LOCUS       pOME0262      1910 bp    DNA     linear   UNA 19-Aug-2015
DEFINITION  
FEATURES             Location/Qualifiers
     Site            1..12
                     /site_type="mutagenized site"
                     /note="SalI/KpnI restriction sites"
                     /label="5' Restriction sites"
     Site            13..18
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             19..1824
                     /cds_type=ORF
                     /label="SARS CoV MA Nsp13"
     CDS             19..1896
                     /cds_type=ORF
     Site            1825..1890
                     /site_type="mutagenized site"
                     /label="3X FLAG tag"
     Site            1891..1896
                     /site_type="mutagenized site"
                     /label="double stop"
     Site            1897..1910
                     /site_type="mutagenized site"
                     /note="XhoI and NotI restriction sites"
                     /label="3' Restriction sites"
ORIGIN
        1 gtcgacggta ccgccaccAT GGCTGTAGGT GtTTGTGTAT TGTGCAATTC ACAGACTTCA
       61 CTTCGTTGCG GTGCCTGTAT TAGGAGACCA TTCCTATGTT GCAAGTGCTG CTATGACCAT
      121 GTCATTTCAA CATCACACAA ATTAGTGTTG TCTGTTAATC CCTATGTTTG CAATGCCCCA
      181 GGTTGTGATG TCACTGATGT GACACAACTG TATCTAGGAG GTATGAGCTA TTATTGCAAG
      241 TCACATAAGC CTCCCATTAG TTTTCCATTA TGTGCTAATG GTCAGGTTTT TGGTTTATAC
      301 AAAAACACAT GTGTAGGCAG TGACAATGTC ACTGACTTCA ATGCGATAGC AACATGTGAT
      361 TGGACTAATG CTGGCGATTA CATACTTGCC AACACTTGTA CTGAGAGACT CAAGCTTTTC
      421 GCAGCAGAAA CGCTCAAAGC CACTGAGGAA ACATTTAAGC TGTCATATGG TATTGCTACT
      481 GTACGCGAAG TACTCTCTGA CAGAGAATTG CATCTTTCAT GGGAGGTTGG AAAACCTAGA
      541 CCACCATTGA ACAGAAACTA TGTCTTTACT GGTTACCGTG TAACTAAAAA TAGTAAAGTA
      601 CAGATTGGAG AGTACACCTT TGAAAAAGGT GACTATGGTG ATGCTGTTGT GTACAGAGGT
      661 ACTACGACAT ACAAGTTGAA TGTTGGTGAT TACTTTGTGT TGACATCTCA CACTGTAATG
      721 CCACTTAGTG CACCTACTCT AGTGCCACAA GAGCACTATG TGAGAATTAC TGGCTTGTAC
      781 CCAACACTCA ACATCTCAGA TGAGTTTTCT AGCAATGTTG CAAATTATCA AAAGGTCGGC
      841 ATGCAAAAGT ACTCTACACT CCAAGGACCA CCTGGTACTG GTAAGAGTCA TTTTGCCATC
      901 GGACTTGCTC TCTATTACCC ATCTGCTCGC ATAGTGTATA CGGCATGCTC TCATGCAGCT
      961 GTTGATGCCC TATGTGAAAA GGCATTAAAA TATTTGCCCA TAGATAAATG TAGTAGAATC
     1021 ATACCTGCGC GTGCGCGCGT AGAGTGTTTT GATAAATTCA AAGTGAATTC AACACTAGAA
     1081 CAGTATGTTT TCTGCACTGT AAATGCATTG CCAGAAACAA CTGCTGACAT TGTAGTCTTT
     1141 GATGAAATCT CTATGGCTAC TAATTATGAC TTGAGTGTTG TCAATGCTAG ACTTCGTGCA
     1201 AAACACTACG TCTATATTGG CGATCCTGCT CAATTACCAG CCCCCCGCAC ATTGCTGACT
     1261 AAAGGCACAC TAGAACCAGA ATATTTTAAT TCAGTGTGCA GACTTATGAA AACAATAGGT
     1321 CCAGACATGT TCCTTGGAAC TTGTCGCCGT TGTCCTGCTG AAATTGTTGA CACTGTGAGT
     1381 GCTTTAGTTT ATGACAATAA GCTAAAAGCA CACAAGGATA AGTCAGCTCA ATGCTTCAAA
     1441 ATGTTCTACA AAGGTGTTAT TACACATGAT GTTTCATCTG CAATCAACAG ACCTCAAATA
     1501 GGCGTTGTAA GAGAATTTCT TACACGCAAT CCTGCTTGGA GAAAAGCTGT TTTTATCTCA
     1561 CCTTATAATT CACAGAACGC TGTAGCTTCA AAAATCTTAG GATTGCCTAC GCAGACTGTT
     1621 GATTCATCAC AGGGTTCTGA ATATGACTAT GTCATATTCA CACAAACTAC TGAAACAGCA
     1681 CACTCTTGTA ATGTCAACCG CTTCAATGTG GCTATCACAA GGGCAAAAAT TGGCATTTTG
     1741 TGCATAATGT CTGATAGAGA TCTTTATGAC AAACTGCAAT TTACAAGTCT AGAAATACCA
     1801 CGTCGCAATG TGGCTACATT ACAAgactac aaagaccatg acggtgatta taaagatcat
     1861 gatatcgatt acaaggatga cgatgacaag tagtaactcg aggcggccgc
//