LOCUS       pOME0260      2897 bp    DNA     linear   UNA 19-Aug-2015
DEFINITION  
FEATURES             Location/Qualifiers
     Site            1..6
                     /site_type="mutagenized site"
                     /note="SalI restriction site"
                     /label="5' RE sites"
     Site            7..12
                     /site_type="mutagenized site"
                     /note="<enter text here>"
                     /label="Kozak sequence"
     CDS             13..2883
                     /cds_type=ORF
                     /note="Length: 2868"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     CDS             13..2811
                     /cds_type=ORF
                     /label="SARS CoV MA Nsp12"
     Site            2812..2877
                     /site_type="mutagenized site"
                     /label="3x FLAG tag"
     Site            2878..2883
                     /site_type="mutagenized site"
                     /label="double stop"
     Site            2884..2897
                     /site_type="mutagenized site"
                     /note="XhoI and NotI restriction sites"
                     /label="3' RE site"
ORIGIN
        1 gtcgacgcca ccATGTCTGC GGATGCATCA ACGTTTTTAA ACCGGGTTTG CGGTGTAAGT
       61 GCAGCCCGTC TTACACCGTG CGGCACAGGC ACTAGTACTG ATGTCGTCTA CAGGGCTTTT
      121 GATATTTACA ACGAAAAAGT TGCTGGTTTT GCAAAGTTCC TAAAAACTAA TTGCTGTCGC
      181 TTCCAGGAGA AGGATGAGGA AGGCAATTTA TTAGACTCTT ACTTTGTAGT TAAGAGGCAT
      241 ACTATGTCTA ACTACCAACA TGAAGAGACT ATTTATAACT TGGTTAAAGA TTGTCCAGCG
      301 GTTGCTGTCC ATGACTTTTT CAAGTTTAGA GTAGATGGTG ACATGGTACC ACATATATCA
      361 CGTCAGCGTC TAACTAAATA CACAATGGCT GATTTAGTCT ATGCTCTACG TCATTTTGAT
      421 GAGGGTAATT GTGATACATT AAAAGAAATA CTCGTCACAT ACAATTGCTG TGATGATGAT
      481 TATTTCAATA AGAAGGATTG GTATGACTTC GTAGAGAATC CTGACATCTT ACGCGTATAT
      541 GCTAACTTAG GTGAGCGTGT ACGCCAATCA TTATTAAAGA CTGTACAATT CTGCGATGCT
      601 ATGCGTGATG CAGGCATTGT AGGCGTACTG ACATTAGATA ATCAGGATCT TAATGGGAAC
      661 TGGTACGATT TCGGTGATTT CGTACAAGTA GCACCAGGCT GCGGAGTTCC TATTGTGGAT
      721 TCATATTACT CATTGCTGAT GCCCATCCTC ACTTTGACTA GGGCATTGGC TGCTGAGTCC
      781 CATATGGATG CTGATCTCGC AAAACCACTT ATTAAGTGGG ATTTGCTGAA ATATGATTTT
      841 ACGGAAGAGA GACTTTGTCT CTTCGACCGT TATTTTAAAT ATTGGGACCA GACATACCAT
      901 CCCAATTGTA TTAACTGTTT GGATGATAGG TGTATCCTTC ATTGTGCAAA CTTTAATGTG
      961 TTATTTTCTA CTGTGTTTCC ACCTACAAGT TTTGGACCAC TAGTAAGAAA AATATTTGTA
     1021 GATGGTGTTC CTTTTGTTGT TTCAACTGGA TACCATTTTC GTGAGTTAGG AGTCGTACAT
     1081 AATCAGGATG TAAACTTACA TAGCTCGCGT CTCAGTTTCA AGGAACTTTT AGTGTATGCT
     1141 GCTGATCCAG CTATGCATGC AGCTTCTGGC AATTTATTGC TAGATAAACG CACTACATGC
     1201 TTTTCAGTAG CTGCACTAAC AAACAATGTT GCTTTTCAAA CTGTCAAACC CGGTAATTTT
     1261 AATAAAGACT TTTATGACTT TGCTGTGTCT AAAGGTTTCT TTAAGGAAGG AAGTTCTGTT
     1321 GAACTAAAAC ACTTCTTCTT TGCTCAGGAT GGCAACGCTG CTATCAGTGA TTATGACTAT
     1381 TATCGTTATA ATCTGCCAAC AATGTGTGAT ATCAGACAAC TCCTATTCGT AGTTGAAGTT
     1441 GTTGATAAAT ACTTTGATTG TTACGATGGT GGCTGTATTA ATGCCAACCA AGTAATCGTT
     1501 AACAATCTGG ATAAATCAGC TGGTTTCCCA TTTAATAAAT GGGGTAAGGC TAGACTTTAT
     1561 TATGACTCAA TGAGTTATGA GGATCAAGAT GCACTTTTCG CGTATACTAA GCGTAATGTC
     1621 ATCCCTACTA TAACTCAAAT GAATCTTAAG TATGCCATTA GTGCAAAGAA TAGAGCTCGC
     1681 ACCGTAGCTG GTGTCTCTAT CTGTAGTACT ATGACAAATA GACAGTTTCA TCAGAAATTA
     1741 TTGAAGTCAA TAGCCGCCAC TAGAGGAGCT ACTGTGGTAA TTGGAACAAG CAAGTTTTAC
     1801 GGTGGCTGGC ATAATATGTT AAAAACTGTT TACAGTGATG TAGAAACTCC ACACCTTATG
     1861 GGTTGGGATT ATCCAAAATG TGACAGAGCC ATGCCTAACA TGCTTAGGAT AATGGCCTCT
     1921 CTTGTTCTTG CTCGCAAACA TAACACTTGC TGTAACTTAT CACACCGTTT CTACAGGTTA
     1981 GCTAACGAGT GTGCGCAAGT ATTAAGTGAG ATGGTCATGT GTGGCGGCTC ACTATATGTT
     2041 AAACCAGGTG GAACATCATC CGGTGATGCT ACAACTGCTT ATGCTAATAG TGTCTTTAAC
     2101 ATTTGTCAAG CTGTTACAGC CAATGTAAAT GCACTTCTTT CAACTGATGG TAATAAGATA
     2161 GCTGACAAGT ATGTCCGCAA TCTACAACAC AGGCTCTATG AGTGTCTCTA TAGAAATAGG
     2221 GATGTTGATC ATGAATTCGT GGATGAGTTT TACGCTTACC TGCGTAAACA TTTCTCCATG
     2281 ATGATTCTTT CTGATGATGC CGTTGTGTGC TATAACAGTA ACTATGCGGC TCAAGGTTTA
     2341 GTAGCTAGCA TTAAGAACTT TAAGGCAGTT CTTTATTATC AAAATAATGT GTTCATGTCT
     2401 GAGGCAAAAT GTTGGACTGA GACTGACCTT ACTAAAGGAC CTCACGAATT TTGCTCACAG
     2461 CATACAATGC TAGTTAAACA AGGAGATGAT TACGTGTACC TGCCTTACCC AGATCCATCA
     2521 AGAATATTAG GCGCAGGCTG TTTTGTCGAT GATATTGTCA AAACAGATGG TACACTTATG
     2581 ATTGAAAGGT TCGTGTCACT GGCTATTGAT GCTTACCCAC TTACAAAACA TCCTAATCAG
     2641 GAGTATGCTG ATGTCTTTCA CTTGTATTTA CAATACATTA GAAAGTTACA TGATGAGCTT
     2701 ACTGGCCACA TGTTGGACAT GTATTCCGTA ATGCTAACTA ATGATAACAC CTCACGGTAC
     2761 TGGGAACCTG AGTTTTATGA GGCTATGTAC ACACCACATA CAGTCTTGCA Ggactacaaa
     2821 gaccatgacg gtgattataa agatcatgat atcgattaca aggatgacga tgacaagtag
     2881 taactcgagg cggccgc
//