LOCUS       pOME0292M      1093 bp    DNA     linear   VRL 27-AUG-2012
DEFINITION  PAN-RNA_RevHuman herpesvirus 8 strain JSC-1 clone BAC16, complete genome.
ACCESSION   GQ994935
VERSION     GQ994935.1  GI:261853473
KEYWORDS    .
SOURCE      Human herpesvirus 8 (Kaposi's sarcoma-associated herpesvirus)
  ORGANISM  Human herpesvirus 8
            Viruses; dsDNA viruses, no RNA stage; Herpesvirales; Herpesviridae;
            Gammaherpesvirinae; Rhadinovirus.
REFERENCE   1  (bases 1 to 138146)
  AUTHORS   Brulois,K.F., Chang,H., Lee,A.S., Ensser,A., Wong,L.Y., Toth,Z.,
            Lee,S.H., Lee,H.R., Myoung,J., Ganem,D., Oh,T.K., Kim,J.F.,
            Gao,S.J. and Jung,J.U.
  TITLE     Construction and manipulation of a new Kaposi's sarcoma-associated
            herpesvirus Bacterial artificial chromosome clone
  JOURNAL   J. Virol. 86 (18), 9708-9720 (2012)
   PUBMED   22740391
REFERENCE   2  (bases 1 to 138146)
  AUTHORS   Brulois,K., Lee,A. and Chang,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2009) Molecular Microbiology and Immunology,
            University of Southern California, 1450 Biggy Street, Los Angeles,
            CA 90033, USA
COMMENT     Reverse of pOME0244 to serve as negative control
FEATURES             Location/Qualifiers
     misc_RNA        <1..1081
                     /note="corresp. pos."
                     /label="PAN RNA - NC_009333 genome annotation"
     misc_RNA        1..1087
                     /note="corresp. pos."
                     /label="PAN RNA - Zheng lab 2013"
     source          <1..>1087
                     /organism="Human herpesvirus 8"
                     /mol_type="genomic DNA"
                     /strain="JSC-1"
                     /db_xref="taxon:37296"
                     /clone="BAC16"
                     /note="infectious BAC clone harboring full-length KSHV
                     genome; derived from rKSHV.219, KSHV-infected JSC1
                     primary effusion lymphoma cell line"
     Site            1..6
                     /site_type="mutagenized site"
                     /label="5' RE site"
     misc_RNA        8..1082
                     /note="from 2014 Arias et al. (Ganem lab) KSHV 2.0"
                     /label="PAN RNA - Ganem lab 2014"
     misc_RNA        8..>1087
                     /note="5' end not determine, est. based on Fig. 2"
                     /label="K4.5 (T6.1, as-K5-K6) - Ganem lab 2014"
     misc_RNA        8..>1087
                     /note="corresp. to 5' end ID'd by 2005 Taylor et al.
                     (Chang/Moore lab)"
                     /note="corresp. to PAN RNA 3' end ID'd by KSHV 2.0"
                     /note="corresp. pos."
                     /label="K4.5 (T6.1) - Chang/Moore lab 2005/2011, Ganemlab
                     2014"
     polyA_site      9
                     /note="corresp. pos."
                     /label="TTS K4.5(T6.1)-PAN-K7 - Zheng lab 2013"
     polyA_signal    24..29
                     /note="corresp. pos."
                     /label="PAS K4.5-PAN-K7 - Gao lab 2014"
     misc_RNA        complement(25..>1087)
                     /note="5' end approx."
                     /note="corresp. pos."
                     /label="K7.3 (anti-PAN lncRNA) - Chang/Moore lab 2011"
     polyA_site      471
                     /note="corresp. pos."
                     /label="TTS K4.5(T6.1)-PAN-K7 - Zheng lab 2013"
     CDS             746..>1087
                     /codon_start=1
                     /product="K7"
                     /protein_id="ACY00412.1"
                     /db_xref="GI:261853490"
                     /translation="MGTLEIKGASLSQFSTGTAQSPWLPLHLWILCSLLAFLPLLVFI
                     GAADCGLIASLLAIYPSWLSARFSVLLFPHWPESCSTKNTARSGALHKPAEQKLRFAQ
                     KPCHGNYTVTPCGLLHWIQSPGQL"
     polyA_site      823
                     /note="corresp. pos."
                     /label="TTS K4.5(T6.1)-PAN - Zheng lab 2013"
     Site            join(1088..1093,1088^1089)
                     /site_type="restriction site"
                     /note="Name: BamHI"
                     /note="Pattern: ggatcc"
                     /note="cut_0_on_positive_strand: 1088^1089"
                     /note="cut_0_on_negative_strand: 1092^1093"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=BamHI
ORIGIN
        1 GAATTCACCT AATTTGTAAC TGGAAATAAA TACAACATTC AACGTAATGC AATACCATAA
       61 TCAAATTAAA CTCGAGATCC GTGCAATTTA ACAGTTTTCA TATTGTACAA AAAGGTTATA
      121 TGTGACCCTA TTTTTGGAAC GGCAGACCAG TGACCTCCAC AGGCCACGCT TGTTCCTTTT
      181 TGGGTCGGTT TTGTGGCAAT AGTAACAATG TGTTGCGAAA GTGGATGTTC TTTTGTAGCA
      241 ATCAGTTGGA TCGTTTTGTA GTTTATAATC GGACCTTGAA GGTTGTGTTG TATGTGTAAT
      301 TTTGTAATTG TAACTTTCTC GCGAGGGTCG ACGGCGTGTG GTGAAATCAG GTTACAAGAA
      361 TGTGCTGAAA CTTTGAAGAC TGTTTACGGT GGAGTGAAAC AGCGGATACA GTAAGTTTAG
      421 CTGAACGAAT GTGACCTTTT TATTTGTGTG GTAATGTCGT GATCGGACTA TGTTAGATTT
      481 TGCGTAAAAT TTTACGAAGT GTTGCGTGGT TATTCTATGT GTAGGTCTAA CAGTGTAAAT
      541 CCCGTTTCAC CGGGCTAAAT GTGAGTTAGG CGAAAGATCT TAATGGAGTT GTGATAGATT
      601 CTTAGTCTGT TTGTGTCTTG GCTTTGTTGC TTACTCGTCT ATCCATCACG TGGTGACAAG
      661 ACTATGTGGT CACCCGCGAC GAAAGGAAAG TGTAATATAA GGTGTAAGTC TGTGCAATTC
      721 ATAGGAGCGT ATAGTATTTT CCCCCGATGT TGACCGGACC TCTAACTTAG GTTACGTTAT
      781 TGGGCGTTCC CCACTGACAT ATCAACGGTA CCGTTCCAAA AACCCGTTTA GCGTCGAAAA
      841 CAAGACGCCC GAATACCTCT CGAGGTCTGG CGCGCCACAA AAAACATGAT GTCGAGAGTC
      901 CGGTTACACC CTTTTCATCC TGCCTTTTGG ATCGGCTTTC GGTCCTACCC ATATAACGGT
      961 TTTCGCTGCG TTAGTTGGGT GTTAGCCGCC GTGGTTACTT TTGGTCTTCG CCGTTCTTCC
     1021 GTTCGTCGCT CGTGTTTTAG GTATCCACTT CGCCGTCGGT TCCACTGACC CGTCAGGGTC
     1081 ACGATTTGGA TCC
//