LOCUS       pOME0280       737 bp    DNA     linear   UNA 05-Feb-2016
DEFINITION  
FEATURES             Location/Qualifiers
     Site            1..6
                     /site_type="restriction site"
                     /label=SalI
     Site            2..12
                     /site_type="mutagenized site"
                     /label="Restriction sites"
     Site            7..12
                     /site_type="restriction site"
                     /label=KpnI
     Site            13..18
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             19..>737
                     /cds_type=ORF
                     /note="Length: 636"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     Site            652..717
                     /site_type="mutagenized site"
                     /label="3X FLAG"
     Site            718..723
                     /site_type="mutagenized site"
                     /label="double stop"
     Site            724..729
                     /site_type="restriction site"
                     /label=XhoI
     Site            730..737
                     /site_type="restriction site"
                     /label=NotI
ORIGIN
        1 gtcgacggta ccgccaccAT GCTTGCTTCA TACACTATCA CCCATTTCGG CAAGGCAACC
       61 AATCCACTTG TCAGAGTCAA TCGGCTGGGT CCTGGAATCC CGGATCATCC CCTCAGGCTC
      121 CTGCGAATTG GAAACCAGGC TTTCCTCCAG GAGTTCGTTC TTCCGCCAGT CCAACTACCC
      181 CAGTATTTCA CCTTTGATTT GACAGCACTC AAACTGATCA CCCAACCACT GCCTGCTGCA
      241 ACATGGACCG ATGACACTCC AACAGGATCA AATGGAGCGT TGCGTCCAGG AATTTCATTT
      301 CATCCAAAAC TTCGCCCCAT TCTTTTACCC AACAAAAGTG GGAAGAAGGG GAACAGTGCC
      361 GATCTAACAT CTCCGGAGAA AATCCAAGCA ATAATGACTT CACTCCAGGA CTTTAAGATC
      421 GTTCCAATTG ATCCAACCAA AAATATCATG GGAATCGAAG TGCCAGAAAC TCTGGTCCAC
      481 AAGCTGACCG GTAAGAAGGT GACTTCTAAA AATGGACAAC CAATCATCCC TGTTCTTTTG
      541 CCAAAGTACA TTGGGTTGGA CCCGGTGGCT CCAGGAGACC TCACCATGGT AATCACACAG
      601 GATTGTGACA CGTGTCATTC TCCTGCAAGT CTTCCAGCTG TGATTGAGAA Ggactacaaa
      661 gaccatgacg gtgattataa agatcatgat atcgattaca aggatgacga tgacaagtag
      721 taactcgagg cggccgc
//