LOCUS       pOME0293      1045 bp    DNA     linear   VRL 27-AUG-2012
DEFINITION  Reverse antiRTA1kb Human herpesvirus 8 strain JSC-1 clone BAC16, complete genome.
ACCESSION   GQ994935
VERSION     GQ994935.1  GI:261853473
KEYWORDS    .
SOURCE      Human herpesvirus 8 (Kaposi's sarcoma-associated herpesvirus)
  ORGANISM  Human herpesvirus 8
            Viruses; dsDNA viruses, no RNA stage; Herpesvirales; Herpesviridae;
            Gammaherpesvirinae; Rhadinovirus.
REFERENCE   1  (bases 1 to 138146)
  AUTHORS   Brulois,K.F., Chang,H., Lee,A.S., Ensser,A., Wong,L.Y., Toth,Z.,
            Lee,S.H., Lee,H.R., Myoung,J., Ganem,D., Oh,T.K., Kim,J.F.,
            Gao,S.J. and Jung,J.U.
  TITLE     Construction and manipulation of a new Kaposi's sarcoma-associated
            herpesvirus Bacterial artificial chromosome clone
  JOURNAL   J. Virol. 86 (18), 9708-9720 (2012)
   PUBMED   22740391
REFERENCE   2  (bases 1 to 138146)
  AUTHORS   Brulois,K., Lee,A. and Chang,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2009) Molecular Microbiology and Immunology,
            University of Southern California, 1450 Biggy Street, Los Angeles,
            CA 90033, USA
COMMENT     Reverse for negative control
FEATURES             Location/Qualifiers
     misc_RNA        <1..>1045
                     /note="corresp. pos."
                     /label="K7.7 (T3.0 lncRNA) - Ganem lab 2010 (Xu et al.)"
     misc_RNA        <1..1045
                     /note="corresp. pos."
                     /label="K7.7 (T3.0 lncRNA) - Yuan lab 2002"
     misc_RNA        1..1045
                     /note="corresp. pos."
                     /label="T1.2 lncRNA - Yuan lab 2002"
     misc_RNA        <1..>1045
                     /note="corresp. pos."
                     /label="T1.2 lncRNA - Ganem lab 2010 (Xu et al.)"
     CDS             complement(<1..>1045)
                     /codon_start=1
                     /product="ORF50"
                     /protein_id="ACY00448.1"
                     /db_xref="GI:261853526"
                     /translation="MAQDDKGKKLRRSCVESFVGLSDELKAQLYQCVLLINDAYETIY
                     DPSDLNRVVEDVCIRIMKECSKLGALCGLFTDINMFNLFCFFRASRMRTKGAAGYNVP
                     CAEASQGIIRILTERILFCTEKAFLTAACSGVSLPPAICKLLHEIYTEMKAKCLGAWR
                     RLVCNRRPIMILTSSLLKLYNTYDTAGLLSEQSRALCLLVFQPVYLPRIMAPLEIMTK
                     GQLAPENFYSITGSAEKRRPITTGKVTGLSYPGSGLMPESLILPILEPGLLPASMVDL
                     SDVLAKPAVILSAPALSQFVISKPHPNMPHTVSIIPFNPSGTDPAFISTWQAASQNMV
                     YNTSTAPLKPATGSSQTVSVKAVAQGAVITATTVPQAMPARGTGGELPVMSASTPARD
                     QVAACFVAENTGDSPDNPSSFLTSCHPCDPNTVIVAQQFQPPQCVTLLQVTCAPSSTP
                     PPDSTVRAPVVQLPTVVPLPASAFLPALAQPEASGEELPGGHDGDQGVPCRDSTAAAT
                     AAEATTPKRKQRSKERSSKKRKALTVPEADTTPSTTTPGTSLGSITTPQDVHATDVAT
                     SEGPSEAQPPLLSLPPPLDVDQSLFALLDEAGPETWDVGSPLSPTDDALLSSILQGLY
                     QLDTPPPLRSPSPASFGPESPADIPSPSGGEYTQLQPVRATSATPANEVQESGTLYQL
                     HQWRNYFRD"
                     /label="ORF50 (RTA)"
     source          complement(<1..>1045)
                     /organism="Human herpesvirus 8"
                     /mol_type="genomic DNA"
                     /strain="JSC-1"
                     /db_xref="taxon:37296"
                     /clone="BAC16"
                     /note="infectious BAC clone harboring full-length KSHV
                     genome; derived from rKSHV.219, KSHV-infected JSC1
                     primary effusion lymphoma cell line"
     Site            1..6
                     /site_type="mutagenized site"
                     /label="5' RE site"
     polyA_signal    38..43
                     /label="PAS T1.2 lncRNA - Schifano guess"
     Site            1040..1045
                     /site_type="mutagenized site"
                     /label="3' RE site"
ORIGIN
        1 GAATTCGGGG AAATTGGGTA GCCCATGTCT GGGCCGCAAA TAATCATGCA CCGTCCGGCG
       61 CAGTGTCTTA TACCACATGT TGTGTAGGTG GCGCGGGAAT TTTGGCCGGT GGCCATCAAG
      121 TGTCTGCCAC AGTCAGTTCC GCCAACGAGT TCCCCGGCAC TAATGACGCT GTTGCCACGG
      181 CGTCCGTTAC GGTCGCGCCC CATGGCCTCC CCTCAACGGA CATTACAGTC GCAGGTGAGG
      241 ACGTTCTCTA GTCCAGCGAC GTACAAAACA GCGTCTCTTG TGGCCTCTAA GAGGGCTGTT
      301 GGGCTCGAGA AAGGACTGCA GTACAGTGGG AACGCTAGGC TTGTGCCAAT ATCACCGGGT
      361 CGTTAAAGTT GGTGGCGTTA CGCAATGCAA CAACGTCCAA TGGACACGGG GGAGAAGCTG
      421 TGGTGGGGGG CTAAGTTGTC AGGCCCGGGG CCACCACGTC AACGGTTGTC ATCAGGGAGA
      481 CGGCCGGTCG CGCAAGGAGG GCCGCGAGCG GGTTGGTCTT CGGAGCCCGC TTCTCGAAGG
      541 CCCGCCAGTA CTGCCTCTGG TTCCACACGG CACATCTCTA AGTTGCCGCC GCCGATGCCG
      601 CCGTCTCCGC TGTTGTGGGT TTGCTTTCGT CTCTTCGTTT CTCTCCTCGA GTTTCTTCGC
      661 ATTCCGAAAC TGGCACGGTC TTCGGCTGTG GTGCGGTAGC TGGTGCTGTG GACCATGGAG
      721 AAACCCTAGT TAATGGTGGG GGGTCCTACA CGTGCGGTGC CTACAGCGGT GCAGACTCCC
      781 TGGTAGCCTC CGTGTTGGGG GCGATGACAG CAATGGGGGC GGTGACCTGC ATCTAGTCTC
      841 AGATAAGCGG GACAATCTGC TTCGCCCGGG ACTTTGTACC CTACAGCCCA GCGGAGAGAG
      901 GGGGTGACTG CTGCGCGACA ACAGGTCATA AGACGTTCCT GACATGGTCG ACCTGTGCGG
      961 TGGCGGAGAC GCCAGTGGGA GGGGGCGAAG GAAGCCGGGC CTCAGAGGCC GCCTATATGG
     1021 CAGTGGAAGA CCACCTCTCG GATCC
//