LOCUS       pOME0268       326 bp    DNA     linear   UNA 19-Aug-2015
DEFINITION  SARS CoV MA Envelope TAGGED
FEATURES             Location/Qualifiers
     Site            1..6
                     /site_type="restriction site"
                     /label=KpnI
     Site            1..6
                     /site_type="mutagenized site"
                     /note="KpnI restriction sites"
                     /label="5' RE sites"
     Site            7..12
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             13..240
                     /label="envelope protein"
     Site            241..306
                     /site_type="mutagenized site"
                     /label="3X FLAG tag"
     Site            307..312
                     /site_type="mutagenized site"
                     /label="double stop"
     Site            313..318
                     /site_type="restriction site"
                     /label=XhoI
     Site            313..326
                     /site_type="mutagenized site"
                     /note="XhoI/NotI restriction sites"
                     /label="3' restriction enzyme sites"
     Site            319..326
                     /site_type="restriction site"
                     /label=NotI
ORIGIN
        1 ggtaccgcca ccATGTACTC ATTCGTTTCG GAAGAAACAG GTACGTTAAT AGTTAATAGC
       61 GTACTTCTTT TTCTTGCTTT CGTGGTATTC TTGCTAGTCA CACTAGCCAT CCTTACTGCG
      121 CTTCGATTGT GTGCGTACTG CTGCAATATT GTTAACGTGA GTTTAGTAAA ACCAACGGTT
      181 TACGTCTACT CGCGTGTTAA AAATCTGAAC TCTTCTGAAG GAGTTCCTGA TCTTCTGGTC
      241 gactacaaag accatgacgg tgattataaa gatcatgata tcgattacaa ggatgacgat
      301 gacaagtagt aactcgaggc ggccgc
//