTCCTGC GGGAACATTC TCCCCCGGGG CCGTGCTCGG TTTTCTCTGC TTCTTTGGGT 3661 TTTACCAAAT ATTTATTGTG TTTGCATTTG GCGGCATATG CCGCGTAAGT GGCACTGTGA 3721 GGGCATTATA CGCGGCAAAT AACACCCGGG TGACCGTACT GCCATACCGA CGGCCGCGCC 3781 GGCCAACCGC GAACGAAGAT AACATCGAAT TGACGGTCCT TGTCGGACCC GCGGGCGGGA 3841 CGGACGAGGA GCCCACGGAC GAGTCATCTG AAGGAGACGT CGCCTCTGGA GACAAAGAAC 3901 GTGACGGTTC ATCCGGAGAC GAGCCGGACG GGGGCCCGAA CGACCGTGCG GGACTTAGGG 3961 GGACAGCGCG TACCGACCTA TGCGCGCCCA CAAAAAAGCC GGTGCGGAAA AATCATCCAA 4021 AAAACAACGG TTGAACAACC ACTTGGGTGC ACAGAGATAT GTGACGTGAC GCCCTGGGTG 4081 TTATGCACCG TGCAGGTTTT CGCAGCGTAT ACACCCCCCC CGCGCACACG TAGGGCTTTA 4141 CCCTCTGGCC TGTGCCCAAA TCTGTCCTCA TTAAAAACAA GTAGCTGTTG ACTTTCAAAC 4201 AACCCGCGTG CGATATGTTT TCCATCCGTG TCCTCTAGTT TTCGGGTGAT TAAGCAAAGG 4261 GGGGATGGGT GCAACGACAA ATGAGGGTGA GTGGAACCTT TTACTCGGTA AACATTGTTT 4321 ATTTCAAGAG CCTATGCTGG GACAGAATAG CCGGGGAAAA GTGGACGGAG AATAGACCAG 4381 CCTGAAACGT CGCCCAAATC GTAGGCCAAT ACTGCCCAAG TATGGAATGG TGACTAAGAA 4441 AAACTCTCTC TAAACAATGT GAATCCTCTG TGCCCCCTTT GAATAAATGC TGGAACGGAT 4501 GATTCTTATT TCCGTTCGCT GGAAGCGGGA TATGCTTCCG CCTCATCAAA TACCTATACT 4561 ACATATACGA CACGCCTTGT GTACAGTGGG TGACACTAAC ACGGTGACGT GCTACCTGTG 4621 TAAATTTCGG TGTGGTTTTT AATGATATTG GATAACGATG TTTTTAAAGT TTTTTGACGC 4681 GTGGCTGTGC TTCTCATCAT GGTGCATTTG GGGGTTATAT GCGCGTGCTT GCGACGGATG 4741 CGGTCCGCCG TACGTGTAGA ACGTTTTAAA CACCATTAAT ACTAGCAGGG GTTGTGTGCT 4801 TGTGACTGAT ACAAATCGCC ATACATGGCA ACAGGTTGTT GCCGGGGAGG GTGGCGCGCC 4861 GGGATCCCCC TCCGGGGGAG GGTGGCGCGC CGGGATCCCC CTCCGGGGGA GGGTGGCGCG 4921 CCGGGATCCC CCTCCGGGGG AGGGTGGCGC GCCGGGATCC CCCTCCGGGG GAGGGTGGCG 4981 CGCCGGGATC CCCCTCCGGG GGGGGGGTGG GGTGCTGCCG GGGCTCCTGG GGTGCTGCCG 5041 GGGCTCCTGG GGTGCTGCCG GGGCTCCTGG GGTCCTGCCG GGGCTCCTGG GGTGCTGCCG 5101 GGGCTCCTGG GGTGCTGCCG GGGCTCCTGG GGTGCTGCCG GGGCTCCTGG GGTGCTGCCG 5161 GGGCTCCTGG GGTGCTGCCG GGGCTCCTGG GGTGCTGCCG GGGCTCCTGG GGTGCTGCCG 5221 GGGCTCCTGG GGTGCTGCCG GGGCTCCTGG GGTGCTGCCG GGGCTCCTGG GGTGCTGCCG 5281 GGGCTCCTGG GGTGCTGCCG GGGCTCCTGG GGTGCTGCCG GGGCTCCTGG GGTGCTGCCG 5341 GGGCTCCTGG GGTGCTGCCG GGGCTCCTGG GGTGCTGCCG GGGCTCCTGG GGTGCTGCCG 5401 GGGCTCCTGG GGTGCTGCCG GGGCTCCTGG GGTGCTGCCG GGGCTCCTGG GGTGCGCCGC 5461 TCCTCGTTAC GGGTAAATCC AAGAGATCCG TCCCACGTGC CGCCGCGCGC GCAGTGCGCG 5521 AGGGGGGCTG CTGTCCCTCG TTTTTATAGC GTTCCCGGCG AAGGGGTGTA ACAGTTGGTT 5581 AACCCGTCTC AAATGGGCGT AACCGTAGGA CAAGCTGCCC ATGTAGGCGT TAGCCACCCA 5641 TGTTCTGGGG CGCGCCAGCG GTGGGCGGGG CCCCATCCTC CCGGCCCCCA TCTACCGAAA 5701 CCACCCAGAG GCGAGATTCC AGGCCCGTGA CTCACTAGCC CCCCCCCCCC CGCCACCGAA 5761 CAACCCCGTG GACAGGGCCC CTATAATCCT GGCACCCATC CACCGAAACG ACCTAGTAGA 5821 AAAATCCCAA CCCCATGACT CATCGGGCCC TATTATAAAG AATAACCCAG TAGAGTGACC 5881 CAGCTGATTT CCATAAATGG ATATACTTCC GGAAAACAAA GGAGGGTTGA ATACAGTTGG 5941 GGGTAGTCCG CTGGTATTCC CAGCTGAGGT TGCCTTATTT GGTAATGCTT CCGGAAATAC 6001 CACCTGAGGA CCCCGTTGGT TTCTACCTTG TTTAATTATA GAATTGCAGC TGGGTATACC 6061 CAGCTGGGTT CACCCACCTG GGTATACCTA CTGGATTACC CAGCTGGGTA TACCTACTGG 6121 AATAGGGGCT GCGATGACTC AGCTGCGCTA GGATTggtac cgtacc //