LOCUS       pOME0252       395 bp    DNA     linear   UNA 26-May-2015
DEFINITION  5' UTR negative strand ORF CHIKV
FEATURES             Location/Qualifiers
     Site            1..6
                     /site_type="restriction site"
                     /label=KpnI
     Site            7..12
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             13..381
                     /cds_type=ORF
                     /note="Length: 300"
                     /note="Found at strand: positive"
                     /note="Start codon: atg"
     Site            310..375
                     /site_type="mutagenized site"
                     /label="3X FLAG tag"
     Site            376..381
                     /site_type="mutagenized site"
                     /label="double stop"
     Site            382..387
                     /site_type="restriction site"
                     /label=XhoI
     Site            388..395
                     /site_type="restriction site"
                     /label=NotI
ORIGIN
        1 ggtaccgcca ccatgTTAGC GAGTCTCTCG GGATCTTCCG CACTGCGCAT CGGGCAGACG
       61 CAGTGGTACT TCCTGTCCGA CATCATCCTC CTTGCTGGCG CACTGCCGAT ATCCAGGATG
      121 GTTGAGTCGG GGTCAATTTC CTGCTCTATT AGTTTTATAG CTAGATGCGA GAACGCTCTA
      181 GCATTAGCAT GGTCATTCGG TGTGACCTGC CTTGGTTCCA CCTCAAACAT GGGGTACGCA
      241 CGTTGCAGGG CCTTCAAAAA GGCGCTGTCA GCGTCTATGT CCACGTACAC AGGATCCATG
      301 ATGGGTTATg actacaaaga ccatgacggt gattataaag atcatgatat cgattacaag
      361 gatgacgatg acaagtagta actcgaggcg gccgc
//