LOCUS pOME0268 326 bp DNA linear UNA 19-Aug-2015 DEFINITION SARS CoV MA Envelope TAGGED FEATURES Location/Qualifiers Site 1..6 /site_type="restriction site" /label=KpnI Site 1..6 /site_type="mutagenized site" /note="KpnI restriction sites" /label="5' RE sites" Site 7..12 /site_type="mutagenized site" /label="Kozak sequence" CDS 13..240 /label="envelope protein" Site 241..306 /site_type="mutagenized site" /label="3X FLAG tag" Site 307..312 /site_type="mutagenized site" /label="double stop" Site 313..318 /site_type="restriction site" /label=XhoI Site 313..326 /site_type="mutagenized site" /note="XhoI/NotI restriction sites" /label="3' restriction enzyme sites" Site 319..326 /site_type="restriction site" /label=NotI ORIGIN 1 ggtaccgcca ccATGTACTC ATTCGTTTCG GAAGAAACAG GTACGTTAAT AGTTAATAGC 61 GTACTTCTTT TTCTTGCTTT CGTGGTATTC TTGCTAGTCA CACTAGCCAT CCTTACTGCG 121 CTTCGATTGT GTGCGTACTG CTGCAATATT GTTAACGTGA GTTTAGTAAA ACCAACGGTT 181 TACGTCTACT CGCGTGTTAA AAATCTGAAC TCTTCTGAAG GAGTTCCTGA TCTTCTGGTC 241 gactacaaag accatgacgg tgattataaa gatcatgata tcgattacaa ggatgacgat 301 gacaagtagt aactcgaggc ggccgc //