LOCUS pOME0260 2897 bp DNA linear UNA 19-Aug-2015 DEFINITION FEATURES Location/Qualifiers Site 1..6 /site_type="mutagenized site" /note="SalI restriction site" /label="5' RE sites" Site 7..12 /site_type="mutagenized site" /note="" /label="Kozak sequence" CDS 13..2883 /cds_type=ORF /note="Length: 2868" /note="Found at strand: positive" /note="Start codon: ATG" CDS 13..2811 /cds_type=ORF /label="SARS CoV MA Nsp12" Site 2812..2877 /site_type="mutagenized site" /label="3x FLAG tag" Site 2878..2883 /site_type="mutagenized site" /label="double stop" Site 2884..2897 /site_type="mutagenized site" /note="XhoI and NotI restriction sites" /label="3' RE site" ORIGIN 1 gtcgacgcca ccATGTCTGC GGATGCATCA ACGTTTTTAA ACCGGGTTTG CGGTGTAAGT 61 GCAGCCCGTC TTACACCGTG CGGCACAGGC ACTAGTACTG ATGTCGTCTA CAGGGCTTTT 121 GATATTTACA ACGAAAAAGT TGCTGGTTTT GCAAAGTTCC TAAAAACTAA TTGCTGTCGC 181 TTCCAGGAGA AGGATGAGGA AGGCAATTTA TTAGACTCTT ACTTTGTAGT TAAGAGGCAT 241 ACTATGTCTA ACTACCAACA TGAAGAGACT ATTTATAACT TGGTTAAAGA TTGTCCAGCG 301 GTTGCTGTCC ATGACTTTTT CAAGTTTAGA GTAGATGGTG ACATGGTACC ACATATATCA 361 CGTCAGCGTC TAACTAAATA CACAATGGCT GATTTAGTCT ATGCTCTACG TCATTTTGAT 421 GAGGGTAATT GTGATACATT AAAAGAAATA CTCGTCACAT ACAATTGCTG TGATGATGAT 481 TATTTCAATA AGAAGGATTG GTATGACTTC GTAGAGAATC CTGACATCTT ACGCGTATAT 541 GCTAACTTAG GTGAGCGTGT ACGCCAATCA TTATTAAAGA CTGTACAATT CTGCGATGCT 601 ATGCGTGATG CAGGCATTGT AGGCGTACTG ACATTAGATA ATCAGGATCT TAATGGGAAC 661 TGGTACGATT TCGGTGATTT CGTACAAGTA GCACCAGGCT GCGGAGTTCC TATTGTGGAT 721 TCATATTACT CATTGCTGAT GCCCATCCTC ACTTTGACTA GGGCATTGGC TGCTGAGTCC 781 CATATGGATG CTGATCTCGC AAAACCACTT ATTAAGTGGG ATTTGCTGAA ATATGATTTT 841 ACGGAAGAGA GACTTTGTCT CTTCGACCGT TATTTTAAAT ATTGGGACCA GACATACCAT 901 CCCAATTGTA TTAACTGTTT GGATGATAGG TGTATCCTTC ATTGTGCAAA CTTTAATGTG 961 TTATTTTCTA CTGTGTTTCC ACCTACAAGT TTTGGACCAC TAGTAAGAAA AATATTTGTA 1021 GATGGTGTTC CTTTTGTTGT TTCAACTGGA TACCATTTTC GTGAGTTAGG AGTCGTACAT 1081 AATCAGGATG TAAACTTACA TAGCTCGCGT CTCAGTTTCA AGGAACTTTT AGTGTATGCT 1141 GCTGATCCAG CTATGCATGC AGCTTCTGGC AATTTATTGC TAGATAAACG CACTACATGC 1201 TTTTCAGTAG CTGCACTAAC AAACAATGTT GCTTTTCAAA CTGTCAAACC CGGTAATTTT 1261 AATAAAGACT TTTATGACTT TGCTGTGTCT AAAGGTTTCT TTAAGGAAGG AAGTTCTGTT 1321 GAACTAAAAC ACTTCTTCTT TGCTCAGGAT GGCAACGCTG CTATCAGTGA TTATGACTAT 1381 TATCGTTATA ATCTGCCAAC AATGTGTGAT ATCAGACAAC TCCTATTCGT AGTTGAAGTT 1441 GTTGATAAAT ACTTTGATTG TTACGATGGT GGCTGTATTA ATGCCAACCA AGTAATCGTT 1501 AACAATCTGG ATAAATCAGC TGGTTTCCCA TTTAATAAAT GGGGTAAGGC TAGACTTTAT 1561 TATGACTCAA TGAGTTATGA GGATCAAGAT GCACTTTTCG CGTATACTAA GCGTAATGTC 1621 ATCCCTACTA TAACTCAAAT GAATCTTAAG TATGCCATTA GTGCAAAGAA TAGAGCTCGC 1681 ACCGTAGCTG GTGTCTCTAT CTGTAGTACT ATGACAAATA GACAGTTTCA TCAGAAATTA 1741 TTGAAGTCAA TAGCCGCCAC TAGAGGAGCT ACTGTGGTAA TTGGAACAAG CAAGTTTTAC 1801 GGTGGCTGGC ATAATATGTT AAAAACTGTT TACAGTGATG TAGAAACTCC ACACCTTATG 1861 GGTTGGGATT ATCCAAAATG TGACAGAGCC ATGCCTAACA TGCTTAGGAT AATGGCCTCT 1921 CTTGTTCTTG CTCGCAAACA TAACACTTGC TGTAACTTAT CACACCGTTT CTACAGGTTA 1981 GCTAACGAGT GTGCGCAAGT ATTAAGTGAG ATGGTCATGT GTGGCGGCTC ACTATATGTT 2041 AAACCAGGTG GAACATCATC CGGTGATGCT ACAACTGCTT ATGCTAATAG TGTCTTTAAC 2101 ATTTGTCAAG CTGTTACAGC CAATGTAAAT GCACTTCTTT CAACTGATGG TAATAAGATA 2161 GCTGACAAGT ATGTCCGCAA TCTACAACAC AGGCTCTATG AGTGTCTCTA TAGAAATAGG 2221 GATGTTGATC ATGAATTCGT GGATGAGTTT TACGCTTACC TGCGTAAACA TTTCTCCATG 2281 ATGATTCTTT CTGATGATGC CGTTGTGTGC TATAACAGTA ACTATGCGGC TCAAGGTTTA 2341 GTAGCTAGCA TTAAGAACTT TAAGGCAGTT CTTTATTATC AAAATAATGT GTTCATGTCT 2401 GAGGCAAAAT GTTGGACTGA GACTGACCTT ACTAAAGGAC CTCACGAATT TTGCTCACAG 2461 CATACAATGC TAGTTAAACA AGGAGATGAT TACGTGTACC TGCCTTACCC AGATCCATCA 2521 AGAATATTAG GCGCAGGCTG TTTTGTCGAT GATATTGTCA AAACAGATGG TACACTTATG 2581 ATTGAAAGGT TCGTGTCACT GGCTATTGAT GCTTACCCAC TTACAAAACA TCCTAATCAG 2641 GAGTATGCTG ATGTCTTTCA CTTGTATTTA CAATACATTA GAAAGTTACA TGATGAGCTT 2701 ACTGGCCACA TGTTGGACAT GTATTCCGTA ATGCTAACTA ATGATAACAC CTCACGGTAC 2761 TGGGAACCTG AGTTTTATGA GGCTATGTAC ACACCACATA CAGTCTTGCA Ggactacaaa 2821 gaccatgacg gtgattataa agatcatgat atcgattaca aggatgacga tgacaagtag 2881 taactcgagg cggccgc //