LOCUS       pOME0306       483 bp    DNA     linear   UNA 29-Jun-2016
DEFINITION  Zika NS2b with start/stop codons and kozak added TAGGED
FEATURES             Location/Qualifiers
     Site            1..6
                     /site_type="mutagenized site"
                     /label="5' RE site"
     Site            7..12
                     /site_type="mutagenized site"
                     /label=Kozak
     CDS             13..483
                     /cds_type=ORF
                     /note="Length: 396"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     CDS             13..405
                     /cds_type=ORF
                     /label=NS2B
     Site            406..471
                     /site_type="mutagenized site"
                     /label="3X FLAG"
     Site            472..477
                     /site_type="mutagenized site"
                     /label="Double stop"
     Site            478..483
                     /site_type="mutagenized site"
                     /label="3' RE site"
ORIGIN
        1 ggtaccgcca ccATGAGCTG GCCCCCTAGC GAAGTACTCA CAGCTGTTGG CCTGATATGC
       61 GCATTGGCTG GAGGGTTCGC CAAGGCAGAT ATAGAGATGG CTGGGCCCAT GGCCGCGGTC
      121 GGTCTGCTAA TTGTCAGTTA CGTGGTCTCA GGAAAGAGTG TGGACATGTA CATTGAAAGA
      181 GCAGGTGACA TCACATGGGA AAAAGATGCG GAAGTCACTG GAAACAGTCC CCGGCTCGAT
      241 GTGGCGCTAG ATGAGAGTGG TGATTTCTCC CTGGTGGAGG ATGACGGTCC CCCCATGAGA
      301 GAGATCATAC TCAAGGTGGT CCTGATGACC ATCTGTGGCA TGAACCCAAT AGCCATACCC
      361 TTTGCAGCTG GAGCGTGGTA CGTATACGTG AAGACTGGAA AAAGGgacta caaagaccat
      421 gacggtgatt ataaagatca tgatatcgat tacaaggatg acgatgacaa gtagtaactc
      481 gag
//