LOCUS       pOME0300M      1273 bp    DNA     linear   VRL 27-AUG-2012
DEFINITION  Reverse sequence of anti-PAN Human herpesvirus 8 strain JSC-1 clone BAC16, complete genome.
ACCESSION   GQ994935
VERSION     GQ994935.1  GI:261853473
KEYWORDS    .
SOURCE      Human herpesvirus 8 (Kaposi's sarcoma-associated herpesvirus)
  ORGANISM  Human herpesvirus 8
            Viruses; dsDNA viruses, no RNA stage; Herpesvirales; Herpesviridae;
            Gammaherpesvirinae; Rhadinovirus.
REFERENCE   1  (bases 1 to 138146)
  AUTHORS   Brulois,K.F., Chang,H., Lee,A.S., Ensser,A., Wong,L.Y., Toth,Z.,
            Lee,S.H., Lee,H.R., Myoung,J., Ganem,D., Oh,T.K., Kim,J.F.,
            Gao,S.J. and Jung,J.U.
  TITLE     Construction and manipulation of a new Kaposi's sarcoma-associated
            herpesvirus Bacterial artificial chromosome clone
  JOURNAL   J. Virol. 86 (18), 9708-9720 (2012)
   PUBMED   22740391
REFERENCE   2  (bases 1 to 138146)
  AUTHORS   Brulois,K., Lee,A. and Chang,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2009) Molecular Microbiology and Immunology,
            University of Southern California, 1450 Biggy Street, Los Angeles,
            CA 90033, USA
FEATURES             Location/Qualifiers
     polyA_signal    <1..11
                     /label="PAS K7.3(anti-PAN lncRNA) - Schifano guess"
     misc_RNA        complement(<1..>1273)
                     /note="5' end not determine, est. based on Fig. 2"
                     /label="K4.5 (T6.1, as-K5-K6) - Ganem lab 2014"
     misc_RNA        1..1273
                     /note="5' end approx."
                     /note="corresp. pos."
                     /label="K7.3 (anti-PAN lncRNA) - Chang/Moore lab 2011"
     misc_RNA        complement(<1..>1273)
                     /note="corresp. to 5' end ID'd by 2005 Taylor et al.
                     (Chang/Moore lab)"
                     /note="corresp. to PAN RNA 3' end ID'd by KSHV 2.0"
                     /note="corresp. pos."
                     /label="K4.5 (T6.1) - Chang/Moore lab 2005/2011,
                     Ganemlab2014"
     source          complement(<1..>1273)
                     /organism="Human herpesvirus 8"
                     /mol_type="genomic DNA"
                     /strain="JSC-1"
                     /db_xref="taxon:37296"
                     /clone="BAC16"
                     /note="infectious BAC clone harboring full-length KSHV
                     genome; derived from rKSHV.219, KSHV-infected JSC1
                     primary effusion lymphoma cell line"
     Site            1..6
                     /site_type="mutagenized site"
                     /label="5' RE site"
     CDS             complement(166..546)
                     /codon_start=1
                     /product="K7"
                     /protein_id="ACY00412.1"
                     /db_xref="GI:261853490"
                     /translation="MGTLEIKGASLSQFSTGTAQSPWLPLHLWILCSLLAFLPLLVFI
                     GAADCGLIASLLAIYPSWLSARFSVLLFPHWPESCSTKNTARSGALHKPAEQKLRFAQ
                     KPCHGNYTVTPCGLLHWIQSPGQL"
     misc_RNA        complement(205..>1273)
                     /note="corresp. pos."
                     /label="PAN RNA - Zheng lab 2013"
     misc_RNA        complement(210..>1273)
                     /note="from 2014 Arias et al. (Ganem lab) KSHV 2.0"
                     /label="PAN RNA - Ganem lab 2014"
     misc_RNA        complement(211..>1273)
                     /note="corresp. pos."
                     /label="PAN RNA - NC_009333 genome annotation"
     polyA_site      complement(469)
                     /note="corresp. pos."
                     /label="TTS K4.5(T6.1)-PAN - Zheng lab 2013"
     polyA_site      complement(821)
                     /note="corresp. pos."
                     /label="TTS K4.5(T6.1)-PAN-K7 - Zheng lab 2013"
     polyA_signal    complement(1263..>1273)
                     /note="corresp. pos."
                     /label="PAS K4.5-PAN-K7 - Gao lab 2014"
     Site            1268..1273
                     /site_type="mutagenized site"
                     /label="3' RE site"
ORIGIN
        1 GAATTCAATA ATTACAAGTA GGCATAACAC ATATACATTA AACCAAAGGT ATAAACCATC
       61 CTCATACCTC AAAAGAATAC CTAATAATTC CCAGTCGAAC TTCCTACTAC AATTACTGTA
      121 TTTCCCCGCA CCGAAGGTTT TTACCCACCG ATTGGACAGG TTTTATACCC TTGTGACCTC
      181 TATTTTCCCC GGTCGAACTC AGTCAAATCG TGACCCTGAC GGGTCAGTGG AACCGACGGC
      241 GAAGTGGATA CCTAAAACAC GAGCGACGAA CGGAAGAACG GCGAAGACCA AAAGTAACCA
      301 CGGCGGCTAA CACCCAACTA ACGCAGCGAA AACCGTTATA TGGGTAGGAC CGAAAGCCGA
      361 TCCAAAAGGC AGGATGAAAA GGGTGTAACC GGACTCTCGA CATCATGTTT TTTGTGGCGC
      421 GCCAGACCTC GAGAGGTATT CGGGCGTCTT GTTTTCGACG CTAAACGGGT TTTTGGAACG
      481 GTACCGTTGA TATGTCAGTG GGGAACGCCC AATAACGTAA CCTAAGTTAG AGGTCCGGTC
      541 AACATCGGGG GAAAATACTA TACGCTCCTA TGAATTGCAC AGACTTACAC CTTATATTAC
      601 ACTTTCCTTT CGTCGCGGGT GACCACATAG TCTTGTCACC ACGTGATGGA TAGACGAGTA
      661 AGCAACAAAG CCAAGACACA AACAGACTAA GAATCTATCA CAACTCCATT AAGATCTTTC
      721 GCCTAACTCA CATTTAGCCC GGTGAAACGG GATTTACACT GTTAGACCTA CACATAGAAT
      781 AACCACGCAA CACTTCGTAA AATTTTACGC AAAATCTAAC ATAGTCCGAT CACGACATTA
      841 CCACACAAAT AAAAAGGTCA CATTCGTTCA GCTAAACTTA CTGTATCCGC TGTTTCACTC
      901 CACCGTAAAC AGTCTTCAAA GTTTCAGCAC ATTCTTGTAA CCTGATTTCA CCACACGCCG
      961 TCGACCCTCG CGAGAAAGTT ACAATTACAA AATTACACAT ACAACACAAC CTTCAAGGTC
     1021 CGATTATAAA CTACAAAACG ATCCAACTGA TTGCTACAAA AGAACATCCA CTTTCGCAAC
     1081 ACATTGTTAC TATTGCCACA AAACCGACCC AAAAAGGAAC AAGCGTGGCC TGTGGAGGTC
     1141 ACTGGTCTGC CGTTCCAAAA ATAGGGTCAC ATATAACCTT TTTGTACAAT ATGAAAACTG
     1201 TTAAATTGCA CGGATCTCGA GTTTAATTTG ATTATGGTAT TGCATTACGT TGAATGTTGT
     1261 ATTTATTGGA TCC
//