LOCUS pOME0293 1045 bp DNA linear VRL 27-AUG-2012 DEFINITION Reverse antiRTA1kb Human herpesvirus 8 strain JSC-1 clone BAC16, complete genome. ACCESSION GQ994935 VERSION GQ994935.1 GI:261853473 KEYWORDS . SOURCE Human herpesvirus 8 (Kaposi's sarcoma-associated herpesvirus) ORGANISM Human herpesvirus 8 Viruses; dsDNA viruses, no RNA stage; Herpesvirales; Herpesviridae; Gammaherpesvirinae; Rhadinovirus. REFERENCE 1 (bases 1 to 138146) AUTHORS Brulois,K.F., Chang,H., Lee,A.S., Ensser,A., Wong,L.Y., Toth,Z., Lee,S.H., Lee,H.R., Myoung,J., Ganem,D., Oh,T.K., Kim,J.F., Gao,S.J. and Jung,J.U. TITLE Construction and manipulation of a new Kaposi's sarcoma-associated herpesvirus Bacterial artificial chromosome clone JOURNAL J. Virol. 86 (18), 9708-9720 (2012) PUBMED 22740391 REFERENCE 2 (bases 1 to 138146) AUTHORS Brulois,K., Lee,A. and Chang,H. TITLE Direct Submission JOURNAL Submitted (21-SEP-2009) Molecular Microbiology and Immunology, University of Southern California, 1450 Biggy Street, Los Angeles, CA 90033, USA COMMENT Reverse for negative control FEATURES Location/Qualifiers misc_RNA <1..>1045 /note="corresp. pos." /label="K7.7 (T3.0 lncRNA) - Ganem lab 2010 (Xu et al.)" misc_RNA <1..1045 /note="corresp. pos." /label="K7.7 (T3.0 lncRNA) - Yuan lab 2002" misc_RNA 1..1045 /note="corresp. pos." /label="T1.2 lncRNA - Yuan lab 2002" misc_RNA <1..>1045 /note="corresp. pos." /label="T1.2 lncRNA - Ganem lab 2010 (Xu et al.)" CDS complement(<1..>1045) /codon_start=1 /product="ORF50" /protein_id="ACY00448.1" /db_xref="GI:261853526" /translation="MAQDDKGKKLRRSCVESFVGLSDELKAQLYQCVLLINDAYETIY DPSDLNRVVEDVCIRIMKECSKLGALCGLFTDINMFNLFCFFRASRMRTKGAAGYNVP CAEASQGIIRILTERILFCTEKAFLTAACSGVSLPPAICKLLHEIYTEMKAKCLGAWR RLVCNRRPIMILTSSLLKLYNTYDTAGLLSEQSRALCLLVFQPVYLPRIMAPLEIMTK GQLAPENFYSITGSAEKRRPITTGKVTGLSYPGSGLMPESLILPILEPGLLPASMVDL SDVLAKPAVILSAPALSQFVISKPHPNMPHTVSIIPFNPSGTDPAFISTWQAASQNMV YNTSTAPLKPATGSSQTVSVKAVAQGAVITATTVPQAMPARGTGGELPVMSASTPARD QVAACFVAENTGDSPDNPSSFLTSCHPCDPNTVIVAQQFQPPQCVTLLQVTCAPSSTP PPDSTVRAPVVQLPTVVPLPASAFLPALAQPEASGEELPGGHDGDQGVPCRDSTAAAT AAEATTPKRKQRSKERSSKKRKALTVPEADTTPSTTTPGTSLGSITTPQDVHATDVAT SEGPSEAQPPLLSLPPPLDVDQSLFALLDEAGPETWDVGSPLSPTDDALLSSILQGLY QLDTPPPLRSPSPASFGPESPADIPSPSGGEYTQLQPVRATSATPANEVQESGTLYQL HQWRNYFRD" /label="ORF50 (RTA)" source complement(<1..>1045) /organism="Human herpesvirus 8" /mol_type="genomic DNA" /strain="JSC-1" /db_xref="taxon:37296" /clone="BAC16" /note="infectious BAC clone harboring full-length KSHV genome; derived from rKSHV.219, KSHV-infected JSC1 primary effusion lymphoma cell line" Site 1..6 /site_type="mutagenized site" /label="5' RE site" polyA_signal 38..43 /label="PAS T1.2 lncRNA - Schifano guess" Site 1040..1045 /site_type="mutagenized site" /label="3' RE site" ORIGIN 1 GAATTCGGGG AAATTGGGTA GCCCATGTCT GGGCCGCAAA TAATCATGCA CCGTCCGGCG 61 CAGTGTCTTA TACCACATGT TGTGTAGGTG GCGCGGGAAT TTTGGCCGGT GGCCATCAAG 121 TGTCTGCCAC AGTCAGTTCC GCCAACGAGT TCCCCGGCAC TAATGACGCT GTTGCCACGG 181 CGTCCGTTAC GGTCGCGCCC CATGGCCTCC CCTCAACGGA CATTACAGTC GCAGGTGAGG 241 ACGTTCTCTA GTCCAGCGAC GTACAAAACA GCGTCTCTTG TGGCCTCTAA GAGGGCTGTT 301 GGGCTCGAGA AAGGACTGCA GTACAGTGGG AACGCTAGGC TTGTGCCAAT ATCACCGGGT 361 CGTTAAAGTT GGTGGCGTTA CGCAATGCAA CAACGTCCAA TGGACACGGG GGAGAAGCTG 421 TGGTGGGGGG CTAAGTTGTC AGGCCCGGGG CCACCACGTC AACGGTTGTC ATCAGGGAGA 481 CGGCCGGTCG CGCAAGGAGG GCCGCGAGCG GGTTGGTCTT CGGAGCCCGC TTCTCGAAGG 541 CCCGCCAGTA CTGCCTCTGG TTCCACACGG CACATCTCTA AGTTGCCGCC GCCGATGCCG 601 CCGTCTCCGC TGTTGTGGGT TTGCTTTCGT CTCTTCGTTT CTCTCCTCGA GTTTCTTCGC 661 ATTCCGAAAC TGGCACGGTC TTCGGCTGTG GTGCGGTAGC TGGTGCTGTG GACCATGGAG 721 AAACCCTAGT TAATGGTGGG GGGTCCTACA CGTGCGGTGC CTACAGCGGT GCAGACTCCC 781 TGGTAGCCTC CGTGTTGGGG GCGATGACAG CAATGGGGGC GGTGACCTGC ATCTAGTCTC 841 AGATAAGCGG GACAATCTGC TTCGCCCGGG ACTTTGTACC CTACAGCCCA GCGGAGAGAG 901 GGGGTGACTG CTGCGCGACA ACAGGTCATA AGACGTTCCT GACATGGTCG ACCTGTGCGG 961 TGGCGGAGAC GCCAGTGGGA GGGGGCGAAG GAAGCCGGGC CTCAGAGGCC GCCTATATGG 1021 CAGTGGAAGA CCACCTCTCG GATCC //