LOCUS       pOME0258      1025 bp    DNA     linear   UNA 19-Aug-2015
DEFINITION  
FEATURES             Location/Qualifiers
     Site            1..12
                     /site_type="mutagenized site"
                     /label="5' RE"
     Site            13..18
                     /site_type=modification
                     /note="Addition of 5' SalI/KpnI site and Kozak sequence
                     and 3' XhoI/NotI sites"
                     /label="Kozak sequence"
     Site            13..18
                     /site_type="mutagenized site"
                     /label="Kozak seq"
     CDS             19..1011
                     /cds_type=ORF
                     /note="Length: 924"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     CDS             19..939
                     /cds_type=ORF
                     /label="SARS CoV MA Nsp5"
     Site            940..1005
                     /site_type="mutagenized site"
                     /label="3x FLAG tag"
     Site            1006..1011
                     /site_type="mutagenized site"
                     /label="Double stop"
     Site            1012..1025
                     /site_type="mutagenized site"
                     /label="3' RE"
ORIGIN
        1 gtcgacggta ccgccaccAT GAGTGGTTTT AGGAAAATGG CATTCCCGTC AGGCAAAGTT
       61 GAAGGGTGCA TGGTACAAGT AACCTGTGGA ACTACAACTC TTAATGGATT GTGGTTGGAT
      121 GACACAGTAT ACTGTCCAAG ACATGTCATT TGCACAGCAG AAGACATGCT TAATCCTAAC
      181 TATGAAGATC TGCTCATTCG CAAATCCAAC CATAGCTTTC TTGTTCAGGC TGGCAATGTT
      241 CAACTTCGTG TTATTGGCCA TTCTATGCAA AATTGTCTGC TTAGGCTTAA AGTTGATACT
      301 TCTAACCCTA AGACACCCAA GTATAAATTT GTCCGTATCC AACCTGGTCA AACATTTTCA
      361 GTTCTAGCAT GCTACAATGG TTCACCATCT GGTGTTTATC AGTGTGCCAT GAGACCTAAT
      421 TATACCATTA AAGGTTCTTT CCTTAATGGA TCATGTGGTA GTGTTGGTTT TAACATTGAT
      481 TATGATTGCG TGTCTTTCTG CTATATGCAT CATATGGAGC TTCCAACAGG AGTACACGCT
      541 GGTACTGACT TAGAAGGTAA ATTCTATGGT CCATTTGTTG ACAGACAAAC TGCACAGGCT
      601 GCAGGTACAG ACACAACCAT AACATTAAAT GTTTTGGCAT GGCTGTATGC TGCTGTTATC
      661 AATGGTGATA GGTGGTTTCT TAATAGATTC ACCACTACTT TGAATGACTT TAACCTTGTG
      721 GCAATGAAGT ACAACTATGA ACCTTTGACA CAAGATCATG TTGACATATT GGGACCTCTT
      781 TCTGCTCAAA CAGGAATTGC CGTCTTAGAT ATGTGTGCTG CTTTGAAAGc GCTGCTGCAG
      841 AATGGTATGA ATGGTCGTAC TATCCTTGGT AGCACTATTT TAGAAGATGA GTTTACACCA
      901 TTTGATGTTG TTAGACAATG CTCTGGTGTT ACCTTCCAAg actacaaaga ccatgacggt
      961 gattataaag atcatgatat cgattacaag gatgacgatg acaagtagta actcgaggcg
     1021 gccgc
//