LOCUS       pOME0276M       711 bp    DNA     linear   UNA 14-OCT-2015
DEFINITION  Reversed sequence of Human herpesvirus 8 strain JSC-1 clone BAC16, complete genome. TO_7 Control for pOME0275
ACCESSION   GQ994935
VERSION     GQ994935.1  GI:261853473
KEYWORDS    .
SOURCE      Human herpesvirus 8 Viruses; dsDNA viruses, no RNA stage; (Kaposi's sarcoma-associated herpesvirus)
  ORGANISM  Human herpesvirus 8 Viruses; dsDNA viruses, no RNA stage;
            Herpesvirales; Herpesviridae; Gammaherpesvirinae; Rhadinovirus.
REFERENCE   1  (bases 1 to 138146)
  AUTHORS   Brulois,K.F., Chang,H., Lee,A.S., Ensser,A., Wong,L.Y., Toth,Z.,
            Lee,S.H., Lee,H.R., Myoung,J., Ganem,D., Oh,T.K., Kim,J.F.,
            Gao,S.J. and Jung,J.U.
  TITLE     Construction and manipulation of a new Kaposi's sarcoma-associated
            herpesvirus Bacterial artificial chromosome clone
  JOURNAL   J. Virol. 86 (18), 9708-9720 (2012)
   PUBMED   22740391
REFERENCE   2  (bases 1 to 138146)
  AUTHORS   Brulois,K., Lee,A. and Chang,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2009) Molecular Microbiology and Immunology,
            University of Southern California, 1450 Biggy Street, Los Angeles,
            CA 90033, USA
COMMENT     
FEATURES             Location/Qualifiers
     misc_RNA        complement(1..711)
                     /note="coordinates estimated by looking at Fig, 2"
                     /label="as-ORF65-69 lncRNA - Ganem lab 2010"
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=#ffff80
     CDS             complement(1..711)
                     /label="as-ORF65-69 lncRNA"
                     /ApEinfo_fwdcolor=#0080ff
                     /ApEinfo_revcolor=#80ffff
     mRNA            1..711
                     /note=source?
                     /label=T0.7
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /label="Reverse compliment of T0.7"
     Site            1..7
                     /site_type="mutagenized site"
                     /label="Restriction site"
     polyA_site      10
                     /note="corresp. pos."
                     /label="TTS K12 - Wilson lab 2005, Gao lab 2014"
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
     polyA_signal    20..25
                     /note="corresp. pos."
                     /label="PAS K12 - Gao lab 2014"
                     /ApEinfo_fwdcolor=#ff0080
                     /ApEinfo_revcolor=pink
     misc_RNA        270..291
                     /label="miR-K12-3p -  miRbase.org"
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=#ffff80
     misc_RNA        307..329
                     /label="miR-K12-5p -  miRbase.org"
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=#ffff80
     CDS             497..679
                     /codon_start=1
                     /product="K12"
                     /protein_id="ACY00474.1"
                     /db_xref="GI:261853552"
                     /translation="MDRGLTVFVAVHVPDVLLNGWRWRLGAIPPLVCLLAISVVPPSG
                     QRGPVAFRTRVATGAH"
                     /label="K12 (kaposin)"
                     /ApEinfo_fwdcolor=#0080ff
                     /ApEinfo_revcolor=#80ffff
                     /label="Reverse Compliment of K12 (kaposin)"
     misc_RNA        547..569
                     /note="corresp. pos."
                     /label=miR-K10
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=#ffff80
     repeat_region   complement(701..711)
                     /note="26 copies"
                     /rpt_family="DR5"
                     /rpt_type=direct
                     /label="as-ORF65-69 lncRNA - Ganem lab 2010(1)"
                     /ApEinfo_label="as-ORF65-69 lncRNA - Ganem lab 2010"
                     /ApEinfo_fwdcolor=#00ffff
                     /ApEinfo_revcolor=pink
     Site            706..711
                     /site_type="mutagenized site"
                     /label="Restriction site"
ORIGIN
        1 GAATTCTTCT TTTAATCTGT TTATTGTTTT TGAACATGTG GCACACGCTG CAATGTACTG
       61 CCATGAAAGG TGGTTCTATA TCCACCACTT GGCGTCTGCC GAAGTCAGTG CCACAATTTC
      121 ATTAACAAAC AAGGTCAATA CATTCTGAGG GAGTGTTTTT TGCCATGGTA CCATTCGTGT
      181 GGTTTGGGAG AGCGGACGCC ATTTGCGTGC AAAATGTGCT TTGCTGGAGG CCAACTTCCG
      241 TCGCGCTGGT TGATGCGCGG CACATTGTGT CAACCAGGGC ACCCTCCCCC ACCGAGTGCT
      301 TTAATGCGGA GAGGAATGGT GGCCTGGTTG ACACCGCGTG CCGGCCTTCT GAACTGTGAC
      361 TGTGTTATGA GCCACGGGTA TGCCCTCGAT ACGCCTGCTC TTCAGCATTG TATGTGTTTA
      421 ATGTTGTGCT TGGTGCAACC GTGATTGTGC TTTTGTATTT TATTTTACTG ACACTCTTTG
      481 GGAGGGCACG CTAGCTTCAG TGCGCGCCCG TTGCAACTCG TGTCCTGAAT GCTACGGGGC
      541 CACGCTGGCC ACTCGGGGGG ACAACACTAA TCGCCAACAG ACAAACGAGT GGTGGTATCG
      601 CCCCAAGCCT CCAGCGCCAC CCATTTAGTA ACACATCCGG GACATGAACT GCCACAAACA
      661 CCGTTAAGCC TCTATCCATG CATTGGGATT GGAGTGAGGA GGGAGGGATC C
//