LOCUS       pOME0302      1149 bp    DNA     linear   UNA 29-Jun-2016
DEFINITION  Zika NS1 with added start and stop codon TAGGED
FEATURES             Location/Qualifiers
     Site            1..6
                     /site_type="restriction site"
                     /label=KpnI
     Site            7..12
                     /site_type="mutagenized site"
                     /label=Kozak
     CDS             13..1146
                     /cds_type=ORF
                     /note="Length: 1128"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     CDS             17..229
                     /cds_type=ORF
                     /note="Length: 213"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     Site            1072..1137
                     /site_type="mutagenized site"
                     /label="3X FLAG"
     Site            1138..1143
                     /site_type="mutagenized site"
                     /label="Double stop"
     Site            1144..1149
                     /site_type="restriction site"
                     /label=XhoI
ORIGIN
        1 ggtaccGCCA CCATGGATGT GGGGTGCTCG GTGGACTTCT CAAAGAAGGA GACGAGATGC
       61 GGTACAGGGG TGTTCGTCTA TAACGACGTT GAAGCCTGGA GGGACAGGTA CAAGTACCAT
      121 CCTGACTCCC CCCGTAGATT GGCAGCAGCA GTCAAGCAAG CCTGGGAAGA TGGTATCTGT
      181 GGGATCTCCT CTGTTTCAAG AATGGAAAAC ATCATGTGGA GATCAGTAGA AGGGGAGCTC
      241 AACGCAATCC TGGAAGAGAA TGGAGTTCAA CTGACGGTCG TTGTGGGATC TGTAAAAAAC
      301 CCCATGTGGA GAGGTCCACA GAGATTGCCC GTGCCTGTGA ACGAGCTGCC CCACGGCTGG
      361 AAGGCTTGGG GGAAATCGTA CTTCGTCAGA GCAGCAAAGA CAAATAACAG CTTTGTCGTG
      421 GATGGTGACA CACTGAAGGA ATGCCCACTC AAACATAGAG CATGGAACAG CTTTCTTGTG
      481 GAGGATCATG GGTTCGGGGT ATTTCACACT AGTGTCTGGC TCAAGGTTAG AGAAGATTAT
      541 TCATTAGAGT GTGATCCAGC CGTTATTGGA ACAGCTGTTA AGGGAAAGGA GGCTGTACAC
      601 AGTGATCTAG GCTACTGGAT TGAGAGTGAG AAGAATGACA CATGGAGGCT GAAGAGGGCC
      661 CATCTGATCG AGATGAAAAC ATGTGAATGG CCAAAGTCCC ACACATTGTG GACAGATGGA
      721 ATAGAAGAGA GTGATCTGAT CATACCCAAG TCTTTAGCTG GGCCACTCAG CCATCACAAT
      781 ACCAGAGAGG GCTACAGGAC CCAAATGAAA GGGCCATGGC ACAGTGAAGA GCTTGAAATT
      841 CGGTTTGAGG AATGCCCAGG CACTAAGGTC CACGTGGAGG AAACATGTGG AACAAGAGGA
      901 CCATCTCTGA GATCAACCAC TGCAAGCGGA AGGGTGATCG AGGAATGGTG CTGCAGGGAG
      961 TGCACAATGC CCCCACTGTC GTTCCGGGCT AAAGATGGCT GTTGGTATGG AATGGAGATA
     1021 AGGCCCAGGA AAGAACCAGA AAGTAACTTA GTAAGGTCAA TGGTGACTGC Agactacaaa
     1081 gaccatgacg gtgattataa agatcatgat atcgattaca aggatgacga tgacaagtag
     1141 taactcgag
//