LOCUS       pOME0264      1688 bp    DNA     linear   UNA 19-Aug-2015
DEFINITION  
FEATURES             Location/Qualifiers
     Site            1..12
                     /site_type="mutagenized site"
                     /note="SalI/KpnI restriction sites"
                     /label="5' RE site"
     Site            13..18
                     /site_type="mutagenized site"
                     /label="Kozak site"
     CDS             19..1674
                     /cds_type=ORF
                     /note="Length: 1587"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     CDS             19..1602
                     /cds_type=ORF
                     /label="SARS CoV MA Nsp14"
     Site            1603..1668
                     /site_type="mutagenized site"
                     /label="3X FLAG tag"
     Site            1669..1674
                     /site_type="mutagenized site"
                     /label="double stop"
     Site            1675..1688
                     /site_type="mutagenized site"
                     /note="XhoI/NotI restriction sites"
                     /label="3' RE site"
ORIGIN
        1 gtcgacggta ccgccaccAT GGCAGAAAAT GTAACTGGAC TTTTTAAGGA CTGTAGTAAG
       61 ATCATTACTG GTCTTCATCC TACACAGGCA CCTACACACC TCAGCGTTGA TATAAAGTTC
      121 AAGACTGAAG GATTATGTGT TGACATACCA GGCATACCAA AGGACATGAC CTACCGTAGA
      181 CTCATCTCTA TGATGGGTTT CAAAATGAAT TACCAAGTCA ATGGTTACCC TAATATGTTT
      241 ATCACCCGCG AAGAAGCTAT TCGTCACGTT CGTGCGTGGA TTGGCTTTGA TGTAGAGGGC
      301 TGTCATGCAA CTAGAGATGC TGTGGGTACT AACCTACCTC TCCAGCTAGG ATTTTCTACA
      361 GGTGTTAACT TAGTAGCTGT ACCGACTGGT TATGTTGACA CTGAAAATAA CACAGAATTC
      421 ACCAGAGTTA ATGCAAAACC TCCACCAGGT GACCAGTTTA AACATCTTAT ACCACTCATG
      481 TATAAAGGCT TGCCCTGGAA TGTAGTGCGT ATTAAGATAG TACAAATGCT CAGTGATACA
      541 CTGAAAGGAT TGTCAGACAG AGTCGTGTTC GTCCTTTGGG CGCATGGCTT TGAGCTTACA
      601 TCAATGAAGT ACTTTGTCAA GATTGGACCT GAAAGAACGT GTTGTCTGTG TGACAAACGT
      661 GCAACTTGCT TTTCTACTTC ATCAGATACT TATGCCTGCT GGAATCATTC TGTGGGTTTT
      721 GACTATGTCT ATAACCCATT TATGATTGAT GTTCAGCAGT GGGGCTTTAC GGGTAACCTT
      781 CAGAGTAACC ATGACCAACA TTGCCAGGTA CATGGAAATG CACATGTGGC TAGTTGTGAT
      841 GCTATCATGA CTAGATGTTT AGCAGTCCAT GAGTGCTTTG TTAAGCGCGT TGATTGGTCT
      901 GTTGAATACC CTATTATAGG AGATGAACTG AGGGTTAATT CTGCTTGCAG AAAAGTACAA
      961 CACATGGTTG TGAAGTCTGC CCTCCTGGCT GATAAGTTTC CAGTTCTTCA TGACATTGGA
     1021 AATCCAAAGG CTATCAAGTG TGTGCCTCAG GCTGAAGTAG AATGGAAGTT CTACGATGCT
     1081 CAGCCATGTA GTGACAAAGC TTACAAAATA GAGGAGCTCT TCTATTCTTA TGCTACACAT
     1141 CACGATAAAT TCACTGATGG TGTTTGTTTG TTTTGGAATT GTAACGTTGA TCGTTACCCA
     1201 GCCAATGCAA TTGTGTGTAG GTTTGACACA AGAGTCTTGT CAAACTTGAA CTTACCAGGC
     1261 TGTGATGGTG GTAGTTTGTA TGTGAATAAG CATGCATTCC ACACTCCAGC TTTCGATAAA
     1321 AGTGCATTTA CTAATTTAAA GCAATTGCCT TTCTTTTACT ATTCTGATAG TCCTTGTGAG
     1381 TCTCATGGCA AACAAGTAGT GTCGGATATT GATTATGTTC CACTCAAATC TGCTACGTGT
     1441 ATTACACGAT GCAATTTAGG TGGTGCTGTT TGCAGACACC ATGCAAATGA GTACCGACAG
     1501 TACTTGGATG CATATAATAT GATGATTTCT GCTGGATTTA GCCTATGGAT TTACAAACAA
     1561 TTTGATACTT ATAACCTGTG GAATACATTT ACCAGGTTAC AGgactacaa agaccatgac
     1621 ggtgattata aagatcatga tatcgattac aaggatgacg atgacaagta gtaactcgag
     1681 gcggccgc
//