LOCUS       pOME0248       452 bp    DNA     linear   UNA 22-May-2015
DEFINITION  Ebola GP-IGR VP30; vector pSIREN
COMMENT     Plasmid synthesized by BioBasic
            Sequence verified via Sanger sequencing through Genewiz
            pOME= plasmid of the Orfeome project
            GP-IGR= glycoprotein intergenic region
            VP= viral protein
FEATURES             Location/Qualifiers
     Site            join(1..6,1^2)
                     /site_type="restriction site"
                     /note="Name: BamHI"
                     /note="Pattern: ggatcc"
                     /note="cut_0_on_positive_strand: 1^2"
                     /note="cut_0_on_negative_strand: 5^6"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=BamHI
     Site            join(447..452,447^448)
                     /site_type="restriction site"
                     /note="Name: EcoRI"
                     /note="Pattern: gaattc"
                     /note="cut_0_on_positive_strand: 447^448"
                     /note="cut_0_on_negative_strand: 451^452"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=EcoRI
ORIGIN
        1 ggatccTTTT TCTTCAGATT GCTTCATGGA AAAGCTCAGC CTCAAATCAA TGAAACCAGG
       61 ATTTAATTAT ATGGATTACT TGAATCTAAG ATTACTTGAC AAATGATAAT ATAATACACT
      121 GGAGCTTTAA ACATAGCCAA TGTGATTCTA ACTCCTTTAA ACTCACAGTT AATCATAAAC
      181 AAGGTTTGAC ATCAATCTAG TTATCTCTTT GAGAATGATA AACTTGATGA AGATTAAGAA
      241 AAAGGTAATC TTTCGATTAT CTTTAATCTT CATCCTTGAT TCTACAATCA TGACAGTTGT
      301 CTTTAGTGAC AAGGGAAAGA AGCCTTTTTA TTAAGTTGTA ATAATCAGAT CTGCGAACCG
      361 GTAGAGTTTA GTTGCAACCT AACACACATA AAGCATTGGT CAAAAAGTCA ATAGAAATTT
      421 AAACAGTGAG TGGAGACAAC TTTTAAgaat tc
//
