LOCUS pOME0306 483 bp DNA linear UNA 29-Jun-2016 DEFINITION Zika NS2b with start/stop codons and kozak added TAGGED FEATURES Location/Qualifiers Site 1..6 /site_type="mutagenized site" /label="5' RE site" Site 7..12 /site_type="mutagenized site" /label=Kozak CDS 13..483 /cds_type=ORF /note="Length: 396" /note="Found at strand: positive" /note="Start codon: ATG" CDS 13..405 /cds_type=ORF /label=NS2B Site 406..471 /site_type="mutagenized site" /label="3X FLAG" Site 472..477 /site_type="mutagenized site" /label="Double stop" Site 478..483 /site_type="mutagenized site" /label="3' RE site" ORIGIN 1 ggtaccgcca ccATGAGCTG GCCCCCTAGC GAAGTACTCA CAGCTGTTGG CCTGATATGC 61 GCATTGGCTG GAGGGTTCGC CAAGGCAGAT ATAGAGATGG CTGGGCCCAT GGCCGCGGTC 121 GGTCTGCTAA TTGTCAGTTA CGTGGTCTCA GGAAAGAGTG TGGACATGTA CATTGAAAGA 181 GCAGGTGACA TCACATGGGA AAAAGATGCG GAAGTCACTG GAAACAGTCC CCGGCTCGAT 241 GTGGCGCTAG ATGAGAGTGG TGATTTCTCC CTGGTGGAGG ATGACGGTCC CCCCATGAGA 301 GAGATCATAC TCAAGGTGGT CCTGATGACC ATCTGTGGCA TGAACCCAAT AGCCATACCC 361 TTTGCAGCTG GAGCGTGGTA CGTATACGTG AAGACTGGAA AAAGGgacta caaagaccat 421 gacggtgatt ataaagatca tgatatcgat tacaaggatg acgatgacaa gtagtaactc 481 gag //