LOCUS pOME0286 1430 bp DNA linear UNA 15-Feb-2016 DEFINITION FEATURES Location/Qualifiers Site 1..6 /site_type="mutagenized site" /label="5' RE" Site 7..12 /site_type="mutagenized site" /label="Kozak seq" CDS 13..>1416 /cds_type=ORF /note="Length: 1335" /note="Found at strand: positive" /note="Start codon: atg" CDS 13..1344 /cds_type=ORF /label="KSHV ORF36" Site 1345..1410 /site_type="mutagenized site" /label="3X FLAG" Site 1411..1416 /site_type="mutagenized site" /label="double STOP" Site 1417..1430 /site_type="mutagenized site" /label="3' RE" ORIGIN 1 gtcgacgcca ccatgcgctg gaagagaatg gagaggagac ccccactcac tcctcttcgg 61 agatcaagga cacaatcgtc aggtggaggc ttgacgattt gcccccggtg tgccctgaaa 121 ctccctaagg ctacccggat ttcagagaga ccctgggcgt ccacatggca gctgaatcag 181 catatacagg tgtccaagac taaaaaggcc accgcgtatc ttaaagcgcc ccgtgaatgg 241 gggcagtgca cgcaccagga tccagactgg tccaagcgtc tgggtcgtgg cgcctttggc 301 ataatcgtcc ctatctccga ggatctgtgt gtgaagcagt ttgatagccg ccgggagttt 361 ttctacgagg caattgccaa cgacctgatg caggccaccc gagagaggta ccccatgcat 421 tctggtggat ctagactgct aggattcgtg cagccttgca taccctgtag atcgattgtg 481 tatcctagaa tgaagtgcaa cctgctgcag ctggactgga gtcaggtcaa cctgagtgtc 541 atggcggcgg agttcaccgg cctaatggcg gcggtgtcct ttctaaacag atactgtggc 601 atggtgcact gcgacgttag tccagacaat attttggcca caggagacct aacgcccatg 661 aaccccggga ggctggtcct taccgatttc ggttccgttg cgctacactc tgggagcaag 721 tggactaacc ttgtggtgac ctctaacctg gggtttaagc aacactgcta cgacttcagg 781 gtgccaccca aactcatttg taagcatctc tataagccgt cttgcgtcct cttccagtgt 841 tacctatcca gtctcggtaa gatgcacgcg caggtattgg accaaccgta ccctatcagc 901 cctaacatgg gactgaccat cgacatgtcc tcgttgggct acactctgct gacatgcctg 961 gaactctatc tcgatctgcc gctaaacaac cctctgaagt tcttgggttc agccaccaga 1021 gacggacgcc ccgaacccat gtactacttg ggcttcatga ttcccagggt ggtgatgact 1081 cagatcctgt ccgctgtgtg gaccatgacg cttgacctgg gactagattg caccggcaaa 1141 gcccaggcga ttcccatgcg acaggagcac cagctggcgt ttcagaagca gtgctattta 1201 tataaagcca accaaaaggc agagtcgtta gcgaactgct ccgataagct aaactgcccc 1261 atgttaaagt ctctcgttag aaagctacta gagcgagact ttttcaacca tggaggccac 1321 ccccacaccc gcggacttgt tttcgactac aaagaccatg acggtgatta taaagatcat 1381 gatatcgatt acaaggatga cgatgacaag tagtaactcg aggcggccgc //