LOCUS pOME0297 711 bp DNA linear UNA 14-OCT-2015 DEFINITION Human herpesvirus 8 strain JSC-1 clone BAC16, complete genome. ACCESSION GQ994935 VERSION GQ994935.1 GI:261853473 KEYWORDS . SOURCE Human herpesvirus 8 Viruses; dsDNA viruses, no RNA stage; (Kaposi's sarcoma-associated herpesvirus) ORGANISM Human herpesvirus 8 Viruses; dsDNA viruses, no RNA stage; Herpesvirales; Herpesviridae; Gammaherpesvirinae; Rhadinovirus. REFERENCE 1 (bases 1 to 138146) AUTHORS Brulois,K.F., Chang,H., Lee,A.S., Ensser,A., Wong,L.Y., Toth,Z., Lee,S.H., Lee,H.R., Myoung,J., Ganem,D., Oh,T.K., Kim,J.F., Gao,S.J. and Jung,J.U. TITLE Construction and manipulation of a new Kaposi's sarcoma-associated herpesvirus Bacterial artificial chromosome clone JOURNAL J. Virol. 86 (18), 9708-9720 (2012) PUBMED 22740391 REFERENCE 2 (bases 1 to 138146) AUTHORS Brulois,K., Lee,A. and Chang,H. TITLE Direct Submission JOURNAL Submitted (21-SEP-2009) Molecular Microbiology and Immunology, University of Southern California, 1450 Biggy Street, Los Angeles, CA 90033, USA COMMENT FEATURES Location/Qualifiers Site join(1..6,1^2) /site_type="restriction site" /note="Name: EcoRI" /note="Pattern: gaattc" /note="cut_0_on_positive_strand: 1^2" /note="cut_0_on_negative_strand: 5^6" /note="inhibited_by: 5': N6-methyladenosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label=EcoRI misc_RNA complement(7..705) /note="coordinates estimated by looking at Fig, 2" /label="as-ORF65-69 lncRNA - Ganem lab 2010" /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=#ffff80 CDS complement(7..705) /label="as-ORF65-69 lncRNA" /ApEinfo_fwdcolor=#0080ff /ApEinfo_revcolor=#80ffff mRNA 7..705 /note=source? /label=T0.7 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink polyA_site 10 /note="corresp. pos." /label="TTS K12 - Wilson lab 2005, Gao lab 2014" /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink polyA_signal 20..25 /note="corresp. pos." /label="PAS K12 - Gao lab 2014" /ApEinfo_fwdcolor=#ff0080 /ApEinfo_revcolor=pink misc_RNA 270..291 /label="miR-K12-3p - miRbase.org" /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=#ffff80 misc_RNA 307..329 /label="miR-K12-5p - miRbase.org" /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=#ffff80 CDS 497..679 /codon_start=1 /product="K12" /protein_id="ACY00474.1" /db_xref="GI:261853552" /translation="MDRGLTVFVAVHVPDVLLNGWRWRLGAIPPLVCLLAISVVPPSG QRGPVAFRTRVATGAH" /label="K12 (kaposin)" /ApEinfo_fwdcolor=#0080ff /ApEinfo_revcolor=#80ffff misc_RNA 547..569 /note="corresp. pos." /label=miR-K10 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=#ffff80 repeat_region complement(701..705) /note="26 copies" /rpt_family="DR5" /rpt_type=direct /label="as-ORF65-69 lncRNA - Ganem lab 2010(1)" /ApEinfo_label="as-ORF65-69 lncRNA - Ganem lab 2010" /ApEinfo_fwdcolor=#00ffff /ApEinfo_revcolor=pink Site join(706..711,706^707) /site_type="restriction site" /note="Name: BamHI" /note="Pattern: ggatcc" /note="cut_0_on_positive_strand: 706^707" /note="cut_0_on_negative_strand: 710^711" /note="inhibited_by: 5': N4-methylcytosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label=BamHI ORIGIN 1 GAATTCAAGA AAATTAGACA AATAACAAAA ACTTGTACAC CGTGTGCGAC GTTACATGAC 61 GGTACTTTCC ACCAAGATAT AGGTGGTGAA CCGCAGACGG CTTCAGTCAC GGTGTTAAAG 121 TAATTGTTTG TTCCAGTTAT GTAAGACTCC CTCACAAAAA ACGGTACCAT GGTAAGCACA 181 CCAAACCCTC TCGCCTGCGG TAAACGCACG TTTTACACGA AACGACCTCC GGTTGAAGGC 241 AGCGCGACCA ACTACGCGCC GTGTAACACA GTTGGTCCCG TGGGAGGGGG TGGCTCACGA 301 AATTACGCCT CTCCTTACCA CCGGACCAAC TGTGGCGCAC GGCCGGAAGA CTTGACACTG 361 ACACAATACT CGGTGCCCAT ACGGGAGCTA TGCGGACGAG AAGTCGTAAC ATACACAAAT 421 TACAACACGA ACCACGTTGG CACTAACACG AAAACATAAA ATAAAATGAC TGTGAGAAAC 481 CCTCCCGTGC GATCGAAGTC ACGCGCGGGC AACGTTGAGC ACAGGACTTA CGATGCCCCG 541 GTGCGACCGG TGAGCCCCCC TGTTGTGATT AGCGGTTGTC TGTTTGCTCA CCACCATAGC 601 GGGGTTCGGA GGTCGCGGTG GGTAAATCAT TGTGTAGGCC CTGTACTTGA CGGTGTTTGT 661 GGCAATTCGG AGATAGGTAC GTAACCCTAA CCTCACTCCT CCCTCGGATC C //